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The effects of ultraviolet irradiation on P680+ reduction in PS II core complexes measured for individual S-states and during repetitive cycling of the oxygen-evolving complex

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Abstract

Flash-induced absorbance measurements at 830 nm on both nanosecond and microsecond timescales have been used to characterise the effect of ultraviolet light on Photosystem II core particles. A combination of UV-A and UV-B, closely simulating the spectrum of sunlight below 350 nm, was found to have a primary effect on the donor side of P680. Repetitive measurements indicated reductions in the nanosecond components of the absorbance decay with a concomitant appearance and increase in the amplitude of a component with a 10 μs time constant attributed to slow reduction of P680+ by Tyrz when the function of the oxygen evolving complex is inhibited. Single-flash measurements show that the nanosecond components have amplitudes which vary with S-state. Increasing UV irradiation inhibited the amplitude of these components without changing their S-state dependence. In addition, UV irradiation resulted in a reduction in the total amplitude, with no change in the proportion of the 10 μs contribution.

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Abbreviations

BBY-:

PS II membrane fragments

P680-:

primary electron donor of PS II

PS II-:

Photosystem II

QA and QB−:

primary and secondary quinone electron acceptors of PS II

S-state-:

redox state of the oxygenevolving complex

Tyrz−:

tyrosine residue in PS II

UV-A-:

ultraviolet radiation 320–400 nm

UV-B-:

ultraviolet radiation 280–320 nm

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Authors and Affiliations

  1. School of Biological Sciences, A12, University of Sydney, 2006, N.S.W., Australia

    Adele Post & A. W. D. Larkum

  2. School of Physics, A28, University of Sydney, 2006, N.S.W., Australia

    P. B. Lukins & P. J. Walker

Authors
  1. Adele Post
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  2. P. B. Lukins
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  3. P. J. Walker
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  4. A. W. D. Larkum
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Post, A., Lukins, P.B., Walker, P.J. et al. The effects of ultraviolet irradiation on P680+ reduction in PS II core complexes measured for individual S-states and during repetitive cycling of the oxygen-evolving complex. Photosynth Res 49, 21–27 (1996). https://doi.org/10.1007/BF00029424

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  • DOI: https://doi.org/10.1007/BF00029424

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