Abstract
Node cultures ofGypsophila paniculata showed vitrified growth when cultured under standard conditions, resulting in poor survival of tissue culture derived plants. The deviant growth pattern appeared to be determined in the lag phase of the cultures. A limited evaporation period in this stage induced normal growth. Re-establishment of high relative humidity and poorer gas exchange thereafter did not lead to vitrified growth. Vitrification was also depressed when evaporation was continued during the whole culture period, but shorter plantlets resulted. A simple adaptation of the plastic lid that is commonly used in micropropagation, allowed controlled and contamination-free evaporation.
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Dillen, W., Buysens, S. A simple technique to overcome vitrification inGypsophila paniculata L.. Plant Cell Tiss Organ Cult 19, 181–188 (1989). https://doi.org/10.1007/BF00043345
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DOI: https://doi.org/10.1007/BF00043345