Abstract
The study highlights the potential of Col3.6 transgenic fluorescent reporter mouse models to understand BMP mediated localized bone formation, using enzymatically crosslinkable glycol chitosan hydrogel as a delivery vehicle. Using the injectable gel, rhBMP-2 (0.5 µg/defect) was able to fully regenerate the critical sized calvarial defect in vivo at 8 weeks. At a cellular level, the tissue showed the presence of mature osteoblasts (bright green) with active mineralization (AC) and osteogenic activity (ALP) as well as presence of osteoclasts (TRAP) carrying out active remodeling, indicating the quality and maturation of the mineralized, regenerated tissue.
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Gohil, S.V., Nair, L.S. (2017). Evaluation of BMP-2 Mediated Bone Formation Using Enzymatically Crosslinkable Injectable Hydrogels: An In Vivo Study Using Transgenic Fluorescent Reporter Mouse Model. In: Shorey, R., Ghosh, P. (eds) Healthcare Engineering. Springer, Singapore. https://doi.org/10.1007/978-981-10-3111-3_8
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DOI: https://doi.org/10.1007/978-981-10-3111-3_8
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