Abstract
Light-induced chloroplast movements were found to cause changes in chlorophyll fluorescence emission, closely matching those in leaf absorptance, both in terms of the kinetics and the maximum extent of the changes observed in different species. The results demonstrate that chloroplast movements can have a significant effect on the efficiency of light utilization in photosynthesis. They further show that chloroplast movements need to be taken into account in measurements of fluorescence quenching and especially in measurements of light-induced optical changes used to monitor zeaxanthin formation and ΔpH associated light scattering in leaves. Means of minimizing and of adjusting for the influences of chloroplast movements in such measurements are discussed.
Similar content being viewed by others
Abbreviations
- F:
-
fluorescence emission
- PFD:
-
photon flux density
- R:
-
reflectance
- T:
-
transmittance
- α:
-
absorptance
References
Bilger W and Björkman O (1990) Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis. Photosynth Res 25: 173–185
Bilger W and Björkman O (1991) Temperature dependance of violaxanthin de-epoxidation and non-photochemical fluorescence quenching in intact leaves of Gossypium hirsutum L. and Malva parviflora L. Planta 184: 226–234
Bilger W, Björkman O and Thayer SS (1989) Light-induced spectral absorbance changes in relation to photosynthesis and epoxidation state of xanthophyll cycle component in cotton leaves. Plant Physiol 91: 542–551
Björkman O and Powles SB (1981) Leaf movement in the shade species Oxalis oregana. I. Response to light level and light quality. Carnegie Inst. of Washington Year Book 80: 59–62
Britz SJ (1979) Chloroplast and nuclear migration. In: Haupt W and Feinleib ME (eds) Physiology of Movements, Encyclopedia of Plant Physiol, New Series, Vol 7, pp 170–205, Springer-Verlag, Heidelberg
Haupt W and Scheuerlein R (1990) Chloroplast movement. Plant Cell Environ 13: 595–614
Heber U (1969) Conformational changes of chloroplasts induced by illumination of leaves in vivo. Biochim Biophys Acta 180: 302–319
Heber U, Neimanis S and Lange OL (1986) Stomatal aperture, photosynthesis, and water fluxes in mesophyll cells as affected by the abscission of leaves. Simultaneous measurements of gas exchange, light scattering, and chlorophyll fluorescence. Planta 167: 554–562
Inoue Y and Shibata K (1973) Light-induced chloroplast rearrangements and their action spectra as measured by absorption spectrophotometry. Planta 114: 341–358
Inoue Y and Shibata K (1974) Comparative examination of terrestrial plants leaves in terms of light-induced absorption changes due to chloroplast rearrangements. Plant Cell Physiol 15: 717–721
Kobayashi Y, Köster S and Heber U (1982) Light scattering, chlorophyll fluorescence and state of the adenylate system in illuminated spinach leaves. Biochim Biophys Acta 682: 44–54
Kooten Ovan and Snel JFH (1990) The use of chlorophyll fluorescence nomenclature in plant stress physiology. Photosynth Res 25: 147–150
Lechowski Z (1972) Action spectrum of chioroplast displacement in the leaves of land plants. Acta Protozoologica 11: 202–209
Thayer SS and Björkman O (1990) Leaf xanthophyll content and composition in sun and shade determined by HPLC. Photosynth Res 23: 331–343
Weisenseel MH (1968) Vergleichende Untersuchungen zum Einfluß der Temperatur auf lichtinduzierte Chloroplasten-verlagerungen. II. Die statistische Bewegungsgeschwindigkeit der Chloroplasten und ihre Abhängigkeit von der Temperatur. Z Pflanzenphysiol 59: 153–171
Yamamoto HY, Kamite L and Wang Y-Y (1979) An ascorbate-induced absorbance change in chloroplasts from violaxanthin de-epoxidation. Plant Physiol 49: 224–228
Zurzycki J (1962) The action spectrum for the light dependent movements of chloroplasts in Lemna trisulca L. Acta Societatis Botanicorum Poloniae 31: 489–538
Author information
Authors and Affiliations
Additional information
C.I.W.-D.P.B. Publication No. 1116.
Rights and permissions
About this article
Cite this article
Brugnoli, E., Björkman, O. Chloroplast movements in leaves: Influence on chlorophyll fluorescence and measurements of light-induced absorbance changes related to ΔpH and zeaxanthin formation. Photosynth Res 32, 23–35 (1992). https://doi.org/10.1007/BF00028795
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00028795