Abstract
A monoclonal antibody was used to localize abscisic acid in Lavandula stoechas L. plants treated with 1 μM abscisic acid. ABA localization was performed using EDC as the only fixative, which preserves antigenicity and improves the specificity of monoclonal antibodies. A relationship was observed between the effect of abscisic acid on chloroplast ultrastructure and ABA accumulation. Gold particles accumulated on swollen chloroplasts. Our findings provide cytochemical evidence that the chloroplast is a trapping compartment, and yield valuable information on the relation between chloroplast ultrastructure and ABA accumulation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- ABA:
-
abscisic acid
- BSA:
-
bovine serum albumin
- EDC:
-
1-(3-dimethyl-aminopropyl)-3 ethyl carbodiimide
- IgG:
-
immunoglobulin G
- mAB:
-
monoclonal antibodies
- pAB:
-
polyclonal antibodies
References
Bertrand S, Benhamou L, Nadeau P, Dostaler D and Gosselin A (1992) Immunogold localization of free acid abscisic in tomato root cells. Can J Bot 70: 1001–1011
Carlemalm E, Garavito RM and Villiger W (1982) Resin development for electron microscopy and an analysis of embedding at low temperature. J Microsc 126: 123–143
Cowan IR, Raven JA, Hartung W and Farquhar GD (1982) A possible role of abscisic acid in coupling stomatal conductance and photosynthetic carbon metabolism in leaves. Aust J Plant Physiol 9: 484–489
Davies WJ and Jones HG (1991) Abscisic Acid Physiology and Biochemistry, Oxford, Bios Scientific Publishers.
Davies WJ, Metcalfe J, Lodge TA and da Costa AR (1986) Plant growth substances and the regulation of growth under drought. Aust J Plant Physiol 13: 105–125
Eberle J, Wang TL, Cook S, Wells B and Weiler EW (1987) Immunoassay and ultrastructural localization of isopentenylademine and related cytokinins using monoclonal antibodies. Planta 172: 289–297
Hartung W, Heilmann B and Gimmler H (1981) Do chloroplasts play a role in abscisic acid synthesis? Plant Sci Lett 22: 235–242
Hartung W and Slovik S (1991) Physicochemical properties of plant growth regulators and plant tissues determine their distribution and redistribution: stomatal regulation by abscisic acid in leaves. New Phytol 119: 361–382
Herman EM (1988) Immunocytochemical localization of macromolecules with the electron microscope. Ann Rev Plant Physiol 39: 139–155
López-Carbonell M, Alegre L and VanOnckelen HA (1994) Effects of water stress on cellular ultrastructure and on concentrations of endogenous abscisic acid and indole-3-acetic acid in Fatsia japonica leaves. Plant Growth Regul 14: 29–35
López-Carbonell M, Alegre L and Van Onckelen HA (1994) Changes in cell ultrastructure and endogenous abscisic acid and indole-3-acetic acid and concentrations in Fatsia japonica leaves under polyethylene glycol-induced water stress. Plant Growth Regul (in press)
Mertens R, Deus-Neumann B and Weiler EW (1983) Monoclonal antibodies for the detection and quantification of the endogenous plant growth regulator, abscisic acid. FEBS Lett 160: 269–272
Parker ML and Ford MA (1982) The structure of the mesophyll of flag leaves in three Triticum species. Ann Bot 49: 165–176
Petrusz P (1983) Essential requirements for the validity of immunocytochemical staining. J Histochem Cytochem 31: 177–179
Quarrie SA, Whitford PN, Appleford NEJ, Wang TL, Cook SK, Henson IE and Loveys BR (1988) A monoclonal antibody to (S)-abscisic acid: its characterisation and use in radioimmunoassay for measuring abscisic acid in crude extracts of cereal and lupin leaves. Planta 173: 330–339
Raschke K (1987) Action of abscisic acid on guard cells. In: Zeiger E, Farqhuhar GD and Cowan IR (eds) Stomatal Function, pp. 253–279. Stanford Univ. Press, Stanford
Schulze ED (1986) Whole-plant responses to drought. Aust J Plant Physiol 13: 127–141
Slovik S and Hartun W (1992) Compartmental distribution and redistribution of abscisic acid in intact leaves. Planta 187: 37–47
Slovik S, Baier M and Hartung W (1992) Compartmental distribution and redistribution of abscisic acid in intact leaves. I. Mathematical formulation. Planta 187: 14–25
Sossountzov L, Sotta B, Maldiney R, Sabbagh I and Miginiac E (1986) Immunoelectron-microscopy localization of abscisic acid with colloidal gold on Lowicryl-embedded tissues of Chenopodium polyspermum L. Planta 168: 471–481
Sotta B, Sossountzov L, Maldiney R, Sabbagh I, Tachon P and Miginiac E (1985) Abscisic acid localization by light microscopic immunohystochemistry in Chenopodium polyspermum L. J Histochem Cytochem 29: 201–208
Weiler EW (1986) Plant hormone immunoassays based on monoclonal and polyclonal antibodies. In: Linskens HF (ed) Immunology in Plant Sciences. Modern Methods of Plant Analysis, Vol 4, pp. 1–17. Springer, Berlin
Weiler EW, Eberle J, Mertens R, Atzom R, Feyerabend, Jourdan M, Arnscheidt A and Wieczorek U (1986) Monoclonal and polyclonal antibody bassed immunoassay of plant hormones. In: Wang TL (ed) Immunology in Plants Sciences. Modern Methods of Plant Analysis, Vol. 4, pp. 27–58 Cambridge University Press, Cambridge, UK
Welburn FAM and Welburn AR (1973) Influence of gibberellic and abscisic acids and the growth retardant, CCC, upon plastid development. Planta 111: 337–346
Zeevaart JAD and Creelman RA (1988) Metabolism and physiology of abscisic acid. Ann Rev Plant Physiol 39: 439–73
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pastor, A., Cortadellas, N. & Alegre, L. Immunolocalization of abscisic acid by monoclonal antibodies in Lavandula stoechas L. leaves. Plant Growth Regul 16, 287–292 (1995). https://doi.org/10.1007/BF00024788
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00024788