Abstract
During the past decade there has been remarkable progress in the understanding and practical use of chlorophyll fluorescence in plant science (1,2,3). This progress has resulted from fruitful interactions between three different research disciplines: basic research (dealing with the dynamics of excitation transfer, photochemical charge separation and electron transport), applied research (making use of fluorescence as an noninvasive tool) and the development of new instruments and methodology, to measure fluorescence and to extract the essential information from it. In particular, with the introduction of pulse-amplitude-modulated (PAM) fluorometers and the saturation pulse method of quenching analysis (4), chlorophyll fluorescence has gained widespread applications. It provides manifold information at various levels of the complex process of photosynthesis, starting from light absorption, energy transfer and primary energy conversion, and ending with the export of assimilates from the chloroplast. Due to its large signal amplitude, chlorophyll fluorescence traditionally has been a pioneering tool in photoynthesis research. Making use of recent progress in optoelectronics and microprocessor/computer technology, a new generation of chlorophyll fluorometers with extreme sensitivity and selectivity has been developed, the essential features of which will be outlined in the present communication.
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References
van Kooten, O. and Snel, J. (1990) Photosynthesis Res. 25, 147–150
Schreiber, U., Bilger, W. and Neubauer, C. (1994) in Ecophysiology of Photosynthesis (Schulze, E.D. and Caldwell, M.M, eds.) pp. 49–70, Springer, Berlin
Dau, H. (1994) Photochem. Photobiol. 60, 1–23
Schreiber, U., Schliwa, U. and Bilger, W. (1986) Photosynth. Res. 10, 51–62
Lorenzen, C.J. (1966) Deep-Sea Research 13, 223–227
Falkowski, P.G. and Kolber, Z. (1995) Aust. J. Plant Physiol. 22, 341–355
Kolber, Z. and Falkowski, P.G. (1993) Limnology and Oceanography 38, 1646–1665
Schreiber, U., Schliwa, U. and Neubauer, C. (1993) Photosynth. Res. 36, 65–72
Kolbowski, J. and Schreiber, U. (1995) in Photosynthesis: from Light to Biosphere (Mathis, P. ed.) pp. 825–828, Kluwer Academic Publishers, Dordrecht, The Netherlands
Genty, B., Briantais, J.-M. and Baker, N.R. (1989) Biochim. Biophys. Acta 990, 87–92
Mi, H., Endo, T., Schreiber, U. Ogawa, T. and Asada, K. (1992) Plant Cell Physiol. 33, 1233–1237
Allen, J.F. (1991) Biochim. Biophys. Acta 1098, 275–335
Schreiber, U., Hormann, H., Asada, K. and Neubauer, C. (1995) in Photosynthesis: from Light to Biosphere (Mathis, P. ed.) pp. 813–818, Kluwer Academic Publishers, Dordrecht, The Netherlands
Schreiber, U., Kühl, M., Klimant, I. and Reising, H. (1996) Photosylith. Res. 47, 103–109
Kühl, M. and Jorgensen, BB. (1992) Limnol. Oceanogr. 37, 1813–1823
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© 1998 Springer Science+Business Media Dordrecht
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Schreiber, U. (1998). Chlorophyll fluorescence: New Instruments for Special Applications. In: Garab, G. (eds) Photosynthesis: Mechanisms and Effects. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3953-3_984
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DOI: https://doi.org/10.1007/978-94-011-3953-3_984
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