Abstract
Nicotiana tabacum cv. Wisconsin W38 expressing the tetracycline repressor protein (TetR) was transformed with the harpin structural gene from Pseudomonas syringae pv. phaseolicola (hrpZ Psp ) using a Ti vector that provided tetracycline-dependent transgene induction. The constructs were designed to express either the canonical form of harpin (HrpZPsp) or a N-terminally extended version of the protein carrying the signal peptide portion of the tobacco pathogenesis related protein PR1a. Following induction with tetracycline, leaves of transgenic lines accumulated HrpZpsP in quantities comparable to those seen during bacterially-induced HR but showed no necrosis. The amount of harpin present per unit leaf area at 8 hr after induction with tetracycline was weil above the amount produced in the leaf by bacterial inocula eliciting confluent HR, but much lower than the quantity of purified harpin necessary for necrosis induction. Very high concentartions of the canonical version of harpin were also obtained in Nicotiana benthamiana leaves after infection with a potato virus X (PVX)-hrpZpsp construct. However, no necrotic symptoms were observed, eventhough protein extracts from these plants elicited typical HR when injected in N. tabaccum leaves. Our results suggest that harpin, or another eficitor, produced in plants after infection with the PVX vector expressing hrpZPsp, is HR active in the standard leaf injection assay. Saccharomyces cerevisiae cells transformed with galactose-inducible promoter plasmids carrying a hrpZPsp in-frame fusion to the leader peptide of the á mating factor produced readily detectable amounts of OF harpin but showed no obvious effects on growth.
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© 1997 Springer Science+Business Media Dordrecht
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Tabakaki, N., Hatziloukas, E., Grimm, C., Panopoulos, N.J. (1997). Expression of the Pseudomonas syringae pv. phaseolicola HrpZ Gene in Transgenic Tobacco and Saccharomyces cerevisiae . In: Rudolph, K., Burr, T.J., Mansfield, J.W., Stead, D., Vivian, A., von Kietzell, J. (eds) Pseudomonas Syringae Pathovars and Related Pathogens. Developments in Plant Pathology, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5472-7_71
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DOI: https://doi.org/10.1007/978-94-011-5472-7_71
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