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Characterization of photosystem II in stroma thylakoid membranes

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Abstract

The functional state of the PS II population localized in the stroma exposed non-appressed thylakoid region was investigated by direct analysis of the PS II content of isolated stroma thylakoid vesicles. This PS II population, possessing an antenna size typical for PS IIβ, was found to have a fully functional oxygen evolving capacity in the presence of an added quinone electron acceptor such as phenyl-p-benzoquinone. The sensitivity to DCMU for this PS II population was the same as for PS II in control thylakoids. However, under more physiological conditions, in the absence of an added quinone acceptor, no oxygen was evolved from stroma thylakoid vesicles and their PS II centers were found to be incapable to pass electrons to PS I and to yield NADPH. By comparison of the effect of a variety of added quinone acceptors with different midpoint potentials, it is concluded that the inability of PS II in the stroma thylakoid membranes to contribute to NADPH formation probably is due to that QA of this population is not able to reduce PQ, although it can reduce some artificial acceptors like phenyl-p-benzoquinone. These data give further support to the notion of a discrete PS II population in the non-appressed stroma thylakoid region, PS IIβ, having a higher midpoint potential of QA than the PS II population in the appressed thylakoid region, PS IIα. The physiological significance of a PS II population that does not produce any NADPH is discussed.

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Abbreviations

pBQ:

p-benzoquinone

Chl:

chlorophyll

DCBQ:

2,6-dichloro-p-benzoquinone

DCIP:

2,6-dichloroindophenol

DCMU:

3-(3′,4′-dichlorophenyl)-1,1-dimethylurea

DMBQ:

2,5-dimethyl-p-benzoquinone

DQ:

duroquinone(tetramethyl-p-benzoquinone)

FeCN:

ferricyanide (potassium hexacyanoferrat)

MV:

methylviologen

NADPH,NADP+ :

reduced or oxidized form of nicotinamide adenine dinucleotide phosphate respectively

PpBQ:

phenyl-p-benzoquinone

PQ:

plastoquinone

PS II:

photosystem II

PS I:

photosystem I

QA :

primary quinone acceptor of PS II

QB :

secondary quinone acceptor of PS II

μE:

microEinstein

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Authors and Affiliations

  1. Department of Plant Biochemistry, University of Lund, P.O. Box 124, S-221 00, LUND, Sweden

    Tomas Henrysson & Cecilia Sundby

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  1. Tomas Henrysson
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  2. Cecilia Sundby
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Henrysson, T., Sundby, C. Characterization of photosystem II in stroma thylakoid membranes. Photosynth Res 25, 107–117 (1990). https://doi.org/10.1007/BF00035459

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  • DOI: https://doi.org/10.1007/BF00035459

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