Abstract
A gentle osmotic shock of intact and purified chloroplasts allows the preparation of envelope membranes in a reasonably pure state. Unfortunately, irreversible changes take place in the membranes (fusions?) during the osmotic shock thus making the separation of the outer membrane from the inner impossible (Douce, Joyard, 1979). Such a separation can only be achieved by strikingly different procedures. Cline et al. (1981) have described a procedure which includes freeze-thaw lysis of intact pea chloroplasts. We have developed a different method to provide the separation of two membrane fractions deriving from the chloroplast envelope membranes. Since we have characterized two polypeptides as outer envelope polypeptides (Joyard et al., 1983), we were able to characterize the membrane fractions obtained (Block et al., in press).
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References
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© 1984 Springer Science+Business Media Dordrecht
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Block, M.A., Dorne, AJ., Joyard, J., Douce, R. (1984). Preparation and Characterization of Membrane Fractions Enriched in Outer and Inner Envelope Membranes from Spinach Chloroplasts. In: Sybesma, C. (eds) Advances in Photosynthesis Research. Advances in Agricultural Biotechnology, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-4973-2_5
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DOI: https://doi.org/10.1007/978-94-017-4973-2_5
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-247-2944-9
Online ISBN: 978-94-017-4973-2
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