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Isolation of a cytochrome P450 homologue preferentially expressed in developing inflorescences ofZea mays

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Abstract

Four cDNA clones exhibiting preferential hybridization to transcripts present in developing maize tassels were isolated by differential screening. One of these cDNA clones hybridizes to transcripts detectable only in the shoot apex. The abundance of this transcript is significantly higher in developing inflorescence apices than in vegetative apices. DNA sequence analysis of a 2107 nucleotide cDNA clone corresponding to this transcript revealed that the transcript encodes a polypeptide of 547 amino acids, with a molecular mass of 58.4 kDa. This polypeptide shares significant sequence similarity with members of the cytochrome P450 monooxygenase gene superfamily, including the conserved C-terminal domains typical of the cytochrome P450 monooxygenases.

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  1. John C. Larkin

    Present address: Dept. of Genetics and Cell Biology, University of Minnesota, 1445 Gortner Ave., 55108, St. Paul, MN, USA

Authors and Affiliations

  1. Department of Biochemistry, University of Minnesota, 35108, St. Paul, MN, USA

    John C. Larkin

  2. Plant Molecular Genetics Institute, University of Minnesota, 35108, St. Paul, MN, USA

    John C. Larkin

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Larkin, J.C. Isolation of a cytochrome P450 homologue preferentially expressed in developing inflorescences ofZea mays . Plant Mol Biol 25, 343–353 (1994). https://doi.org/10.1007/BF00043864

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  • DOI: https://doi.org/10.1007/BF00043864

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