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Overexpression of the Endo-inulinase Gene from Two Different Sources and Characteristics Analysis

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Advances in Applied Biotechnology (ICAB 2016)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 444))

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Abstract

The endo-inulinase genes from Aspergillus niger (A. niger) TCCC41064 and Penicillium sp. TN-88 were inserted into the expression vector pPIC9 K. The recombinant plasmids were subsequently transformed into P. pastoris GS115 by electroporation, respectively. The two recombinants (P. pastoris GS115/pPIC9 K-inuA, GS115/pPIC9 K-inuC) were purified and characterized. The molecular weight of the purified inuA and inuC were both about 66.2 kDa. The specific activity of transformant endo-inulinase inuA and inuC were 13.5 and 69.3 U/mL. The optimal pH and temperature were 5.0, 60 °C for inuA, and 4.0, 50 °C, respectively. InuA, 83.2% of the residual enzymatic activity at 55 °C for 9 h. Meanwhile, inuC retained 75.4% at 40 °C for 9 h. The purified inuC converts inulin into trisaccharide (74.8%) and for the inuA was 36.7%. The researches on inulinase will provide a better engineering strain for designing the industrial biocatalyst.

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Authors and Affiliations

  1. Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China

    Li-Kun Wei, Qing-Long Xin, Zhi-Mei Feng, Xue-Yan Xing, Wei Feng & Yu Li

Authors
  1. Li-Kun Wei
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  2. Qing-Long Xin
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  3. Zhi-Mei Feng
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  4. Xue-Yan Xing
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  5. Wei Feng
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  6. Yu Li
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Correspondence to Yu Li .

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Editors and Affiliations

  1. College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China

    Hao Liu

  2. College of Life Sciences, Nankai University, Tianjin, China

    Cunjiang Song

  3. Institute of Biology Leiden, Leiden University, Leiden, The Netherlands

    Arthur Ram

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© 2018 Springer Nature Singapore Pte Ltd.

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Wei, LK., Xin, QL., Feng, ZM., Xing, XY., Feng, W., Li, Y. (2018). Overexpression of the Endo-inulinase Gene from Two Different Sources and Characteristics Analysis. In: Liu, H., Song, C., Ram, A. (eds) Advances in Applied Biotechnology. ICAB 2016. Lecture Notes in Electrical Engineering, vol 444. Springer, Singapore. https://doi.org/10.1007/978-981-10-4801-2_65

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