Abstract
Heavy metals are natural constituents of the earth’s crust. There is a significant alteration in the geochemical cycles and biological balance of these heavy metals due to various anthropogenic activities. These anthropogenic activities result in the release of bioavailable forms of various heavy metals such as mercury, lead, cadmium, nickel, copper, zinc, etc. into soil and aquatic environments. Prolonged exposures to these heavy elements lead to harmful health implications on different domains of terrestrial and aquatic life. Due to several limitations associated with physical and chemical methods for remediation of contaminated sites, bioremediation has been explored these days as an alternate technology for treatment of heavy metal pollution in soil and water. Various microorganisms such as bacteria and fungi along with plants play a vital role in biotransformation of these heavy metals into nontoxic forms, through processes such as bioremediation and phytoremediation, respectively. Recent progress in genetics has provided the driving force toward the use of engineering improved microbes and enzymes for bioremediation. Keeping these future remediation tolls in mind, present review investigated the abilities of wild microorganisms and plants in terms of tolerance and biotransformation of heavy metals along with their genetically engineered counterparts to explore these immense and valuable biological resources for bioremediation.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Adriano DC (2001) Trace elements in terrestrial environments: biogeochemistry bioavailability and risks of metals, 3rd edn. Springer, New York
Baker AJM, Reeves RD, McGrath SP (1991) In situ decontamination of heavy metal polluted soils using crops of metal accumulating plants: a feasibility study. In: Hinchee RE, Olfenbuttel RF (eds) Bioremediation, pp 539–544
Barkay T, Turner R, Saouter E, Horn J (1992) Mercury bio-transformations and their potential for remediation of mercury contamination. Biodegradation 3:147–159
Barkay T, Miller SM, Summers AO (2003) Bacterial mercury resistance: from atoms to ecosystems. FEMS Microbiol Rev 27:355–384
Bentley R, Chasteen TG (2002) Microbial methylation of metalloids: arsenic, antimony, and bismuth. Microbiol Mol Biol Rev 66:250–271
Bittsanszkya A, Kömives T, Gullner G, Gyulai G et al (2005) Ability of transgenic poplars with elevated glutathione content to tolerate zinc(2+) stress. Environ Int 31:251–254
Bizily SP, Rugh CL, Meagher RB (2000) Phytodetoxification of hazardous organomercurials by genetically engineered plants. Nat Biotechnol 18:213–217
Brim H, McFarlan SC, Fredrickson JK, Minton KW (2000) Engineering Deinococcus radiodurans for metal remediation in radioactive mixed waste environments. Nat Biotechnol 18:85–90
Brodkin E, Copes R, Mattman A, Kennedy J (2007) Lead and mercury exposures: interpretation and action. Can Med Assoc J 176:59–63
Chaney RL (1983) Plant uptake of inorganic waste constitutes. In: Land treatment of hazardous wastes. Park Ridge Noyes data corp, London, pp 50–76
Che D, Meagher RB, Heaton ACP, Lima A (2003) Expression of mercuric ion reductase in eastern cottonwood (Populus deltoids) confers mercuric ion reduction and resistance. Plant Biotechnol J 1:311–319
Chen M, Ma LQ, Singh SP, Cao RX et al (2003) Field demonstration of in situ immobilization of soil Pb using P amendment. Adv Environ Res 8:93–102
Cho DH, Yoo MH, Kim EY (2004) Biosorption of lead (Pb2+) from aqueous solution by Rhodotorula aurantiaca. J Microbiol Biotechnol 14:250–255
Chowdhury S, Bala NN, Dhauria P (2012) Bioremediation – a natural way for cleaner environment. Int J Pharm Chem Biol Sci 2:600–611
Cunningham DP, Lundie LL (1993) Precipitation of cadmium by Clostridium thernoaceticum. Appl Environ Microbiol 59:7–14
Danika L, Norman TL (2005) Phytoremediation of toxic trace elements in soil and water. J Ind Microbiol Biotechnol 32:514–520
Dash HR, Das S (2012) Bioremediation of mercury and the importance of bacterial mer genes. Int Biodeterior Biodegrad 75:207–213
Davison J (2005) Risk mitigation of genetically modified bacteria and plants designed for bioremediation. J Ind Microbiol Biotechnol 32:639–650
Deng X, Yi XE, Liu G (2007) Cadmium removal from aqueous solution by gene modified Escherichia coli JM109. J Hazard Mater 139:340–344
Dhankher OP, Li Y, Rosen BP, Shi J et al (2002) Engineering tolerance and hyper-accumulation of arsenic in plants by combining arsenate reductase and g-glutamylcysteine synthetase expression. Nat Biotechnol 20:1140–1145
Doty SL (2007) Enhanced metabolism of halogenated hydrocarbons in transgenic plants contain mammalian P450 2E1. Proc Natl Acad Sci U S A 97:6287–6291
Drewniak L, Dziewit L, Ciezkowska M, Gawor J et al (2013) Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance. J. Biotechnol 164:479–488
Eapen S, D’Souza SF (2005) Prospects of genetic engineering of plants for phytoremediation of toxic metals. Biotechnol Adv 23:97–114
Eisler R (2004) Arsenic hazards to humans, plants, and animals from gold mining. Rev Environ Contam Toxicol 180:133–165
EPA (2007) Treatment Technologies for Site Cleanup: Annual Status Report; United States Environmental Protection Agency (EPA): Washington, DC, USA
EPA (2008) Mercury human exposure. US Environmental Protection Agency
Ferner DJ (2001) Toxicity, heavy metals. Emerg Med J 2:1
Flathman PE, Lanza GR (1998) Phytoremediation: current views on an emerging green technology. J Soil Contam 7:415–432
Flora SJS (2002) Lead exposure: health effects, prevention and treatment. J Environ Biol 23:25–41
Fowler BA (1998) Role of lead binding proteins in mediating lead bioavailability. Environ Health Perspect 106:1585–1587
Gadd GM (2000) Bioremedial potential of microbial mechanisms of metal mobilization and immobilization. Curr Opin Biotechnol 11:271–279
Gan S, Lau EV, Ng HK (2009) Remediation of soils contaminated with polycyclic aromatic hydrocarbons (PAHs). J Hazard Mater 172:532–549
Garbarino JR, Hayes H, Roth D et al (2005) Contaminants in the Mississippi river. U. S. Geological Survey Circular, Virginia, U.S.A. 1133
Garbisu C, Alkorta I (2003) Basic concepts on heavy metal soil bioremediation. Eur J Min Porcess Environ Prt 3:58–66
Giller KE, Witter E, McGrath SP (1998) Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils: a review. Soil Biol Biochem 30:1389–1414
Gisbert C, Ros R, Haro AD, Walker DJ (2003) A plant genetically modified that accumulates Pb is especially promising for phytoremediation. Biochem Biophys Res Commun 2:440–445
Graz M, Pawlikowska-Pawlęga B, Jarosz-Wilkołazka A, (2011) Growth inhibition and intracellular distribution of Pb ions by the white-rot fungus Abortiporus biennis. Int. Biodeter. Biodegr 65:124–129
Gullner G (2001) Enhanced tolerance of transgenic poplar plants over-expressing gamma-glutamylcysteine synthetase towards chloroacetanilide herbicides. J Exp Bot 52:971–979
Guo J, Dai X, Xu W, Ma M (2008) Over-expressing GSHI and AsPCSI simultaneously increase the tolerance and accumulation of cadmium and arsenic in Arabidopsis thaliana. Chemosphere 72:1020–1026
Halim CE, Scott JA, Amal R, Short SA et al (2005) Evaluating the applicability of regulatory leaching tests for assessing the hazards of Pb-contaminated soils. J Hazard Mater 120:101–111
Holmes P, James KAF, Levy LS (2009) Is low-level environmental mercury exposure of concern to human health? Sci Total Environ 408:171–182
Huang CC, Chen MW, Hsieh JL, Lin WH et al (2006a) Expression of mercuric reductase from Bacillus megaterium MB1 in eukaryotic microalga Chlorella sp. DT: an approach for mercury phytoremediation. Appl Microbiol Biotechnol 72:197–205
Huang D, Zeng G, Jiang X, Feng C et al (2006b) Bioremediation of Pb-contaminated soil by incubating with Phanerochaete chrysosporium and straw. J Hazard Mater 134:268–276
Hussein H, Krull R, Abou El-Ela SI, Hempel DC (2001) Interaction of the different heavy metal ions with immobilized bacterial culture degrading xenobiotic wastewater compounds. In: Proceedings of the Second International Water Association World Water Conference, Berlin, Germany. pp. 15–19
IARC (International Agency for Research on Cancer) (1993) Cadmium and certain cadmium compounds. In: IARC monographs on the evaluation of the carcinogenic risk of chemicals to humans. Beryllium, cadmium, mercury and exposures in the glass manufacturing industry. IARC monograph 58. Lyon, France: World Health Organization. International Agency for Research on Cancer, 119–237
Jackson WJ (1982) Summers AO: biochemical characterization of HgCl2- inducible polypeptides encoded by the mer operon of plasmid R 100. J Bacteriol 151:962–970
Johansson M (2002) A review of risks associated to arsenic, cadmium, lead, mercury and zinc. The market implication of integrated management for heavy metals flows for bioenergy use in the European Union, Kalmar University, Department of biology and environmental Science, Sweden. p115
Kang SH, Singh S, Kim YJ et al (2007) Bacteria metabolically engineered for enhanced phytochelatin production and cadmium accumulation. Appl Environ Microbiol 73(19):6317–6320
Kim SK, Lee BS, Wilson DB, Kim EK (2005) Selective cadmium accumulation using recombinant Escherichia coli. J Biosci Bioeng 99:109–114
Koplan JP (1999) Toxicological profile for cadmium, published by ATSDR, U.S. Department of health and human services p 439
Kumar A, Cameotra SS, Gupta S (2012) Screening and characterization of potential cadmium biosorbent Alcaligenes strain from industrial effluent. J Basic Microbiol 52:160–166
Laddaga RA, Chu L, Misra TK, Silver S (1987) Nucleotide sequence and expression of the mercurial-resistance operon from Staphylococcus aureus plasmid pI258. Proc Natl Acad Sci 84:5106–5110
Lam TV, Agovino P, Niu X, Roche L (2007) Linkage study of cancer risk among lead exposed workers in New Jersey. Sci Total Environ 372:455–462
Lasat MM (2002) Phytoextraction of toxic metals: a review of biological mechanisms. J Environ Qual 31:109–120
Lenntech R (2004) Lenntech Water Treatment and Air Purification. Water Treatment. (http://www.excelwater.com/thp/filters/Water-Purification.htm)
Leonard A (1991) Arsenic. In: Merian E (ed) Mineral and their compounds in the environment: occurrence, analysis and biological relevance, 2nd ed. VCH, Weinheim, pp 751–773
Li P, Feng XB, Qiu GL, Shang LH (2009) Mercury pollution in Asia: a review of the contaminated sites. J Mater 168:591–601
Lim PE, Mak KY, Mohamed N, Noor AM (2003) Removal and speciation of heavy metals along the treatment path of wastewater in subsurface-flow constructed wetlands. Water Sci Technol 48:307–313
Liu SX, Athar M, Lippai I, Charles W et al (2000) Induction of oxyradicals by arsenic: implication for mechanism of genotoxicity. PNAS 98:1643–1648
Liu S, Zhang F, Chen J, Sun G (2011) Arsenic removal from contaminated soil via biovolatilization by genetically engineered bacteria under laboratory conditions. J Environ Sci 23:1544–1550
Maestri E, Marmiroli M (2011) Genetic and molecular aspects of metal tolerance and hyperaccumulation. Metal Tox Plants:41–61
Maitani T, Kubota H, Sato K, Yamada T (1996) The composition of metals bound to class III metallothionein (phytochelatin and its desglycyl peptide) induced by various metals in root cultures of Rubia tinctorum. Plant Physiol 110:1145–1150
Malik A (2004) Metal bioremediation through growing cells. Environ Int 30:261–278
Mandal BK, Suzuki KT (2002) Arsenic round the world: a review. Talanta 58:201–235
Mazumder DN, De BK, Santra A, Gupta JD et al (1999) Chronic arsenic toxicity, epidemiology, natural history and treatment. In: Chappell WR, Abernathy CO, Calderon RL (eds) Arsenic exposure and health effects. Elsevier Science, New York, pp 335–347
Mejare M, Bulow L (2001) Metal-binding proteins and peptides in bioremediation and phytoremediation of heavy metals. Trends Biotechnol 19:67–73
Mello-Farias PC, Chaves ALS (2008) Biochemical and molecular aspects of toxic metals phytoremediation using transgenic plants. In: Tiznado-Hernandez ME, Troncoso-Rojas R, RiveraDomĂnguez MA (eds) Transgenic approach in plant biochemistry and physiology. Research Signpost, Kerala, pp 253–266
Mergeay M, Monchy S, Vallaeys T, Auquier V et al (2003) Ralstonia metallidurans, a bacterium specifically adapted to toxic metals: towards a catalogue of metal-responsive genes. FEMS Microbiol Rev 27:385–410
Mohanpuria P, Rana NK, Yadav SK (2007) Cadmium induced oxidative stress influence on glutathione metabolic genes of Camellia sinensis. Environ Toxicol 22:368–374
Munawar S, Susann V, Kamrun Z, Christine SA (2012) New clusters of arsenite oxidase and unusual bacterial groups in enrichments from arsenic-contaminated soil. Arch Microbiol 194:623–635
Naik MM, Dubey SK (2013) Lead resistant bacteria: lead resistance mechanisms, their applications in lead bioremediation and biomonitoring. Ecotoxicol Environ Saf 98:1–7
National Academy of Science (1977) Arsenic. Author, Washington, DC
Nies DH (1999) Microbial heavy-metal resistance. Appl Microbiol Biotechnol 51:730–750
Ow DW (1996) Heavy metal tolerance genes-prospective tools for bioremediation. Resour Conserv Recycl 18:135–149
Pacyna EG, Pacyna JM, Steenhuisen F (2006) Global anthropogenic mercury emission inventory for 2000. Atmos Environ 40:4048–4063
Patel J, Zhang Q, McKay LMR, Vincent R (2010) Genetic engineering of Caulobacter crescentus for removal of cadmium from water. Appl Biochem Biotechnol 160:232–243
Qin J, Rosen BP, Zhang Y, Wang GJ et al (2006) Arsenic detoxification and evolution of trimethylarsine gas by a microbial arsenite S-adenosylmethionine methyltransferase. Proc Natl Acad Sci U S A 103:2075–2080
Raskin I, Ensley BD (2002) Phytoremediation of toxic metals using plants to clean the environment. John Wiley & Sons, New York, p 304
Saberi M, Tavali A, Jafari M, Heidari M (2010) The effect of different levels of heavy metals on seed germination and seedling growth of Atriplex lentiformis. J Range Manag 4:112–120
Salonen JT, Seppanen K, Nyyssonen K, Korpela H et al (1995) Intake of mercury from fish, lipid peroxidation, and the risk of myocardial infarction and coronary, cardiovascular, and any death in eastern Finnish men. Circulation 91:645–655
Santra A, Maiti A, Das S, Lahiri S et al (2000) Hepatic damage caused by chronic arsenic toxicity in experimental animals. Clin Toxicol 38:395–405
Satarug S, Moore MR (2004) Adverse health effects of chronic exposure to low-level cadmium in foodstuffs and cigarette smoke. Environ. Health Perspect 112:1099–1103
Sauge-Merle S, Cuine S, Carrier P, Lecomte-Pradines C et al (2003) Enhanced toxic metal accumulation in engineered bacterial cells expressing Arabidopsis thaliana phytochelatin synthase. Appl Environ Microbiol 69:490–494
Selvi MS, Sasikumar S, Gomathi S, Rajkumar P et al (2014) Isolation and characterization of arsenic resistant bacteria from agricultural soil, and their potential for arsenic bioremediation. Int J Agric Policy Res 2:393–405
Sharma S (2012) Bioremediation: features, strategies and applications. Asian J Pharm Life Sci 2:202–213
Singh BK (2011) Emerging and genomic approaches in bioremediation. In proceedings of the 4th international contaminated site remediation conference, Adelaide, Australia, 11–15
Sinha A, Khare SK (2012) Mercury bioremediation by mercury accumulating Enterobacter sp. cells and its alginate immobilized application. Biodegradation 71:1–10
Smedley PL, Nicolli HB, Macdonald DMJ, Barros AJ et al (2002) Hydro-geochemistry of arsenic and other inorganic constituents in ground-waters from La Pampa. Argentina Appl Geochem 17:259–284
Sriprang R, Hayashi M, Ono H, Takagi M et al (2003) Enhanced accumulation of Cd2+ by a Mesorhizobium sp. transformed with a gene from Arabidopsis thaliana coding for phytochelatin synthase. Appl Environ Microbiol 69:179–796
Streets DG, Zang Q, Wu Y (2009) Projections of global mercury emissions in 2050. Environ Sci Technol 43:2983–2988
Tchounwou PB, Patlolla AK, Centeno JA (2003) Carcinogenic and systemic health effects associated with arsenic exposure—a critical review. Toxicol Pathol 31:575–588
Titus JA, Pfister RM (1984) Bacteria and cadmium interactions in natural and laboratory model aquatic systems. Arch Environ Contam Toxicol 13:271–277
Tong S, Schirnding V, Prapamontol YE (2000) Environmental lead exposure: a public health problem of global dimensions. Bullet. World Health Org 78:1068–1077
Turpeinen R, Kallio MP, Kairesalo T (2002) Role of microbes in controlling the speciation of arsenic and production of arsines in contaminated soils. Sci. Total Environ 285:133–145
UNEP (2003) Global mercury assessment. United Nations Environment Programme, Geneva
Utsunamyia T (1980) Japanese patent application no. pp- 55-72959
Vadkertiova R, Slavikova E (2006) Metal tolerance of yeasts isolated from water. J Basic Microbiol 46:145–152
Valls M, Lorenzo VD (2002) Exploiting the genetic and biochemical capacities of bacteria for the remediation of heavy metal pollution. FEMS Microbiol Rev 26:327–338
Valls M, Atrian S, Lorenzo V, Fernadéz LA (2000) Engineering a mouse metallothionein on the surface of Ralstonia eutropha CH34 for immobilization of heavy metals in soil. Nat Biotechnol 18:661–665
Wagner-Dobler I, Lunsdorf H, Lubbenhausen T, Von C et al (2000) Structure and species composition of mercury reducing biofilms. Appl Environ Microbiol 66:4559–4563
Wang Y, Moore M, Levinson HS, Silver S (1989) Nucleotide sequence of a chromosomal mercury resistance determinant from a Bacillus sp. With broad-spectrum mercury resistance. J Bacteriol 171:83–92
Watt GCM, Britton A, Gilmour HG, Moore MR et al (2000) Public health implications of new guidelines for lead in drinking water: a case study in an area with historically high water leads levels. Food Chem Toxicol 38:73–79
Weiss B, Clarkson TW, Simon W (2002) Silent latency periods in methylmercury poisoning and in neurodegenerative disease. Environ Health Perspect 110:851–856
WHO (1992) Cadmium—environmental aspects (environmental health criteria 135). World Health Organization, Geneva
WHO (2008) Guideline for drinking water quality recommendations, vol 1. World Health Organization, Geneva
Xu C, Rosen BP (1997) Dimerization is essential for DNA binding and repression by the ArsR metalloregulatory protein of Escherichia coli. J Biol Chem 272:15734–15738
Yang CXL (2010) Construction of a genetically engineered microorganism with high tolerance to arsenite and strong arsenite oxidative ability. J Environ Sci Health Tox Hazd Subst Environ Eng 45:732–737
Young RA (2005) Toxicity Profiles: Toxicity Summary for Cadmium, Risk Assessment Information System, RAIS, University of Tennessee (http://www.rais.ornl.gov/tox/profiles/cadmium.shtml)
Yuan CG, Lu XF, Qin J, Rosen BP et al (2008) Volatile arsenic species released from Escherichia coli expressing the AsIII S-adenosylmethionine methyltransferase gene. Environ Sci Technol 42:3201–3206
Zaki S, Farag S (2010) Isolation and molecular characterization of some copper biosorped strains. Int J Environ Sci Technol 7:553–560
Zeng X, Tang J, Jiang P, Liu H et al (2010) Isolation, characterization and extraction of mer gene of Hg2+ resisting strain D2. Trans Nonferrous Met Soc 20(507):512
Zhou ZS, Huang SQ, Guo K, Mehta SK (2007) Metabolic adaptations to mercury-induced oxidative stress in roots of Medicago sativa L. J Inorg Biochem 101:1–9
Zhu YL, Smitis EAHP, Jouanin L, Terry N (1999) Over-expression of glutathione synthetase in Indian mustard enhances cadmium accumulation and tolerance. Plant Physiol 119:73–79
Zynda T (2001) Fact, Michigan State University TAB Program
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Gupta, S., Singh, D. (2017). Role of Genetically Modified Microorganisms in Heavy Metal Bioremediation. In: Kumar, R., Sharma, A., Ahluwalia, S. (eds) Advances in Environmental Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-10-4041-2_12
Download citation
DOI: https://doi.org/10.1007/978-981-10-4041-2_12
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-10-4040-5
Online ISBN: 978-981-10-4041-2
eBook Packages: Earth and Environmental ScienceEarth and Environmental Science (R0)