Abstract
Changes in insoluble or cell wall invertase and soluble invertase activity were examined during callus induction from tobacco pith-phloem explants and during callus proliferation on sucrose, glucose and fructose as carbon sources, or on transfer from culture on the hexoses to sucrose. In all cases there was a growth independent transitory increase in cell wall invertase early in culture. The magnitude of the increase was greatest in the presence of sucrose. Cell wall invertase was found to possess catalytic activity in situ, whether or not the tissue was grown on sucrose. It is hypothesized that the transitory increase in cell wall invertase plays a role in sucrose hydrolysis during wound respiration, which takes place early in culture.
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References
Biondi S, Thorpe TA (1982) Growth regulator effects, metabolite changes, and respiration during shoot initiation in cultured cotyledon explants of Pinus radiata. Bot Gaz 143: 20–25
Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254
Copping LG, Street HE (1972) Properties of the invertases of cultured sycamore cells and changes in their activity during culture growth. Physiol Plant 26: 346–354
Fowler MW (1978) Regulation of carbohydrate metabolism in cell suspension cultures. In: Thorpe TA (ed). Frontiers of plant tissue culture. Calgary: IAPTC, pp 443–452
Hisajima S (1975) The conversion of external sugars to internal sucrose in morning-glory callus. (in Japanese) Radioisotopes 24: 403–409
Komamine A, Shimizu T, Ashihara H, Shimokoriyama M (1972) The mechanism of changes in respiratory activity during callus formation in carrot slices cultured in vitro. Plant Cell Physiol 13: 821–829
Maretzki A, Thom M, Nickell LG (1974) Utilization and metabolism of carbohydrates in cell and tissue culture. In: Street HE (ed) Tissue culture and plant science, New York: Academic Press, pp 329–361
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473–497
Parr DR, Edelman J, Hawker JS (1976) Growth and sucrose metabolism of carrot callus strains with normal and low acid invertase activity. Physiol Plant 37: 223–228
Ross MK, Thorpe TA (1973) Physiological gradients and shoot initiation in tobacco callus. Plant Cell Physiol 14: 473–480
Straus J (1962) Invertase in cell walls of plant tissue cultures. Plant Physiol 37: 342–348
Thorpe TA (1982) Carbohydrate utilization and metabolism. In: Bonga JM, Durzan DJ (eds) Tissue culture in forestry. The Hague: Martinus Nijhoff/Dr. W. Junk, pp. 325–368
Thorpe TA, Meier DD (1972) Starch metabolism, respiration, and shoot formation in tobacco callus cultures. Physiol Plant 27: 365–369
Thorpe TA, Meier DD (1973) Sucrose metabolism during tobacco callus growth. Phytochem 12: 493–497
Ueda Y, Ishiyama H, Fukui M, Nishi A (1974) Invertase in cultured Daucus carota cells. Phytochem 13: 383–387
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Obata-Sasamoto, H., Thorpe, T.A. Cell wall invertase activity in cultured tobacco tissues. Plant Cell Tiss Organ Cult 2, 3–9 (1982). https://doi.org/10.1007/BF00033546
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DOI: https://doi.org/10.1007/BF00033546