Abstract
The procedure of enzyme immobilization in biosensorics has a purpose to induce maximal activity of biological element with sufficient stability and reusability of the bioelectrodes. The entrapment of the biomaterial with a porous solid matrix looks most promising in this respect due to minimal alteration of enzyme structure and behavior. On the other hand, the method needs pore-size control to reduce the diffusional limitation of the substrate to the enzyme. We propose the use of photocross-linked polymers consisted of epoxidized linseed oil (ELO) as a matrix for immobilization of fungal laccase. The network properties of the polymer matrixes, holding the biosensing element, were studied by positron annihilation lifetime spectroscopy measurements. The correlation between the operational parameters of the constructed biosensor and the microscopical free volume of the holding photocross-linking matrixes was established.
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Acknowledgments
This work was supported in part by the Ministry of Education and Science of Ukraine (projects Nos. 0117U007142, 0118U000297, and 0119U100671), National Academy of Sciences of Ukraine in the frame of the Scientific-Technical Program “Smart sensor devices of a new generation based on modern materials and technologies” (project No. 13), Slovak Grant Agency VEGA (project No. 2/0157/17) and Slovak Research and Development Agency (project No. APVV-16-0369).
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Kavetskyy, T. et al. (2020). Novel Photocross-Linked Polymers for Construction of Laccase-Based Amperometric Biosensors. In: Petkov, P., Achour, M., Popov, C. (eds) Nanoscience and Nanotechnology in Security and Protection against CBRN Threats. NATO Science for Peace and Security Series B: Physics and Biophysics. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-2018-0_24
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DOI: https://doi.org/10.1007/978-94-024-2018-0_24
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