Abstract
Vitrification of internodes of carnation was brought about by culturing in liquid medium. Cell wall extensibility of these internodes was kinetically followed in comparison to that of normal plants using the constant stress method. Liquid culture induced increased immediate and total deformation capacities of the walls from the second day. Measurements indicated that these deformation capacities involved plastic properties rather than elastic ones. These changes were paralleled by decreased relative levels of cellulose and lignin.
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Alibert G and Boudet A (1979) La lignification chez le peuplier.I. Mise au point d'une méthose de dosage et d'analyse monomérique des lignines. Physiol Vég 17: 67–74
Anonymous (1985) Regeneration and vitrification: Role of ethylene. Agricell Report, 14–15 August
Boyer N, Gaspar Th and Lamond M (1979) Modifications des isoperoxydases et de I'allongement des entre-noeuds de Bryone à la suite d'irritations mécaniques. Z Pflanzenphysiol 93: 459–470
Cleland RE (1967) Extensibility of isolated cell walls: Measurements and changes during cell elongation. Planta 74: 197–209
Cleland RE (1981) Wall extensibility: Hormones and wall extension In: W Tanner and F Loewus, eds Encyclopedia of Plant Physiology, new series, Vol 13 B Plant carbohydrates II pp 255–269. Berlin, Heidelberg, New York: Springer Verlag
Cleland RE (1983) The capacity for acid-induced wall loosening as a factor in the control of Avena coleoptile cell elongation. J Exp Bot 34: 676–680
Coartney JS and Morré DJ (1980) Studies on the role of wall extensibility in the control of cell expansion. Bot Gaz 141: 56–62
De Proft MP, Van den Broek G and De Greef JA (1986) Involvement of ethylene on senescence and vitrification of in vitro cultured miniroses. Acta Hort: In press
Earle E and Langhans R (1975) Carnation propagation from shoot tips cultured in liquid medium. Hort Sci 10: 608–610
Gaspar Th, Kevers C, Debergh P, Maene L, Paques M and Boxus P (1986) Vitrification: Morphological, physiological and ecological aspects. In JMBonga and DJDurzan, eds. Tissue Culture in Forestry, in press, The Hague, Boston, London: Martinus Nijhoff/Dr W Junk
Goldberg R, Catesson AM and Czaninski Y (1983) Some properties of syringaldazine oxidase: a peroxidase specifically involved in the lignification processes. Z Pflanzenphysiol 110: 267–279
Hedegus P and Phan CT (1983) Actions de phénols sur les malformations observées chez les porte-greffes de pommiers M-26 et 0–3 cultivés in vitro. Rev Can Biol Exptl 42: 33–38
Johnson DB, Moore WE and Zank LC (1961) The spectrophotometric determination of lignins in small wood samples. TAPPI (Technical Association of the Pulp and Pepper Industry) 44: 793–798
Kevers C, Coumans M, Coumans-Gillès MF, Gaspar Th (1984) Physiological and biochemical events leading to vitrification of plants cultured in vitro. Physiol Plant 61: 69–74
Kevers C and Gaspar Th (1985a) Soluble, membrane and wall peroxidases, phenylalanine ammonia-lyase, and lignin changes in relation to vitrification of carnation tissues cultured in vitro. J Plant Physiol 118: 41–48
Kevers C and Gaspar Th (1985b) Vitrification of carnation in vitro: Changes in ethylene production, ACC level and capacity to convert ACC to ethylene. Plant Cell Tissue Organ Culture 4: 215–223
Letouze R and Daguin F (1983) Manifestation spontanée et aléatoire d'une croissance anormale en culture in vitro. Recherche de marqueurs métaboliques. Rev Can Biol Exptl 42: 23–28
Marigo M and Boudet AM (1980) Relation polyphénols-croissance, lignification et limitation de croissance chez Lycopersicum esculentum. Physiol Plant 49: 425–430
Paques M and Boxus Ph (1984) Comparative study of vitreous and non vitreous plantlets of apple rootstock M-26 cultivated in vitro. Abstr Book, 4 th Congr Fed Eur Soc Plant Physiol, Strasbourg pp 282–283
Phan CT and Letouze R (1983) A comparative study of chlorophyll, phenolic and protein contents, and of hydroxycinnamate: CoA ligase activity of normal and vitreous plants (Prunus avium L.) obtained in vitro. Plant Sci Lett 31: 323–327
Prat R and Goldberg R (1984) Relationships between auxin- and acid-induced growth in Vigna radiata hypocotyl segments. Physiol Plant 61: 51–57
Roberts LW and Miller AR (1983) Is ethylene involved in xylem differentiation? Vistas Plant Sci 6: 1–24
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Kevers, C., Prat, R. & Gaspar, T. Vitrification of carnation in vitro: Changes in cell wall mechanical properties, cellulose and lignin content. Plant Growth Regul 5, 59–66 (1987). https://doi.org/10.1007/BF00035020
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DOI: https://doi.org/10.1007/BF00035020