Skip to main content
SpringerLink
Log in

The 28 kDa apoprotein of CP 26 in PS II binds copper

  • Regular Paper
  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

Photosystem II (PS II) particles isolated from spinach in the presence of 10 μM CuSO4 contained 1.2 copper/300 Chl that was resistant to EDTA. When CuSO4 was not added during the isolation, PS II particles contained variable amounts of copper resistant to EDTA (0.1–1.1 copper/300 Chl). No correlation was found between copper content and oxygen evolving capacity of the PS II particles. To identify the copper binding protein, we developed a fractionation procedure which included solubilisation of PS II particles followed by precipitation with polyethylene glycol. A 22-fold purification of copper with respect to protein was achieved for a 28 kDa protein. Partial amino acid sequence of a 13 kDa fragment, obtained after V8 (endo Glu-C) protease treatment, showed identity with CP 26 over a 14 amino acid stretch. EPR measurements on the purified protein suggest oxygen and/or nitrogen as ligands for copper but tend to exclude sulfur. We conclude that the 28 kDa apoprotein of CP 26 from spinach binds one copper per molecule of CP 26. A possible function for this copper protein in the xanthophyll cycle is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CP 26 and CP 29:

chlorophyll a/b protein complex 26 and 29

LHC II:

light-harvesting chlorophyll a/b protein complex of Photosystem II

SB14:

sulfobetaine 14

References

  • Aebersold R (1989) Internal amino acid sequence analysis of proteins after ‘in situ’ protease digestion on nitrocellulose. In: Matsudaira PT (ed) A Practical Guide to Protein and Peptide Purification for Microsequencing, pp 71–88. Academic Press, San Diego

    Google Scholar 

  • Åkerlund H-E and Jansson C (1981) Localization of a 34 000 and a 23 000 Mr polypeptide to the lumenal side of the thylakoid membrane FEBS Lett 124: 229–232

    Article  Google Scholar 

  • Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24: 1–15

    Google Scholar 

  • Barón M, Lachica M, Chueca A and Sandmann G (1990) The role of copper in the structural organization of Photosystem II in chloroplast membranes. In: Baltscheffsky M (ed) Current Research in Photosynthesis, Vol I, pp 303–306. Kluwer Academic Publishers, Dordrecht

    Google Scholar 

  • Bassi R, Pineau B, Dainese P and Marquard J (1993) Carotenoid binding proteins of Photosystem II. Eur J Biochem 212: 297–303

    PubMed  Google Scholar 

  • Berthold DA, Babcock GT and Yocum CF (1981) A highly resolved, oxygen-evolving Photosystem II preparation from spinach thylakoid membranes. FEBS Lett 134: 231–234

    Article  Google Scholar 

  • Brandt WF, deAndrade Rodrigues J and vonHolt C (1988) The amino acid sequence of wheat histone H2B(2). A core histone with a novel repetitive N-terminal extension. Eur J Biochem 173: 547–554

    PubMed  Google Scholar 

  • Burke JJ, Ditto CL and Arntzen CJ (1978) Involvement of the light-harvesting complex in cation regulation of excitation energy distribution in chloroplasts. Arch Biochem Biophys 187: 252–263

    PubMed  Google Scholar 

  • Cheniae GM and Martin IF (1978) Sites of function of manganese within Photosystem II roles in O2 evolution and system II. Biochim Biophys Acta 197: 219–239

    Google Scholar 

  • Dainese P, Høyer-Hansen G and Bassi R (1990) The resolution of chlorophyll a/b binding proteins by a preparative method based on flat bed isoelectric focusing. Photochem Photobiol 51: 693–703

    Google Scholar 

  • Davis DJ and Gross EL (1975) Protein-protein interactions of light-harvesting pigment protein from spinach chloroplasts: I. Ca2+ binding and its relation to protein association. Biochim Biophys Acta 387: 557–567

    PubMed  Google Scholar 

  • Demming B, Winter K, Krüger A and Czygan F-C (1987) Photoinhibition and zeaxanthin formation in intact leaves. Plant Physiol 84: 218–224

    Google Scholar 

  • Demmig-Adams B, Winter K, Krüger A and Czygan F-C (1989) Zeaxanthin and the induction and relaxation kinetics of the dissipation of excess excitation energy in leaves in 2% O2, 0% CO2. Plant Physiol 90: 887–893

    Google Scholar 

  • Droppa M and Horváth G (1990) The role of copper in photosynthesis. Crit Rev Plant Sci 9: 111–123

    Google Scholar 

  • Droppa M, Terry N and Horváth G (1984) Effects of Cu deficiency on photosynthetic electron transport. Proc Natl Acad Sci USA 81: 2369–2373

    Google Scholar 

  • Droppa M, Masojidek J, Rózsa Z, Wolak A, Horváth LI, Farkas T and Horváth G (1987) Characteristics of Cu deficiency-induced inhibition of photosynthetic electron transport in spinach chloroplasts. Biochim Biophys Acta 891: 75–84

    Google Scholar 

  • Ford RC and Evans MCW (1983) Isolation of a Photosystem 2 preparation from higher plants with highly enriched oxygen evolution activity. FEBS Lett 160: 159–164

    Article  Google Scholar 

  • Franzén L-G, Hansson Ö and Andréasson L-E (1985) The roles of the extrinsic subunits in Photosystem II as revealed by EPR. Biochm Biophys Acta 808: 171–179

    Google Scholar 

  • Frieden E (1974) The biochemical evolution of the iron and copper proteins. In: Suttie J and Hoekstra W (eds) TEMA, Vol 2, pp 105–118. University of Wisconsin, Madison

    Google Scholar 

  • Gnevara JJr, Johnson DA, Ramagali LS, Martin BA, Capetillo S and Rodriguez LV (1982) Quantitative aspects of silver deposition in proteins resolved in complex polyacrylamide gels. Electrophoresis 3: 197–205

    Google Scholar 

  • Hager A (1966) Die Zusammengänge zwischen lichtinduzierten Xantohophyll-Umwandlungen und Hill-Reaktion. Berichte der Deutschen botanischen Gesellschaft 79: 94–107

    Google Scholar 

  • Henrysson T, Schröder WP, Spangfort M and Åkerlund H-E (1989) Isolation and characterization of the chlorophyll a/b protein complex CP 29 from spinach. Biochim Biophys Acta 977: 301–308

    Google Scholar 

  • Irrgang K-D, Renger G and Vater J (1991) Isolation, purification and partial characterization of a 30-kDa chlorphyll-a/b-binding protein from spinach. Eur J Biochem 201: 515–522

    PubMed  Google Scholar 

  • Jansson S, Pichersky E, Bassi R, Green BR, Ikeuchi M, Melis A, Simpson DJ, Spangfort M, Staehelin LA and Thornber J (1992) A nomenclature for the genes encoding the chlorophyll a/b-binding proteins of higher plants. Plant Mol Biol Rep 10: 238–250

    Google Scholar 

  • Juhler RK, Andreasson E, Yu S-G and Albertsson P-Å (1993) Composition of photosynthetic pigments in thylakoid membrane vesicles from spinach. Photosynth Res 35: 171–178

    Article  Google Scholar 

  • Karlsson BG, Pascher T, Nordling M, Arvidsson RHA and Lundberg LG (1989) Expression of the blue copper protein azurin from Pseudomonas aeruginosa in Escherichia coli. FEBS Lett 246: 211–217

    Article  PubMed  Google Scholar 

  • Karplus PA, Walsh KA and Herriott JR (1984) Amino acid sequence of spinach ferredoxin: NADP+ oxidoreductase. Biochem 23: 6576–6583

    PubMed  Google Scholar 

  • Kühlbrandt W (1987) Three-dimensional crystals of the light-harvesting chlorophyll a/b protein complex from pea chloroplasts. J Mol Biol 194: 757–762

    PubMed  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685

    PubMed  Google Scholar 

  • Ljungberg U, Åkerlund H-E and Andersson B (1986) Isolation and characterization of the 10-kDa and 22-kDa polypeptides of higher plant Photosystem 2. Eur J Biochem 158: 477–482

    PubMed  Google Scholar 

  • Malmström BG, Andréasson L-E and Reinhammar B (1975) Copper-containing oxidases and superoxide dismutase. In: Boyer PD (ed) The Enzymes, Vol 12, pp 507–579. Academic Press. New York

    Google Scholar 

  • Morishige DT and Thornber JP (1992) Identification and analysis of a barley cDNA clone encoding the 31-kilodalton LHC IIa (CP 29) apoprotein of the light-harvesting antenna complex of Photosystem II. Plant Physiol 98: 238–245

    Google Scholar 

  • Mullet JE and Arntzen CJ (1980) Simulation of grana stacking in a model membrane system: Mediation by a purified light-harvesting pigment-protein complex from chloroplasts. Biochim Biophys Acta 589: 100–117

    PubMed  Google Scholar 

  • Nordling M, Olausson T and Lundberg LG (1990) Expression of spinach plastocyanin in E. coli. FEBS Lett 276: 98–102

    Article  PubMed  Google Scholar 

  • Peisach J and Blumberg WE (1974) Structural implications derived from the analysis of elctron paramagnetic resonance spectra of natural and artificial copper proteins. Arch Biochem Biophys 165: 691–708

    PubMed  Google Scholar 

  • Pichersky E, Subramaniam R, White MJ, Reid J, Aebersold R and Green BE (1991) Chlorophyll a/b binding (CAB) polypeptide of CP 29, the internal chlorophyll a/b complex of PS II: Characterization of the tomato gene encoding the 26 kDa (type I) polypeptide, and evidence for a second CP 29 polypeptide. Mol Gen Genet 227: 277–284

    Article  PubMed  Google Scholar 

  • Scott MG, Crimmins DL, McCourt DW, Tarrand JJ, Eyerman MC and Nahn MH (1988) A simple in situ cyanogen bromide cleavage method to obtain internal amino acid sequence of proteins electroblotted to polyvinylidifluoride membranes. Biochem Biophys Res Commun 155: 1353–1359

    PubMed  Google Scholar 

  • Sibbald PR and Green BR (1987) Copper in Photosystem II: Association with LHC II. Photosynth Res 14: 201–209

    Google Scholar 

  • Takahaski M and Asada K (1986) Sizes of Mn-binding sites in spinach thylakoids. J Biol Chem 261: 16923–16926

    PubMed  Google Scholar 

  • Thayer SS and Björkman O (1990) Leaf xanthophyll content and composition in sun and shade determined by HPLC. Photosynth Res 23: 331–343

    Google Scholar 

  • Vierke G and Struckmeier P (1977) Binding of copper (II) to proteins of the photosynthetic membrane and its correlation with inhibition of electron transport in class II chloroplasts of spinach. Z Naturforsch 32c: 605–610

    Google Scholar 

  • Webber AN and Gray JC (1989) Detection of calcium binding by Photosystem II polypeptides immobilised onto nitrocellulose membrane. FEBS Lett 249: 79–82

    Article  Google Scholar 

  • Yamamoto HY (1985) Xanthophyll cycles. In: Law JH and Rilling HC (eds) methods in Enzymology, Vol 110, pp 303–312. Academic Press, London

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Biochemistry, University of Lund, POB 7007, S-220 07, Lund, Sweden

    Per-Ola Arvidsson, Charlotte Eva Bratt & Hans-Erik Åkerlund

  2. Department of Biochemistry and Biophysics, Chalmers Institute of Technology, S-412 96, Göteborg, Sweden

    Lars-Erik Andréasson

  3. University of Göteborg, S-412 96, Göteborg, Sweden

    Lars-Erik Andréasson

Authors
  1. Per-Ola Arvidsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Charlotte Eva Bratt
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lars-Erik Andréasson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hans-Erik Åkerlund
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arvidsson, PO., Bratt, C.E., Andréasson, LE. et al. The 28 kDa apoprotein of CP 26 in PS II binds copper. Photosynth Res 37, 217–225 (1993). https://doi.org/10.1007/BF00032825

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00032825

Key words

Search

Navigation