Abstract
A system of in vitro clonal propagation has been developed in Pisum sativum L. (cv. Bohatýr). A modified MS-medium supplemented with 20 μM 6-benzylaminopurine (BAP) and 0.1 μM α-naphthaleneacetic acid (NAA) was used to induce multiple shoot formation from shoot apices, axillary buds of the first normal leaf, axillary buds of the first and second primary scales and axillary buds of cotyledons of 4 to 6 day old pea seedlings. Meristem explants maintained a high proliferation ability in each subculture in the course of 20 months of the culture. Regenerated shoots were rooted in the same basal medium containing 5 μM NAA. Rooted plants were cultured in hydroponic pots filled with half-strength MS-medium to attain anthesis and seed maturity. The phenotypic uniformity of the regenerants was evaluated. Cytological investigation confirmed the diploid stage (2n=14) of regenerants and their progeny. Histological studies revealed that proliferating shoots originated from axillary and adventitious buds. In vitro propagation is discussed as related to pea breeding.
Similar content being viewed by others
References
Conger AD, Fairchild LM (1953) A quick freeze method for making smear glides permanent. Stain Technol. 28: 281–283
Davies DR (1983) Chimerism in irradiated dicotyledonous plants. In: Chimerism in irradiated dicotyledonous plants. Report of a consultants meeting of Joint FAO/IAEA Div. 1981. Vienna: IAEA, p 13–17
Gamborg OL, Constabel F, Shyluk JP (1974) Organogenesis in callus from shoot apices of Pisum sativum. Physiol. Plant. 30: 125–128
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements for suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158
Griga M, Tejklová E, Novák FJ (1984) Hormonal regulation of growth of pea (Pisum sativum L.) shoot apices in in vitro culture. Rostl. Výr. 30: 523–530 (in Czech)
Kartha KK (1981) Meristem culture and cryopreservation. Methods and applications. In: Thorpe TA, ed, Plant tissue culture methods and applications in agriculture. New York: Academic Press, p 181–211
Kartha KK, Gamborg OL (1978) Meristem culture techniques in the production of disease-free plants and freeze-preservation of germplasm of tropical tuber crops and grain legumes. In: Maraite H, Meyer JA eds, Disease of tropical food crops. Université Catholique de Louvain, Belgium, p 267–283
Kartha KK, Gamborg OL, Constabel F (1974) Regeneration of pea (Pisum sativum L.) plants from shoot apical meristems. Z. Pflanzenphysiol. 72: 172–176
Kartha KK, Leung NL, Gamborg OL (1979) Freeze preservation of pea meristems in liquid nitrogen and subsequent plant regeneration. Plant Sci. Lett. 15: 7–15
Mroginski LA, Kartha KK (1981) Regeneration of pea (Pisum sativum L. cv. Century) plants by in vitro culture of immature leaflets. Plant Cell Reports 1: 64–66
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Němec B (1962) Botanická mikrotechnika. Praha: Nakl. ČSAV
Rubluo A, Kartha KK, Mroginski LA, Dyck J (1984) Plant regeneration from pea leaflets cultured in vitro and genetic stability of regenerants. J. Plant Physiol. 117: 119–130
Rubluo A, Mroginski L, Kartha KK (1982) Morphogenetic responses of pea leaflets cultured in vitro. In: Fujiwara A, ed, Plant Tissue Culture 1982. Tokyo: The Japanese Assoc. Plant Tissue Culture, p 151–152
Saccardo F (1983) Chimera formation in M1 pea plants raised from mutagentreated seeds. In: Chimerism in irradiated dicotyledonous plants. Report of a consultants' meeting of Joint FAO/IAEA Div. 1981. Vienna: IAEA, p 23–24
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Griga, M., Tejklová, E., Novák, F.J. et al. In vitro clonal propagation of Pisum sativum L.. Plant Cell Tiss Organ Cult 6, 95–104 (1986). https://doi.org/10.1007/BF00037762
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00037762