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Isothermal Titration Calorimetry: A Powerful Tool for the Characterization of Molecular Interactions

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Thermodynamics and Biophysics of Biomedical Nanosystems

Part of the book series: Series in BioEngineering ((SERBIOENG))

Abstract

Isothermal titration calorimetry (ITC) is a well-established technique that allows the accurate and precise determination of binding equilibrium constants. It is able to provide detailed thermodynamic description of reacting systems without the need for van’t Hoff analysis. ITC plays an important role in biology, biochemistry and medicinal chemistry, providing researchers with important information on the structure, stability and functionality of biological and synthetic molecules. This review demonstrates the power and versatility of ITC in providing accurate, rapid, and label-free measurement of the thermodynamics of molecular interactions. Moreover, this work focuses on recent studies employing ITC to investigate compounds of great biotechnological interest.

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Authors and Affiliations

  1. Biomolecular Physics Laboratory, INRaSTES, National Centre for Scientific Research ‘Demokritos’, Athens, Greece

    Angelos Thanassoulas & George Nounesis

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  1. Angelos Thanassoulas
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  2. George Nounesis
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Correspondence to Angelos Thanassoulas .

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  1. School of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece

    Costas Demetzos

  2. School of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece

    Natassa Pippa

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Thanassoulas, A., Nounesis, G. (2019). Isothermal Titration Calorimetry: A Powerful Tool for the Characterization of Molecular Interactions. In: Demetzos, C., Pippa, N. (eds) Thermodynamics and Biophysics of Biomedical Nanosystems. Series in BioEngineering. Springer, Singapore. https://doi.org/10.1007/978-981-13-0989-2_4

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  • DOI: https://doi.org/10.1007/978-981-13-0989-2_4

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