Abstract
Ethylene regulates the processes of petal senescence in both carnation and petunia. To investigate the relationship between transcriptional regulation of ethylene production and petal senescence, we have cloned genes involved in ethylene biosynthesis from the petals of these species. Using the polymerase chain reaction we have isolated a cDNA clone encoding l-aminocyclopropane-1-carboxylic acid (ACC) synthase from petunia. Heterologous screening of a senescing petal cDNA library provided an ACC synthase encoding clone from carnation. Increased levels of the mRNAs hybridizing to these cDNA clones is observed in climacteric petals and can be correlated with the onset of senescence. A cDNA clone encoding ACC oxidase was isolated by differential screening of a senescing carnation petal library. Antisense expression of ACC oxidase in transgenic carnation plants inhibits petal inrolling, increasing the post harvest life of the flowers.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Literature Cited
Borochov, A., Mayak, S.and Brown, R., (1982) ‘The involvement of water stress and ethylene in senescence of cut carnation flowers’, J. Exp. Bot. 33: 1202–1209
Cook, E.L. and Van Staden, (1988) ‘The carnation as a model for hormonal studies in flower senescence’, Plant Physiol. Biochem. 26: 793–807
Frohman, M.A., Dush, M.K. and Martin G.R., (1988) ‘Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer’, Proc. Nat. Acad. Sci.,USA 85: 8998–9001
Hamilton, A.J., Lycett, G.W. and Grierson, D., (1990) ‘Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants’, Nature 346: 284–287
Lu, C-Y., Nugent, G., Wardley-Richardson, T., Chandler, S.F., Young, R., and Dalling, M.J. (1991) ’Agrobacterium-mediated transformation of carnation (Dianthus caryophyllus L.)’, Biotechnology 9: 846–868
Geller, P.W., Min-Wong, L., Taylor, L.P., Pike, D.A. and Theologis, A. (1991) ‘Reversible inhibition of tomato fruit senescence by antisense RNA’, Science 254: 437–439
Sato, T. and Theologis, A., (1989) ‘Cloning the mRNA encoding 1-aminocyclopropane-lcarboxylate synthase, the key enzyme for ethylene biosynthesis in plants’, Proc. Natl. Acad. Sci., USA, 86: 6621–6625
Van Der Straeten, D., Van Wiemeersch, L., Goodman, H.M. and Van Montagu, M., (1990) ‘Cloning and sequence of two different cDNAs encoding 1-aminocyclopropane-1carboxylate synthase in tomato’, Proc. Nat. Acad. Sci., USA, 87: 4859–4863
Veen H. (1986) ‘A theoretical model for anti-ethylene effects of silver thiosulphate and 2,5norbornadiene’, Acta Horticulturae 181: 129–134
Wang, H., and Woodson, W.R., (1991) ‘A flower senescence-related mRNA from carnation shares sequence similarity with fruit ripening-related mRNAs involved in ethylene biosynthesis’, Plant Physiology 96: 1000–1001
Woodson, W.R., (1987) ‘Changes in protein and mRNA populations during the senescence of carnation petals’, Physiologia Plantarum 71: 495–502
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Michael, M.Z. et al. (1993). Cloning of Ethylene Biosynthetic Genes Involved in Petal Senescence of Carnation and Petunia, and Their Antisense Expression in Transgenic Plants. In: Pech, J.C., Latché, A., Balagué, C. (eds) Cellular and Molecular Aspects of the Plant Hormone Ethylene. Current Plant Science and Biotechnology in Agriculture, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1003-9_68
Download citation
DOI: https://doi.org/10.1007/978-94-017-1003-9_68
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-4249-1
Online ISBN: 978-94-017-1003-9
eBook Packages: Springer Book Archive