Abstract
A non-detergent photosystem II preparation, named BS, has been characterized by countercurrent distribution, light saturation curves, absorption spectra and fluorescence at room and at low temperature (−196°C). The BS fraction is prepared by a sonication-phase partitioning procedure (Svensson P and Albertsson P-Å, Photosynth Res 20: 249–259, 1989) which removes the stroma lamellae and the margins from the grana and leaves the appressed partition region intact in the form of vesicles. These are closed structures of inside-out conformation. They have a chlorophyll a/b ratio of 1.8–2.0, have a high oxygen evolving capacity (295 μmol O2 per mg chl h), are depleted in P700 and enriched in the cytochrome b/f complex. They have about 2 Photosystem II reaction centers per 1 cytochrome b/f complex.
The plastoquinone pool available for PS II in the BS vesicles is 6–7 quinones per reaction center, about the same as for the whole thylakoid. It is concluded, therefore, that the plastoquinone of the stroma lamellae is not available to the PS II in the grana and that plastoquinone does not act as a long range electron transport shuttler between the grana and stroma lamellae.
Compared with Photosystem II particles prepared by detergent (Triton X-100) treatment, the BS vesicles retain more cytochrome b/f complex and are more homogenous in their surface properties, as revealed by countercurrent distribution, and they have a more efficient energy transfer from the antenna pigments to the reaction center.
Similar content being viewed by others
Abbreviations
- DCMU:
-
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- Fv :
-
variable fluorescence
- LHC:
-
light-harvesting complex
- PpBQ:
-
phenyl-p-benzoquinone
- PQ:
-
plastoquinone pool
- P700:
-
reaction center of PS I
- PS I, PS II:
-
Photosystem I, II
- QA :
-
first bound plastoquinone accepter
- RC:
-
reaction centre
References
Åkerlund H-E (1984) An apparatus for counter-current distribution in a centrifugal acceleration field. J Biochem Biophys Methods 9: 133–141
Albertsson P-Å (1985) Subfractionation of inside-out thylakoid vesicles and the localization of cytochrome f. Physiol Veg 23: 731–739
Albertsson P-Å (1986) Partition of Cell Particles and Macromolecules, 3rd ed. Wiley, New York
Albertsson P-Å (1988) Analysis of the domain structure of membranes by fragmentation and separation in aqueous polymer two-phase systems. Quart Rev Biophysics 21: 61–98
Albertsson P-Å and Yu SG (1988) Heterogeneity among Photosystem IIα. Isolation of thylakoid membrane vesicles with different functional antennae size of Photosystem IIα. Biochim Biophys Acta 936: 215–221
Albertsson P-Å, Yu SG and Larsson UK (1990a) Heterogeneity in Photosystem IIα. Evidence from fluorescence and gel electrophoresis experiments. Biochim Biophys Acta 1016: 137–140
Albertsson P-Å, Andreasson E and Svensson P (1990b) The domain organization of the plant thylakoid membrane. FEBS Lett 273: 36–40
Albertsson P-Å, Andreasson E, Persson A and Svensson P (1990c) Organization of the thylakoid membrane with respect to the four photosystems, PS Iα, PS Iβ, PS IIα and PS IIβ. In: Baltscheffsky M(ed) Current Research in Photosynthesis, Vol II, pp 923–925. Kluwer Academic Publishers, Dordrecht
Albertsson P-Å, Andreasson E, Svensson P and Yu SG (1991) Localization of cytochrome f in the thylakoid membrane: Evidence for multiple domains. Biochim Biophys Acta 1098: 90–94
Andersson B and Åkerlund HE (1978) Inside-out membrane vesicles isolated from spinach thylakoids. Biochim Biophys Acta 503: 462–472
Anderson JM and Melis A (1983) Localization of different photosystems in separate regions of chloroplast membranes. Proc Natl Acad Sci USA 80: 745–749
Andreasson E, Svensson P, Weibull C and Albertsson P-Å (1988) Separation and characterization of stroma and grana membranes-evidence for heterogeneity in antenna size of both Photosystem I and Photosystem II. Biochim Biophys Acta 936: 339–350
Berthold DA, Babcock GT and Yocum CF (1981) A highly resolved, oxygen-evolving Photosystem II preparation from spinach thylakoid membranes. FEBS Lett 134: 231–234
Björn GS, Braune W and Björn LO (1983) Photochromic pigments in akinetes and pigment characteristics of akinetes in comparison with vegetative cell of Anabaena variabilis. Physiol Plant 59: 493–500
Dekker JP, Bowlby NR and Yocum CF (1989) Chlorophyll and cytochrome b-559 content of the photochemical reaction center of Photosystem II. FEBS Lett 254: 150–154
Ghanotakis DF, dePaula JC, Demetriou DM, Bowlby NR, Petersen J, Babcock GT and Yocum CF (1989) Isolation and characterization of the 47 kDa protein and the D1–D2-cytochrome b-559 complex. Biochim Biophys Acta 974: 44–53
Hill R and Bendall F (1960) Function of the two cytochrome components in chloroplasts: A working hypothesis. Nature 186: 136
Hsu B-D and Lee J-Y (1991) Characterization of the Photosystem II centers inactive in plastoquinone reduction by fluorescence induction. Photosynth Res 27: 143–150
Joliot P and Joliot A (1992) Electron transfer between Photosystem II and the cytochrome b/f complex: Mechanistic and structural implications. Biochim Biophys Acta 1102: 53–61
Joliot P, Lavergne J and Beal D (1992) Plastoquinone compartmentation in chloroplasts. I. Evidence for domains with different rates of photo-reduction. Biochim Biophys Acta 1101: 1–12
Juhler RK, Andreasson E, Yu SG and Albertsson P-Å (1993) Composition of photosynthetic pigments in thylakoid membrane fractions from spinach. Photosynth Res 35: 171–178
Larsson UK, Ögren E, Öquist G and Andersson B (1986) Electron transport and fluorescence studies on the functional interaction between phospho-LHC II and Photosystem I in isolated stroma lamellae vesicles. Photobiochem Photobiophys 13: 29–39
Lavergne J and Leci E (1993) Properties of inactive Photosystem II centers. Photosynth Res 35: 323–343
Melis A (1985) Functional properties of Photosystem II in spinach chloroplasts. Biochim Biophys Acta 808: 334–342
Melis A and Homan PH (1975) Kinetic analysis of the fluorescence induction in 3-(3,4-dichlorophenyl)-1, 1 dimethylurea poisoned chloroplasts. Photochem Photobiol 23: 431–437
Melis A and Homan PH (1975) A selective effect of Mg2+ on the photochemistry at one type of reaction center in Photosystem II of chloroplasts. Arch Biochem Biophys 190: 523–536
Newell WR, vanAmerongen H, Barber J and vanGrondelle R (1991) Spectroscopic characteristation of the reaction centre of Photosystem II using polarised light: Evidence for β-carotene excitons in PS II reaction centres. Biochim Biophys Acta 1057: 232–238
Pålsson LO, Gilbro T, Svensson P and Albertsson P-Å (1990) Interaction between LHC II antenna and PS 2 core in thylakoid vesicles. In: Baltscheffsky M (ed) Current Research in Photosynthesis, Vol II, pp 301–304. Kluwer Academic Publishers, Dordrecht
Pijgersberg CP, Amesz J, Thielen APGM and Swager JA (1979) Fluorescence emission spectra of chloroplasts and subchloroplast preparations at low temperature. Biochim Biophys Acta 545: 473–482
Svensson P and Albertsson P-Å (1989) Preparation of highly enriched Photosystem II membrane vesicles by a nondetergent method. Photosynth Res 20: 249–259
Svensson P, Yu SG, Larsson UK, Andreasson E and Albertsson P-Å (1990) in: Baltscheffsky M (ed) Current Research in Photosynthesis, Vol II, pp 839–842. Kluwer Academic Publishers, Dordrecht
Tang D, Jankowiak R, Seibert M and Small GJ (1991) Effects of detergent on the excited state structure and relaxation dynamics of the Photosystem II reaction center: A high resolution hole burning study. Photosynth Res 27: 19–29
VanDorssen RJ, Plijter JJ, Dekker JP, Oudenden A, Amesz J and vanGorkom HJ (1987) Spectroscopic properties of chloroplast grana membrancs and of the core of Photosystem II. Biochim Biophys Acta 890: 134–143
Yu SG, Andreasson E and Albertsson P-Å (1990) Photosynthetic characterization of thylakoid membrane preparations isolated from spinach grown under different light conditions. In: Baltscheffsky M (ed) Current Research in Photosynthesis, Vol II, 8, pp 843–846. Kluwer Academic Publishers, Dordrecht
Yu SG, Stefansson H and Albertsson P-Å (1992) Localization of 64 kDa LHC II-kinase in the thylakoid membrane from spinach. In: Murata N (ed) Research in Photosynthesis, Vol I, pp 2.283–286. Kluwer Academic Publishers, Dordrecht
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yu, SG., Björn, G. & Albertsson, PÅ. Characterization of a non-detergent PS II-cytochrome b/f preparation (BS). Photosynth Res 37, 227–236 (1993). https://doi.org/10.1007/BF00032826
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00032826