Abstract
Two pectin esterase cDNA clones representing different isozymes with ca. 95% homology were isolated from an early ripening tomato fruit cDNA library. Both clones were longer than previously published sequences, and the encoded proteins possessed extended (229–233 amino acid) putative N-terminal extensions. In addition, the mRNA species corresponding to the two clones showed differential levels of expression in fruit.
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References
Bird CR, Smith CJS, Ray JA, Moureau P, Bevan MW, Bird AS, Hughes S, Morris PC, Grierson D, Schuch W: The tomato polygalacturonase gene and ripening-specific expression in transgenic plants. Plant Mol Biol 11: 651–662 (1984).
Delincée H: Thin-layer focusing of multiple forms of pectinesterase. Phytochemistry 15: 903–906 (1976).
Grierson D, Tucker GA, Keen J, Ray J, Bird CR, Schuch W: Sequencing and identification of a cDNA clone for tomato polygalacturonase. Nucl Acids Res 14: 8595–8603 (1986).
Hall LN, Tucker GA, Smith CJS, Watson CF, Bundick Y, Boniwell JM, Fletcher JD, Ray JA, Schuch W, Bird CR, Grierson D: Antisense inhibition of pectin esterase gene expression in transgenic tomatoes. Plant J 3: 121–129 (1993).
Harriman RW, Tieman DM, Handa AK: Molecular cloning of tomato pectin methylesterase gene and its expression in Rutgers, ripening inhibitor, nonripening, and Never ripe tomato fruits. Plant Physiol 97: 80–87 (1991).
Markovič O, Jörnvall H: Pectinesterase. The primary structure of the tomato enzyme. Eur J Biochem 158: 455–462 (1986).
Markovič O, Jörnvall H: Tomato and Aspergillus niger pectinesterases. Correlation of differences in reports: large species variations. Protein Seq Data Anal 3: 513–515 (1990).
Picton S, Gray J, Barton S, AbuBakar U, Lowe A, Grierson D: cDNA cloning and characterisation of novel ripening-related mRNAs with altered patterns of accumulation in the ripening inhibitor (rin) tomato ripening mutant. Plant Mol Biol, in press (1993).
Pressey R, Avants JK: Multiple forms of pectinesterase in tomatoes. Phytochemistry 11: 3139–3142 (1972).
Pressey R, Avants JK: Solubilization of cell walls by tomato polygalacturonases: effects of pectinesterases. J Food Biochem 6: 57–74 (1982).
Ray J, Knapp J, Grierson D, Bird C, Schuch W: Identification and sequence determination of a cDNA clone for tomato pectin esterase. Eur J Biochem 174: 119–124 (1988).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbour, NY (1989).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Studencki AB, Wallace RB: Allele-specific hybridization using oligonucleotide probes of very high specific activity: discrimination of the human β A- and β B-globin genes. DNA 3: 7–15 (1984).
Tieman DH, Harriman RW, Ramamohan G, Handa AK: An antisense pectin methylesterase gene alters pectin chemistry and soluble solids in tomato fruit. Plant Cell 4: 667–679 (1992).
Tucker GA, Robertson NG, Grierson D: Purification and changes in activity of tomato pectinesterase isoenzymes. J Sci Food Agric 33: 396–400 (1982).
von Heijne G: A new method for predicting signal sequence cleavage sites. Nucl Acids Res 14: 4683–4690 (1986).
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Hall, L.N., Bird, C.R., Picton, S. et al. Molecular characterisation of cDNA clones representing pectin esterase isozymes from tomato. Plant Mol Biol 25, 313–318 (1994). https://doi.org/10.1007/BF00023246
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DOI: https://doi.org/10.1007/BF00023246