Abstract
A method is described for determining the way in which growth rate varies with plant nutrient concentration using a simple nutrient interruption technique incorporating only 2 treatments. The method involves measuring the changes in growth and nutrient composition of otherwise well-nourished plants after the supply of one particular nutrient has been withheld. Critical concentrations are estimated from the relationship between the growth rate (expressed as a fraction of that for control plants of the same size which remained well-nourished throughout) and the concentration of the growth-limiting nutrient in the plants as deficiency developed. Trials of the method using young lettuce plants showed that shoot growth rate was directly proportional to total N (nitrate plus organic N) concentration, and linearly or near-linearly related to K and P concentration over a wide range; the corresponding relationship for nitrate was strongly curvi-linear. Critical concentrations (corresponding to a 10% reduction in growth rate) determined from these results were similar to critical values calculated from models derived from field data, but were generally higher than published estimates of critical concentration (based on reductions in shoot weight) for plants of a similar size. Reasons for these discrepancies are discussed. Nitrate, phosphate or potassium concentrations in sap from individual leaf petioles were highly sensitive to changes in shoot growth rate as deficiency developed, with the slope of the relationships varying with leaf position, due to differences both in their initial concentration and in the rates at which they were utilized in individual leaves. Each nutrient was always depleted more quickly in younger leaves than in older ones, providing earlier evidence of deficiency for diagnostic purposes. Although the plants were capable of accumulating nitrate, phosphate and potassium well in excess of that needed for optimum dry matter production during periods of adequate supply, the rate of mobilization of these reserves was insufficient to prevent reductions in growth rate as the plants became deficient. This brings into question the validity of the conventional concept that luxury consumption provides a store of nutrients which are freely available for use in times of shortage. The implications of these results for the use of plant analysis for assessing plant nutrient status are discussed.
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References
Alt D and Full A-M 1988 Control of the nitrogen status of lettuce by nitrate analysis of plant sap. Acta Hortic. 222, 23–27.
Aslam M, Oaks A and Huffaker R C 1976 Effect of light and glucose on the induction of nitrate reductase and on the distribution of nitrate in etiolated barley leaves. Plant Physiol. 58, 588–591.
Bates T E 1971 Factors affecting critical nutrient concentrations in plants and their evaluation: A review. Soil Sci. 112, 116–130.
Blom-Zandstra M 1989 Nitrate accumulation in vegetables and its relationship to quality. Ann. Appl. Biol. 115, 553–561.
Blom-Zandstra M and Lampe J E M 1983 The effect of chloride and sulphate salts on the nitrate content in lettuce plants (Lactuca sativa L.). J. Plant Nutr. 6, 611–628.
Burns I G 1986 Determination of critical K concentrations in sap from individual leaves or from whole plants using K-interruption experiments. Plant and Soil 94, 301–312.
Burns I G 1987 Effect of nitrate and chloride supply on the growth and nitrate concentration of lettuce. In Proceedings of AFRC Meeting on Plant and Soil Nitrogen Metabolism, Sussex, 7–8 September 1987. Eds. GYates, MMerrick and BSmith. Paper 11, 2 p. AFRC, London, UK.
Burns I G 1988 The use of rapid tests for measurement of plant nutrient status. Anal. Proc. 25, 122–124.
Burns I G and Hutsby W 1984 Development and evaluation of rapid tests for the estimation of phosphate and potassium in plant sap. Commun. Soil Sci. Plant Anal. 15, 1463–1480.
Burns I G and Hutsby W 1986 Cation replacement of potassium in the total shoot of lettuce and its effect on growth during K deficiency. J. Sci. Food Agric. 37, 129–135.
Caporn S J M, Ludwig L J and Flowers T J 1982 Potassium deficiency and photosynthesis in tomato. In Plant Nutrition 1982, Proceedings of the Ninth International Plant Nutrition Colloquium, Warwick, Volume 1. Ed. AScaife. pp 78–83. Commonwealth Agricultural Bureaux, Slough, UK.
Chapman H D 1966 Diagnostic Criteria for Plants and Soils. University of California, Division of Agricultural Sciences, 793 p.
Costigan P A 1985 A survey of nutrient concentrations and early shoot and root growth of drilled lettuce in twenty-one commercial sowings in one year. J. Hortic. Sci. 60, 233–243.
Crooke W M and Simpson W E 1971 Determination of ammonium in Kjeldahl digests of crops by an automated procedure. J. Sci. Food Agri. 22, 9–10.
Ericsson T and Ingestad T 1988 Nutrition and growth of birch seedlings at varied relative phosphorus addition rates. Physiol. Plant. 72, 227–235.
France J and Thornley J H M 1984 Mathematical Models in Agriculture. pp 75–93. Butterworths, London, UK.
Genstat 5 Committee 1987 Genstat 5 Reference Manual. pp 364–369. Clarendon Press, Oxford, UK.
Granstedt R C and Huffaker R C 1982 Identification of the leaf vacuole as a major nitrate storage pool. Plant Physiol. 70, 410–413.
Grant Lipp A E and Goodall D W 1958a Nutrient interactions and deficiency diagnosis in the lettuce. IV. Phosphorus content and response to phosphorus. Aust. J. Biol. Sci. 11, 30–44.
Grant Lipp A E and Goodall D W 1958b Nutrient interactions and deficiency diagnosis in the lettuce. V Potassium content and response to potassium. Aust. J. Biol. Sci. 11, 471–484.
Greenwood D J, Barnes A, Liu K, Hunt J, Cleaver T J and Loquens S M H 1980 Relationships between the critical concentrations of nitrogen, phosphorus and potassium in 17 different vegetable crops and duration of growth. J. Sci. Food Agric. 31, 1343–1353.
Greenwood D J and Draycott A 1989 Experimental validation of an N-response model for widely different crops. Fert. Res. 18, 153–174.
Greenwood E A N, Goodall D W and Titmanis Z V 1965 The measurement of nitrogen deficiency in grass swards. Plant and Soil 23, 97–116.
Hewitt E J 1966 Sand and Water Culture Methods Used in the Study of Plant Nutrition. pp 431–432. Technical Communication No. 22, Commonwealth Bureau of Horticulture and Plantation Crops, Farnham Royal, UK.
Hocking P J 1980 The composition of pholoem exudate and xylem sap from tree tobacco (Nicotiana glauca Grah.). Ann. Bot. 45, 633–643.
Hooymans J J M 1980 A lag phase in vacuolar accumulation of Cl- ions in roots of intact barley plants. Z. Pflanzenphysiol. Bd. 100 S, 185–187.
Hsiao T C and Lauchli A 1986 Role of potassium in plantwater relations. In Advances in Plant Nutrition, Vol 2. Eds. P BTinker and ALauchli. pp 281–382. Praeger, New York.
Hunt J and Seymour D J 1985 Method for measuring nitratenitrogen in vegetables using anion-exchange high-performance liquid chromatography. Analyst 110, 131–133.
Ingestad T 1979 Nitrogen stress in birch seedlings. II. N, K P, Ca and Mg nutrition. Physiol. Plant. 45, 149–157.
Ingestad T 1982 Relative addition rate and external concentration; driving variables used in plant nutrition research. Plant. Cell Environ. 5, 443–453.
Ingestad T and Lund A-B 1979 Nitrogen stress in birch seedlings. I. Growth technique and growth. Physiol. Plant. 45, 137–148.
Leigh R A and Wyn Jones R G 1984 A hypothesis relating critical potassium concentrations for growth to the distribution and functions of this ion in the plant cell. New Phytol. 97, 1–13.
Lemaire G, Gastal F, Cruz P, Greenwood D J and Draycott A 1990 Relationships between plant-N, plant mass and relative growth rate for C3 and C4 crops. In Proceedings of the First Congress of the European Society of Agronomy. Ed. AScaife. Paper 1–005, 2 p. European Society of Agronomy, Colmar, France.
MacKown C T, Volk R J and Jackson W A 1981 Nitrate accumulation, assimilation and transport by decapitated corn roots: Effects of prior nitrate nutrition. Plant Physiol. 68, 133–138.
Macy P 1936 The quantitative mineral nutrient requirements of plants. Plant Physiol. 11, 749–764.
Martinoia E, Heck U and Wiemken A 1981 Vacuoles as storage compartments for nitrate in barley leaves. Nature 289, 292–294.
Mengel K and Arneke W W 1982 Effect of potassium on the water potential, the pressure potential, the osmotic potential and cell elongation in leaves of Phaseolus vulgaris. Physiol. Plant. 54, 402–408.
Pate J S 1975 Exchange of solutes between phloem and xylem and circulation in the whole plant. In Encyclopedia of Plant Physiology, New Series, Vol 1. Eds. M HZimmermann and J AMilburn. pp 451–473. Springer-Verlag, Berlin and New York.
Piper C S 1942 Investigation on copper deficiency in plants. J. Agric. Sci. 32, 143–178.
Reisenauer H M 1978 Soil and Plant-Tissue Testing in California. Bulletin 1879 (Revised), University of California. 54 p.
Reuter D J and Robinson J B 1986 Plant Analysis: An Interpretation Manual. Inkata Press. Melbourne, Australia. 218 p.
RoordavanEysinga J P N L and Smilde K W 1971 Nutritional Disorders in Glasshouse Lettuce. Pudoc. Wageningen. 56 p.
Sanchez C A, Swanson S and Porter P S 1990 Banding P to improve fertilizer use efficiency of lettuce. J. Am. Soc. Hortic. Sci. 115, 581–584.
Scaife A 1988 Derivation of critical concentrations for growth rate from data from field experiments. Plant and Soil 109, 159–169.
Scaife A and Stevens K L 1983 Monitoring sap nitrate in vegetable crops: Comparison of test strips with electrode methods, and effects of time of day and leaf position. Commun. Soil Sci. Plant Anal. 14, 761–771.
Scaife M A and Barnes A 1977 The relationship between crop yield and petiole nitrate concentration at various growth stages. Plant and Soil 46, 705–712.
Slater W G and Goodall D W 1957 Nutrient interactions and deficiency diagnosis in the lettuce. III. Nitrogen content and response to nitrogen. Aust. J. Biol. Sci. 10, 251–278.
Smith F W 1986 Interpretation of plant analysis: Concepts and principles. In Plant Analysis: An Interpretation Manual. Eds. D JReuter and J BRobinson. pp 1–12. Inkata Press, Melbourne, Australia.
Smith R and Scaife M A 1973 The phosphorus requirement of lettuce. I. Use of P intensity estimates to predict the response curve. J. Agric. Sci. Camb. 80, 111–117.
Steenbjerg F 1951 Yield curves and chemical plant analyses. Plant and Soil 3, 97–109.
Ulrich A 1952 Physiological bases for assessing the nutritional requirements of plants. Annu. Rev. Plant Physiol. 3, 207–228.
Wallace A, Abou-Zamzam A M and Mueller R T 1972 Transport of sodium into the xylem exudate of tobacco. Plant Physiol. 50, 388–390.
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Burns, I.G. Influence of plant nutrient concentration on growth rate: Use of a nutrient interruption technique to determine critical concentrations of N, P and K in young plants. Plant Soil 142, 221–233 (1992). https://doi.org/10.1007/BF00010968
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DOI: https://doi.org/10.1007/BF00010968