Abstract
Enzymes are widely used nowadays in industrial and household catalysis. Enzyme-catalysed conversions occur in minutes or even in seconds. Further, they catalyse reactions which are difficult to perform by chemical methods, like the enantio- or regioselective hydrolysis or addition of chiral groups. In industries, enzymes are steadily replacing chemical reactions since they are greener and eco-friendly. Enzymes produce fewer by-products, consume less energy, reduce environmental pollution and add improved value to the products. Consequently, it is not surprising to notice the blooming global enzyme market. Major factors driving the market growth of enzymes are cost efficiency, stringent enforcement of environmental regulations in many countries and growing interest among end users. Microbes are an inexhaustible source of enzymes which have numerous advantages with regard to their use in industrial applications, as compared to conventional methods using chemicals. They are good catalysts, increase the rate of reactions and work optimally under given environmental conditions and scaling up of the production process is possible by genetic manipulation. Recombinant DNA technology and protein engineering open up the possibilities of obtaining novel products. Industries utilising microbial enzymes are food, pharmaceutical, detergent, leather, waste management and many others. This chapter discusses the commercial applications of various microbial enzymes and also highlights the sources and nature of industrial applications.
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References
Adrio JL, Demain AL (2014) Microbial enzymes: tools for biotechnological processes. Biomol Ther 4:117–139. https://doi.org/10.3390/biom4010117
Afroz QM, Khan KA, Ahmed P, Uprit S (2015) Enzymes used in dairy industries. Int J Appl Res 1(10):523–527
Al-Abdalall AH, Al-Khaldi EM (2016) Recovery of silver from used X-ray film using alkaline protease from Bacillus subtilis sub sp. subtilis. Afr J Biotechnol 15(26):1413–1416
Ammayappan L (2013) Application of enzyme on woolen products for its value addition: a review. J Text Apparel Technol Manage 8(3):1–12
Amri E, Mamboya F (2012) Papain, a plant enzyme of biological importance: a review. Am J Biochem Biotechnol 8(2):99–104. https://doi.org/10.3844/ajbbsp.2012.99.104
Andualema B, Gessesse A (2012) Microbial lipases and their industrial applications. Biotechnology 11(3):100–118
Araujo R, Casal M, Cavaco-Paulo A (2008) Application of enzymes for textile fibres processing. Biocatal Biotransformation 26(5):332–349
Aravindan R, Anbumathi P, Viruthagiri T (2007) Lipase applications in food industry. Indian J Biotechnol 6:141–158
Aravindhan R, Monika V, Balamurugan K, Subramanian V, Rao JR, Thanikaivelan P (2017) Highly clean and efficient enzymatic dehairing in green solvents. J Clean Prod 140:1578–1586
Arshad ZIM, Amid A, Yusof F, Jaswir I, Ahmad K, Loke SP (2014) Bromelain: an overview of industrial application and purification strategies. Appl Microbiol Biotechnol 98:7283–7297. https://doi.org/10.1007/s00253-014-5889-y
Atanasova J, Moncheva P, Ivanova I (2014) Proteolytic and antimicrobial activity of lactic acid bacteria grown in goat milk. Biotechnol Biotechnol Equip 28(6):1073–1078
Bajpai PK (2010) Solving the problems of recycled fiber processing with enzymes. Bioresources 5(2):1311–1325
Behera BC, Sethi BK, Mishra RR, Dutta SK, Thatoi HN (2017) Microbial cellulases—diversity & biotechnology with reference to mangrove environment: a review. J Genet Eng Biotechnol 15:197–210
Chanalia P, Gandhi D, Singh JD (2011) Applications of microbial proteases in pharmaceutical industry: an over view. Rev Med Microbial 22(4):96–101
Chauhan PS, Puri N, Sharma P, Gupta N (2012) Mannanases: microbial sources, production, properties and potential biotechnological applications. Appl Microbiol Biotechnol 93:1817–1830. https://doi.org/10.1007/s00253-012-3887-5
Cho SA, Cho JC, Han SH (2007) Cosmetic composition containing enzyme and amino acid. Amorepacific Corporation (11/990):431
Choudhary RB, Jana AK, Jha MK (2004) Enzyme technology applications in leather processing. Indian J Chem Technol 11:659–671
Craik CS, Page MJ, Madison EL (2011) Proteases as therapeutics. Biochem J 435(1):1–16. https://doi.org/10.1042/BJ20100965
Dahiya S (2016) Industrial applications of phytases. Int J Appl Res 2(2):95–98
de Souza PM, Magalhaes PO (2010) Application of microbial alpha-amylases in industry: a review. Braz J Microbiol 41:850. https://doi.org/10.1590/S1517-83822010000400004
Ducros V, Czizek V, Belaich A, Gaudin C, Fierobe HP, Belaich JP, Davies GJ, Haser R (1995) Crystal structure of the catalytic domain of cellulose. Structure 3(9):939–949
Dutt D, Tyagi CH, Singh RP, Kumar A (2012) Effect of enzyme concoctions on fiber surface roughness and deinking efficiency of sorted office paper. Cellul Chem Technol 46:611–623
Freddi G, Mossotti R, Innocenti R (2003) Degumming of silk fabrics with several proteases. J Biotechnol 106:101–112
Golan AE (2011) Cellulase: types and action, mechanism and uses; biotechnology in agriculture, industry and medicine. Nova Science, New York, p c2011
Gopinath SC, Anbu P, Arshad MK, Lakshmipriya T, Voon CH, Hashim U, Chinni SV (2017) Biotechnological processes in microbial amylase production. Biomed Res Int 1272193:9. https://doi.org/10.1155/2017/1272193
Guo JS, Xu YF (2011) Review of enzymatic sludge hydrolysis. J Bioremed Biodegr 2:130. https://doi.org/10.4172/2155-6199.1000130
Gupta R, Beg QK, Lorenz P (2002) Bacterial alkaline proteases: molecular approaches and industrial applications. Appl Microbiol Biotechnol 59:15–32. https://doi.org/10.1007/s00253-002-0975-y
Gupta R, Gigas P, Mohapatra H, Goswani VK, Chauhan B (2003) Microbial alpha-amylases: a biotechnological perspective. Process Biochem 38:1599–1616
Hasan F, Sha AA, Hameed A (2006) Industrial applications of microbial lipases. Enzyme Microb Technol 39:235–251
Heredia-Sandoval NG, Valencia-Tapia MY, de la Barca AMC, Islas-Rubio AR (2016) Microbial proteases in baked goods: modification of gluten and effects on immunogenicity and product quality. Foods 5:59. https://doi.org/10.3390/foods5030059
Ismail B, Nielsen SS (2010) Invited review: plasmin protease in milk: current knowledge and relevance to dairy industry. Dairy Sci 93:4999–5009. https://doi.org/10.3168/jds.2010-3122
Jegannathan KR, Nielson PH (2012) Environmental assessment of enzyme use in industrial production—a literature review. J Clean Prod 42:228–240
Jisha VN, Smitha RB, Pradeep S, Sreedevi S, Unni KN, Sajith S, Priji P, Josh MS, Benjamin S (2013) Versatility of microbial proteases. Adv Enzyme Res 1:39–51. https://doi.org/10.4236/aer.2013.13005
Karmakar M, Ray RR (2011) Current trends in research and application of microbial Cellulases. Res J Microbiol 6(1):41–53
Kavitha M (2016) Cold active lipases—an update. Front Life Sci 9:226. https://doi.org/10.1080/21553769.2016.1209134
Kiran S, Arshad Z, Nosheen S, Kamal S, Gulzar T, Majeed MS, Jannat M, Rafique MA (2016) Microbial lipases: production and applications: a review. J Biochem Biotechnol Biomater 1(2):7–20
Kuhad RC, Gupta R, Singh A (2011) Microbial Cellulases and their industrial applications. Enzyme Res 2011:280696. https://doi.org/10.4061/2011/280696
Kumar D, Savitri, Thakur N, Verma R, Bhalla TC (2008) Microbial proteases and applications as laundry detergent additive. Res J Microbiol 3(12):661–672
Kumar D, Kumar SS, Kumar J, Kumar O, Mishra SV, Kumar R, Malyan SK (2017) Xylanases and their industrial applications: a review. Biochem Cell Arch 17(1):353–360
Kyaw N, de Mesquita RF, Kameda E, Neto JC, Langone MA (2010) Characterization of commercial amylases for the removal of filter cake on petroleum wells. Appl Biochem Biotechnol 161:171–180. https://doi.org/10.1007/s12010-009-8773-y
Lei XG, Porres JM, Mullaney EJ, Brinch-Pedersen H (2007) Phytase: source, structure and application. In: Polaina J, MacCabe AP (eds) Industrial enzymes. Springer, Dordrecht, pp 505–529. https://doi.org/10.1007/1-4020-5377-0_29
Li Q, Yi L, Marek P, Iverson BL (2013) Commercial proteases: present and future. FEBS Lett 587:1155–1163
Lopez-Otín C, Bond JS (2008) Proteases: multifunctional enzymes in life and disease. J Biol Chem 283:30433–30437
Mane P, Tale V (2015) Overview of microbial therapeutic enzymes. Int J Curr Microbiol App Sci 4(4):17–26
Mathias TRS, Paula A, de Almeida e Silva JB, de Mello PPM, Eliana S (2017) Brewery waste reuse for protease production by lactic acid fermentation. Food Technol Biotechnol 55:218–224. https://doi.org/10.17113/tb.55.02.17.4378
Mienda BS, Yahya A, Galadima IA, Shamsir MS (2014) An overview of microbial proteases for industrial applications. Res J Pharm Biol Chem Sci 5(1):388–396
Miguel ASM, Martins-Meyer TS, da Costa Figueiredo EV, Lobo BWP, Dellamora-Ortizet GM (2013) Enzymes in bakery: current and future trends. Food Industry Innocenzo Muzzalupo, Intech Open. https://doi.org/10.5772/53168
Mojsov K (2011) Application of enzymes in the textile industry: a review. In: II International congress “Engineering, ecology and materials in the processing industry”, Bosnia and Herzegovina
Mojsov K (2012) Microbial alpha-amylases and their industrial applications: a review. Int J Manage IT Eng 2(9):272–295
Momsia T, Momsia P (2013) A review on microbial lipase-versatile tool for industrial applications. Int J Life Sci Biotechnol Pharma Res 2(4):1–16
Mótyán JA, Tóth MF, Tőzsér J (2013) Research applications of proteolytic enzymes in molecular biology. Biomol Ther 3:923–942. https://doi.org/10.3390/biom3040923
Pandi A, Ramalingam S, Rao JR, Kamini NR, Gowthaman MK (2016) Inexpensive alpha amylase production and application for fiber splitting in leather processing. Royal Soc Chem 6:33170. https://doi.org/10.1039/c5ra26373e.
Parpalliwar JP, Patil PS, Patil ID, Deshannavar UB (2015) Extraction of silver from waste X-ray films using protease enzyme. Int J Adv Biotechnol Res 6(2):220–226
Pedrolli DB, Monteiro AC, Gomes E, Carmona EC (2009) Pectin and pectinases: production, characterization and industrial application of microbial pectinolytic enzymes. Open Biotechnol J 3:9–18
Pogaku P, Bhavani P, Manjusha T, Saritha K (2017) Lipases in environmental management. Int J Curr Res Aca Rev 5(4):106–109. https://doi.org/10.20546/ijcrar.2017.504.016
Porta R, Pandey A, Rosell CM (2010) Enzymes as additives or processing aids in food biotechnology. Enzyme Res 2010:436859. https://doi.org/10.4061/2010/436859
Prakasan P, Sreedharan S, Faisal PA, Benjamin S (2016) Microbial lipases-properties and applications. J Microbiol Biotechnol Food Sci 6(2):799–807. https://doi.org/10.15414/jmbfs.2016.6.2.799-807
Ramnath L, Sithole B, Govinden R (2017) Classification of lipolytic enzymes and their biotechnological applications in the pulping industry. Can J Microbiol 63:179–192. https://doi.org/10.1139/cjm-2016-0447
Rana N, Walia A, Gaur A (2013) Alpha amylases from microbial sources and its potential applications in various industries. Natl Acad Sci Lett 36:9–17. https://doi.org/10.1007/s40009-012-0104-0
Rawlings ND, Barrett AJ, Bateman A (2011) Asparagine peptide lyases, a seventh catalytic type of proteolytic enzymes. J Biol Chem 286:38321–38328. https://doi.org/10.1074/jbc.M111.260026
Roman HJ, Burgess JE, Pletschk BI (2006) Enzyme treatment to decrease solids and improve digestion of primary sewage sludge. Afr J Biotechnol 5(10):963–967
Saini R, Saini HS, Dahiya A (2017) Amylases: characteristics and industrial applications. J Pharmacogn Phytochem 6(4):1865–1871
Sajith S, Priji P, Sreedevi S, Benjamin S (2016) An overview on fungal cellulases with an industrial perspective. J Nutr Food Sci 6:461. https://doi.org/10.4172/2155-9600.1000461
Salihu A, Alam MZ (2012) Production and applications of microbial lipases: a review. Sci Res Essays 7(30):2667–2677. https://doi.org/10.5897/SRE11.2023.
Salleh AB, Rahman RNZRA, Basri M (2006) New lipases and proteases. Nova Science Publishers, Inc., Hauppauge, NY. ISBN: 978-1-60876-518-8
Sarrouh B, Santos TM, Miyoshi A, Dias R, Azevedo V (2012) Up-to-date insight on industrial enzymes applications and global market. J Bioprocess Biotechniq 10:4–002 https://doi.org/10.4172/2155-9821.S4-002
Sawant R, Nagendran S (2014) Protease: an enzyme with multiple industrial applications. World J Pharm Pharm Sci 3:568–579
Shah SR (2013) Chemistry and applications of cellulase in textile wet processing. Res J Eng Sci 2(7):1–5
Shankar S, More SV, Laxman RS (2010) Recovery of silver from waste X-ray film by alkaline protease from Conidiobolus coronatus. Kathmandu Univ J Sci Eng Technol 6(1):60–69
Sharma N, Rathore M, Sharma M (2012) Microbial pectinase: sources, characterisation and applications. Rev Environ Sci Biotechnol 12(1):45–60. https://doi.org/10.1007/s11157-012-9276-9
Shelatkar T, Padalia U (2016) Lipase: an overview and its industrial applications; international journal of engineering science and. Computing 6(10):2629–2631
Singh R, Kumar M, Mittal A, Mehta PK (2016a) Microbial cellulases in industrial applications. Ann Appl Biosci 3(4):2016
Singh R, Kumar M, Mittal A, Mehta PK (2016b) Microbial enzymes: industrial progress in 21st century. 3 Biotech 6:174. https://doi.org/10.1007/s13205-016-0485-8
Singh R, Mittal A, Kumar M, Mehta PK (2016c) Amylases: a note on current applications. Int Res J 5(11):27–32
Singh R, Mittal A, Kumar M, Mehta PK (2016d) Microbial proteases in commercial applications. J Pharm Chem Biol Sci 4(3):365–374
Sivaramakrishnan S, Gangadharan D, Nampoothiri KM, Soccol CR, Pandey A (2006) Alpha amylases from microbial sources- an overview on recent developments. Food Technol Biotechnol 44(2):173–184
Sukumaran RK, Singhania RR, Pandey A (2005) Microbial cellulase- production, applications and challenges. J Sci Ind Res 64:832–844
Sumantha A, Larroche C, Pandey A (2006) Microbiology and industrial biotechnology of food-grade proteases: a perspective. Food Technol Biotechnol 44(2):211–220
Sundarram A, Murthy TPK (2014) Alpha amylase production and applications: a review. J Appl Environ Microbiol 2:166–175
Taniguchi H, Honnada Y (2009) Amylases. Elsevier Inc.
Tapre AR, Jain RK (2014) Pectinases: enzymes for fruit processing industry. Int Food Res J 21(2):447–453
Ugo AK, Amara AV, Igwe CN, Kenechuwku U (2017) Microbial lipases: a prospect for biotechnological industrial catalysis for green products: a review. Ferment Technol 6(2):144. https://doi.org/10.4172/2167-7972.1000144.
Verma A, Singh H, Anwar S, Chattopadhyay A, Tiwari KK, Kaur S, Dhilon GS (2016) Microbial keratinases: industrial enzymes with waste management potential. Crit Rev Biotechnol 37(4):476–491. https://doi.org/10.1080/07388551.2016.1185388
Walker TJ, Dayan SH (2014) Comparison and overview of currently available neurotoxins. J Clin Aesthet Dermatol 7(2):31–39
Zhang XZ, Zhang YHP (2013) Cellulases: characteristics, sources, production and applications. John Wiley & Sons, Inc., Hoboken, NJ
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Ramesh, A., Harani Devi, P., Chattopadhyay, S., Kavitha, M. (2020). Commercial Applications of Microbial Enzymes. In: Arora, N., Mishra, J., Mishra, V. (eds) Microbial Enzymes: Roles and Applications in Industries. Microorganisms for Sustainability, vol 11. Springer, Singapore. https://doi.org/10.1007/978-981-15-1710-5_6
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