Abstract
Starch, cellulose, and hemicellulose are promising renewable feedstock from terrestrial plants for biofuel production. Cell surface engineering was applied to the construction of whole-cell biocatalyst for the direct production of ethanol from these polysaccharides by displaying enzymes on yeast cell surface. The environmental polysaccharides can be efficiently degraded into monosaccharides by the synergistic effects between the displayed enzymes. The generated monosaccharides are quickly incorporated into the cells and assimilated into ethanol by intracellular metabolic activities. In this chapter, ethanol production from starch, cellulose, and hemicellulose by surface-engineered yeasts is introduced, and the advantages of cell surface display of enzymes, such as their suitability for consolidated bioprocessing (CBP), are described.
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Kuroda, K. (2019). Energy Production: Biomass – Starch, Cellulose, and Hemicellulose. In: Ueda, M. (eds) Yeast Cell Surface Engineering. Springer, Singapore. https://doi.org/10.1007/978-981-13-5868-5_2
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DOI: https://doi.org/10.1007/978-981-13-5868-5_2
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