Abstract
Development of cotyledonary-stage nucellar embryos of mango was arrestedin vitro by exposure to 750–1750 μM ABA. The enlargement and germination of nucellar embryos was inhibited for as long as 4 weeks after subculture from ABA-containing medium. Mannitol at concentrations between 7.5 and 12.5% inhibited nucellar embryo development, presumably due to osmotic effects; however, there was no residual effect after subculture of somatic embryos onto medium without mannitol. Temperatures between 22.5 and 37.5°C stimulated embryo development, whereas lower temperatures (7.5 and 15°C) delayed germination. There was no germination 1 month after somatic embryos, pulsed for 8 weeks at 7.5°C, were transferred to 22.5°C; however, after 2 months, 86% of these somatic embryos germinated. These results indicate that it is possible to induce developmental arrest in recalcitrant mango embryos with high concentrations of ABA, mannitol or low temperature (7.5°C).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- ABA:
-
Abscisic acid
- MM1:
-
Mango maturation medium
References
Attree SM & Fowke LC (1993) Embryogeny of gymnosperms: advances in synthetic seed technology of conifers. Plant Cell Tiss. Org. Cult. 35: 1–35
Carman JG (1988) Improved somatic embryogenesis in wheat by partial simulation of thein ovulo oxygen, growth regulators and desiccation environments. Planta 175: 417–424
Chandler PM, Walker-Simmons M, King RW, Crouch M & Close TJ (1988) Expression of ABA-inducible genes in water-stressed cereal seedlings. J. Cell. Biochem. (Suppl. 12C): 143
Chin HF (1988) Recalcitrant Seeds A Status Report, International Board for Plant Genetic Resources, Rome
Chin HF & Roberts EH (1980) Recalcitrant Crop Seeds, Tropical Press, Kuala Lumpur
DeWald SG, Litz RE & Moore GA (1989) Maturation and germination of mango somatic embryos. J. Amer. Soc. Hort. Sci. 114: 837–841
Etienne H, Montoro P, Michaux-Ferriere N & Carron MP (1993) Effects of desiccation, medium osmolarity and abscisic acid on the maturation ofHevea brasiliensis somatic embryos. J. Exp. Bot. 44: 1613–1619
Fujii JAA, Slade D & Redenbaugh K (1989) Maturation and greenhouse planting of alfalfa artificial seeds. In Vitro Cell. Dev. Biol. 25: 1179–1182
Gamborg OL, Miller RA & Ojima K (1968) Plant cell cultures. I. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 150–158
Gray DJ & Purohoit A (1991) Somatic embryogenesis and development of synthetic seed technology. Crit. Rev. Plant Sci. 10: 33–61
Hetherington AM & Quatrano RS (1991) Mechanisms of action of abscisic acid at the cellular level. New Phytol. 119: 9–32
Karssen CM, Brinkhorst van der Swan DLC, Breekland AE & Koornheef M (1983) Induction of dormancy during seed development by endogenous abscisic acid: studies on abscisic acid deficient genotypes ofArabidopsis thaliana (L.) Heynh. Planta 157: 158–165
Kermode AR (1990) Regulatory mechanisms involved in the transition from seed development to germination. CRC Crit. Rev. Plant Sci. 9: 155–195
King MW & Roberts EH (1979) The Storage of Recalcitrant Seeds. Achievements and Possible Approaches, International Board for Plant Genetic Resources, Rome
Koornheef M, Hanhart CJ, Hilhorst HWM & Karssen CM (1989)in vivo inhibition of seed development and reserve protein accumulation in recombinants of abscisic acid biosynthesis and responsiveness mutants inArabidopsis thaliana. Plant Physiol. 90: 463–469
Kriz R, Wallace MS & Paiva R (1990) Globulin gene expression in embryos of maizeviviparous mutants. Evidence for regulation of the Glb 1 gene by ABA. Plant Physiol. 92: 538–542
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Parisot E (1988) Etude de la croissance rhythmique chez de jeunes manguiers (Mangifera indica L.). Description, germination et conservation de graines polyembryonnees de manguier. Fruits 43: 97–105
Pence V C (1991) Abscisic acid in developing zygotic embryos ofTheobroma cacao. Plant Physiol. 95: 1291–1293
Pence V C (1992) Abscisic acid and the maturation of cacao embryosin vitro. Plant Physiol. 98: 1391–1395
Pia M, Goday A, Vilardell J, Gomez J & Pages M (1989) Differential regulation of the ABA-induced 23–25 kD proteins in embryos and vegetative tissues of the viviparous mutants of maize. Plant Mol. Biol. 13: 385–394
Quatrano RS (1987) The role of hormones during seed development. In: Davis PJ (ed) Plant Hormones and Their Role in Plant Growth and Development (pp 494–514). Martinus Nijhoff, Dordrecht
Roberts E H (1973) Predicting the storage life of seeds. Seed Sci. Technol. 1: 499–514
Sussex I (1975) Growth and metabolism of the embryo and attached seedling of the viviparous mangrove,Rhizophora mangle. Amer. J. Bot. 62: 948–953
Welbaum G E, Tissaoui T & Bradford K J (1990) Water relations of seed development and germination in muskmelon (Cucumis melo L.). III. Sensitivity of germination to water potential and abscisic acid during development. Plant Physiol. 92: 1029–1037
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pliego-Alfaro, F., Litz, R.E., Moon, P.A. et al. Effect of abscisic acid, osmolarity and temperature onin vitro development of recalcitrant mango nucellar embryos. Plant Cell Tiss Organ Cult 44, 53–61 (1996). https://doi.org/10.1007/BF00045913
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00045913