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A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and Nod factor induced expression

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Abstract

ENOD12 is one of the first nodulin genes expressed upon inoculation with Rhizobium and also purified Nod factors are able to induce ENOD12 expression. The ENOD12 gene family in pea (Pisum sativum) has two members. A cDNA clone representing PsENOD12A [26] and a PsENOD12B genomic clone [7] have been previously described. The isolation and characterization of a PsENOD12A genomic clone is presented in this paper. By using a Vicia hirsuta-Agrobacterium rhizogenes transformation system it is shown that both genes have a similar expression pattern in transgenic V. hirsuta root nodules. Promoter analyses of both PsENOD12 promoters showed that the 200 bp immediately upstream of the transcription start are sufficient to direct nodule-specific and Nod factor-induced gene expression.

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References

  1. Allison LA, Kiss GB, Bauer P, Poiret M, Pierre M, Savouré A, Kondorosi E, Kondorosi A: Identification of two early nodulin genes with homology to members of the pea ENOD12 gene family. Plant Mol Biol 21: 375–380 (1993).

    PubMed  Google Scholar 

  2. Bauer P, Crespi MD, Szécsi J, Allison LA, Schultze M, Ratet P, Kondorosi E, Kondorosi A: Alfalfa Enod12 genes are differentially regulated during nodule development by Nod factors and Rhizobium in vasion. Plant Physiol 105: 585–592 (1994).

    Article  PubMed  Google Scholar 

  3. Bevan M: Binary Agrobacterium vectors for plant transformation. Nucl Acids Res 12: 8711–8721 (1984).

    PubMed  Google Scholar 

  4. Eckes P, Rosahl S, Schell J, Willmitzer L: Isolation and characterization of a light-inducible, organ-specific gene from potato and analysis of its expression after tagging and transfer into tobacco and potato shoots. Mol Gen Genet 205: 14–22 (1986).

    Google Scholar 

  5. Fisher RF, Long SR: Rhizobium-plant signal exchange. Nature 357: 655–660 (1992).

    Article  PubMed  Google Scholar 

  6. Franssen HJ, Vijn I, Yang WC, Bisseling T: Developmental aspects of the Rhizobium-legume symbiosis. Plant Mol Biol 19: 89–107 (1992).

    Article  PubMed  Google Scholar 

  7. Govers F, Harmsen H, Heidstra R, Michielsen P, Prins M, vanKammen A, Bisseling T: Characterization of the pea ENOD12B gene and expression analyses of the two ENOD12 genes in nodule, stem and flower tissue. Mol Gen Genet 228: 160–166 (1991).

    Article  PubMed  Google Scholar 

  8. Hansen J, Jørgensen J-E, Stougaard J, Marcker KA: Hairy roots—a short cut to transgenic root nodules. Plant Cell Rep 8: 12–15 (1989).

    Google Scholar 

  9. Hirsch AM: Developmental biology of legume nodulation. New Phytol 122: 211–237 (1992).

    Google Scholar 

  10. Hong JC, Nagao RT, Key JL: Characterization and sequence analysis of a developmentally regulated putative cell wall protein gene isolated from soybean. J Biol Chem 262: 8367–8376 (1987).

    PubMed  Google Scholar 

  11. Hong JC, Nasao RT, Key JL: Characterization of a proline-rich cell wall protein gene family of soybean. J Biol Chem 265: 2470–2475 (1990).

    PubMed  Google Scholar 

  12. Horvath B, Heidstra R, Lados M, Moerman M, Spaink HP, Promé J-C, vanKammen A, Bisseling T: Lipooligosaccharides of Rhizobium induce infection related early nodulin gene expression in pea root hairs. Plant J 4: 727–733 (1993).

    Article  PubMed  Google Scholar 

  13. Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).

    PubMed  Google Scholar 

  14. Jørgensen J-E, Stougaard J, Marcker A, Marcker KA: Root nodule specific gene regulation: analysis of the soybean nodulin N23 gene promoter in heterologous symbiotic systems. Nucl Acids Res 16: 39–50 (1988).

    PubMed  Google Scholar 

  15. Jørgensen J-E, Stougaard J, Marker KA: A two-component nodule-specific enhancer in the soybean N23 gene promoter. Plant Cell 3: 819–827 (1991).

    Article  PubMed  Google Scholar 

  16. Journet EP, Pichon M, Dedieu A, deBilly F, Truchet G, Barker DG: Rhizobium meliloti Nod factors elicit cell-specific transcription of the ENOD12 gene in transgenic alfalfa. Plant J 6: 241–249 (1994).

    Article  PubMed  Google Scholar 

  17. Lauridsen P, Franssen H, Stougaard J, Bisseling T, Marcker KA: Conserved regulation of the soybean early nodulin ENOD2 gene promoter in determinate and indeterminate transgenic root nodules. Plant J 3: 483–492 (1993).

    Article  PubMed  Google Scholar 

  18. Lerouge P, Roche P, Faucher C, Maillet F, Truchet G, Promé J-C, Dénarié J: Symbiotic host-specificity of Rhizobium meliloti is determined by a sulphated and acetylated glucosamine oligosaccharide signal. Nature 344: 781–784 (1990).

    Article  PubMed  Google Scholar 

  19. Mattanovich D, Rüker F, daCâmara Machado A, Laimer M, Regner F, Steinkellner H, Himmler G, Katinger H: Efficient transformation of Agrobacterium spp. by electroporation. Nucl Acids Res 17: 6747 (1989).

    PubMed  Google Scholar 

  20. Pichon M, Journet E-P, Dedieu A, deBilly F, Truchet G, Barker D: Rhizobium meliloti elicits transient expression of the early nodulin gene ENOD12 in the differentiating root epidermis of transgenic alfalfa. Plant Cell 4: 1199–1211 (1992).

    Article  PubMed  Google Scholar 

  21. Quandt H-J, Pühler A, Broer I: Transgenic root nodules of Vicia hirsuta. Mol Plant-Microbe Interact 6: 699–706 (1993).

    Google Scholar 

  22. Ramlov K, Laursen NB, Stougaard J, Marcker KA: Sitedirected mutagenesis of the organ-specific element in the soybean leghemoglobin lbc3 gene promoter. Plant J 4: 577–580 (1993).

    Article  PubMed  Google Scholar 

  23. Sambrook J, Frits EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).

    Google Scholar 

  24. Sandal NN, Bojsen K, Marcker KA: A small family of nodule specific genes from soybean. Nucl Acids Res 15: 1507–1519 (1987).

    PubMed  Google Scholar 

  25. Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).

    PubMed  Google Scholar 

  26. Scheres B, van deWiel C, Zalensky A, Horvath B, Spaink H, vanEck H, Zwartkruis F, Wolters AM, Gloudemans T, vanKammen A, Bisseling T: Teh ENOD12 gene product is involved in the infection process during pea-Rhizobium interaction. Cell 60: 281–294 (1990).

    Article  PubMed  Google Scholar 

  27. She Q, Lauridsen P, Stougaard J, Marcker KA: Minimal enhancer elements of the leghemoglobin lba and lbc3 gene promoters from Glycine max L. have different properties. Plant Mol Biol 22: 945–956 (1993).

    Article  PubMed  Google Scholar 

  28. Spaink HP, Sheeley DM, vanBrussel AAN, Glushka J, York WS, Tak T, Geiger O, Kennedy EP, Reinhold VN, Lugtenberg BJJ: A novel highly unsaturated fatty acid moiety of lipo-oligosaccharide signals determines host specificity of Rhizobium. Nature 354: 125–130 (1991).

    PubMed  Google Scholar 

  29. Stougaard J, Sandal NN, Grøn A, Köhle A, Marcker KA: 5′ analysis of the soybean leghemoglobin gene in root nodules of transgenic Lotus corniculatus. EMBO J 6: 3565–3569 (1987).

    Google Scholar 

  30. vanBrussel AAN, Bakhuizen R, vanSpronsen PC, Spaink HP, Tak T, Lugtenberg BJJ, Kijne WJ: Induction of pre-infection thread structures in the leguminous host plant by mitogenic lipo-oligosaccharides of Rhizobium. Science 257: 70–72 (1992).

    Google Scholar 

  31. vanKammen A: Suggested nomenclature for plant genes involved in nodulation and symbiosis. Plant Mol Biol Rep 2: 43–45 (1984).

    Google Scholar 

  32. Vancanneyt G, Schmidt R, O'Connor-Sanchez A, Willmitzer L, Rocha-Sola M: Construction of an introncontaining marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Mol Gen Genet 220: 245–250 (1990).

    Article  PubMed  Google Scholar 

  33. Vijn I, dasNeves L, vanKammen A, Franssen H, Bisseling T: Nod factors and nodulation in plants. Science 260: 1764–1765 (1993).

    PubMed  Google Scholar 

  34. Wilson RC, Long F, Maruoka EM, Cooper JB: A new proline-rich early nodulin from Medicago truncatula is highly expressed in the nodule meristematic cells. Plant Cell 6: 1265–1275 (1994).

    Article  PubMed  Google Scholar 

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Author notes

  1. Igor Kardailsky

    Present address: John Innes Institute, Colney lane, NR47UH, Norwich, England

Authors and Affiliations

  1. Department of Molecular Biology, Agricultural University, Dreijenlaan 3, 6703 HA, Wageningen, Netherlands

    Irma Vijn, Igor Kardailsky, Janneke Drenth, Ab van Kammen & Ton Bisseling

  2. Laboratory of Gene Expression, Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10, DK-8000, Aarhus C, Denmark

    Henning Christiansen, Peter Lauridsen & Erik Østergaard Jensen

  3. Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, D-33501, Bielefeld, Germany

    Hans-Joachim Quandt & Inge Broer

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  1. Irma Vijn
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Vijn, I., Christiansen, H., Lauridsen, P. et al. A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and Nod factor induced expression. Plant Mol Biol 28, 1103–1110 (1995). https://doi.org/10.1007/BF00032670

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  • DOI: https://doi.org/10.1007/BF00032670

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