Photoreaction Mechanisms of Flavoprotein Photoreceptors and Their Applications

Iwata, Tatsuya; Masuda, Shinji

doi:10.1007/978-981-15-8763-4_11

Tatsuya Iwata⁹ &
Shinji Masuda¹⁰

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1293))

5249 Accesses
3 Citations
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Abstract

Three classes of flavoprotein photoreceptors, cryptochromes (CRYs), light-oxygen-voltage (LOV)-domain proteins, and blue light using FAD (BLUF)-domain proteins, have been identified that control various physiological processes in multiple organisms. Accordingly, signaling activities of photoreceptors have been intensively studied and the related mechanisms have been exploited in numerous optogenetic tools. Herein, we summarize the current understanding of photoactivation mechanisms of the flavoprotein photoreceptors and review their applications.

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Abbreviations

As:: Avena sativa
At:: Arabidopsis thaliana
AUREO:: Aureochrome
Bc:: Botrytis cinereal
B-LID:: Blue-light-inducible degradation
BLUF:: Blue light using FAD
BRET:: Bioluminescence resonance energy transfer
bZIP:: Basic leucine zipper
CCT:: C-terminal extension
CIB1:: Cryptochrome-interacting basic-helix-loop-helix 1
CIBN:: CIB N-terminus
cLIPS1:: LOV inhibitor of protein synthesis 1
CRY:: Cryptochrome
dCas9:: Nuclease-dead Cas9
FAD:: Flavin adenine dinucleotide
FGFR1:: Fibroblast growth factor receptor 1
FKF1:: Kelch repeat F-box
FLARE:: Fast light- and activity-regulated expression
FTIR:: Fourier transform infrared
GI:: GIGANTEA
iLID:: Light-induced dimer
LEXY:: Light-inducible nuclear protein export system
LINuS:: Light-induced nuclear localization signal
LINX:: Light-induced-nuclear-export
LOV:: Light-oxygen-voltage
nMag:: Negatively charged Magnet
optoTrk:: Photoactivatable tyrosine receptor kinase
PAC:: Photoactivated adenylyl cyclase
Phot:: Phototropin
Photo-ECM:: Photoswitchable extracellular matrix protein;
PICCORO, PHR:: Photolyase homology region
PixD:: Complex-dependent control
pMag:: Positively charged Magnet
PL:: Photolyase
psd:: Photosensitive degron
REST:: Repressor element 1-silencing transcription factor
Rs:: Rhodobacter sphaeroides
SPARK:: specific protein association tool that gives a transcriptional readout with rapid kinetics
TALEs:: Transcription activator-like effectors;
TEVp:: Tobacco-etch-virus protease
TULIPs:: Tunable light-inducible dimerization tags
VVD:: Vivid coloration
WC1:: White collar1

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Acknowledgments

Research on photoreceptor functions of the authors was partly supported by MEXT/JSPPS KAKENHI Grant Numbers 19H04719 and 19K22418.

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Department of Pharmaceutical Sciences, Toho University, Funabashi, Chiba, Japan
Tatsuya Iwata
Department of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan
Shinji Masuda

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Correspondence to Shinji Masuda .

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The Institute for Solid State Physics, The University of Tokyo, Kashiwa, Japan
Hiromu Yawo
OptoBioTechnology Research Center, Nagoya Institute of Technology, Nagoya, Japan
Hideki Kandori
National Institutes of Natural Sciences, Tokyo, Japan
Amane Koizumi
Institute for Frontier Life and Medical Sciences, Kyoto University, Kyoto, Japan
Ryoichiro Kageyama

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Iwata, T., Masuda, S. (2021). Photoreaction Mechanisms of Flavoprotein Photoreceptors and Their Applications. In: Yawo, H., Kandori, H., Koizumi, A., Kageyama, R. (eds) Optogenetics. Advances in Experimental Medicine and Biology, vol 1293. Springer, Singapore. https://doi.org/10.1007/978-981-15-8763-4_11

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DOI: https://doi.org/10.1007/978-981-15-8763-4_11
Published: 06 January 2021
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