Summary
Roots, leaf petioles, shoot tips and immature fruits of female date palm formed the source material for in vitro cultures. Isolated embryos excised from mature seeds were also cultured. Of all the tissues, some of the leaf petioles and fruit mesocarp gave rise to calluses in the media of Staritsky (1970) and Eeuwens (1976). Isolated embryos germinated to produce seedlings in MS (Murashige & Skoog, 1962) medium. Calluses were established from seedling sections but the growth conditions propitious for explants of seedlings were not suitable for female date palm tissues. The main problem in establishing long term tissue cultures has been the browning of the tissues and media. This problem was, at least partially, overcome by adding activated charcoal to the liquid media and cysteine to the solid madia. So far, shoot regeneration in the calluses could not be induced.
This is a preview of subscription content, log in via an institution to check access.
References
Ammar S. & A. Benbadis, 1977. Multiplication vegetative du palmier-dattier (Phoenix dactylifera L.) par la culture de tissues de jeunes issues de semis. C. R. Acad. Sci. Paris 284: 1789–1792.
Anonymous, 1976. Report of the Date Palm Study Team to Middle East Countries and United States of America, 1976. Indian Council of Agric. Res., New Delhi, India.
Bhojwani S. S., P. K. Evans & E. C. Cocking, 1977. Protoplast technology in relation to crop plants: Progress and problems. Euphytica 26: 343–360.
Eeuwens C. J., 1976. Mineral requirements for growth and callus initiation of tissue explants excised from mature coconut palms (Cocos nucifera) and cultured in vitro. Physiol. Plantarum 36: 23–28.
Eeuwens C. J., 1978. Effects of organic nutrients and hormones on growth and development of tissue explants from coconut (Cocos nucifera) and date (Phoenix dactylifera) palms cultured in vitro. Physiol. Plantarum 42: 173–178.
Hussey G., 1978. The application of tissue culture to the vegetative propagation of plants. Science Progress 65: 185–208.
King P. J., 1974. Economic developments from plant tissue culture. Biologist 21: 87–93.
Murashige T., 1974. Plant propagation through tissue cultures. Ann. Rev. Plant Physiol. 25: 135–165.
Murashige T. & F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum 15: 473–497.
Nitsch J. P. & C. Nitsch, 1969. Haploid plants from pollen grains. Science 163: 85–87.
Reuveni O., Y. Adato & H. Lilien-Kipnis, 1972. A study of new and rapid methods for vegetative propagation of date palms. Date Grogers Inst. Report. 49: 17–24.
Reuveni O. & H. Lilien-Kipnis, 1974. Studies on the invitro culture of date palm (Phoenix dactylifera L.) tissues and organs. Pamphlet No. 145. Volcani Inst. Agric. Res., Israel.
Schenk R. U. & A. C. Hildebrandt, 1972. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Botany, 50: 199–204.
Schroeder, C. A., 1972. Tissue culture of date palm shoots and seedlings. Date Growers' Inst. Report No. 49: 25–27.
Staritsky G., 1970. Tissue culture of the oil palm (Elaeis guineensis Jacq.) as a tool for its vegetative propagation. Euphytica 19: 288–292.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sharma, D.R., Kumari, R. & Chowdhury, J.B. In vitro culture of female date palm (Phoenix dactylifera L.) tissues. Euphytica 29, 169–174 (1980). https://doi.org/10.1007/BF00037263
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00037263