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Part of the book series: Molecular and Cell Biology of Human Diseases Series ((Mol. Cell Biol. Hu. Dis.,volume 20))

Abstract

Nucleic acid-mediated immunization refers to the induction of an immune response to a protein expressed in vivo subsequent to the introduction of its encoding polynucleotide (DNA or RNA). If proven to be safe, nucleic acid vaccines may one day be an attractive alternative to classical vaccines, which contain the antigen itself.

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References

  • Acsadi, G., Dickson, G., Love, D.R. et al. (1991) Human dystrophin expression in mdx mice after intramuscular injection of DNA constructs. Nature, 352, 815–18.

    Article  CAS  PubMed  Google Scholar 

  • Cheng, L., Ziegelhoffer, P.R. and Yang, N.S. (1993) In vivo promoter activity and transgene expression in mammalian somatic tissues evaluated by using particle bombardment. Proc. Natl Acad. Sci. USA, 90, 4455–9.

    Article  CAS  PubMed  Google Scholar 

  • Conry, R.M., LoBuglio, A.F., Kantor, J. et al. (1994) Immune response to a carcinoembryonic antigen polynucleotide vaccine. Cancer Res., 54, 1164–8.

    CAS  PubMed  Google Scholar 

  • Cox, G.J., Zamb, T.J. and Babiuk, L.A. (1993) Bovine herpesvirus 1: immune responses in mice and cattle injected with plasmid DNA. J. Virol., 67, 5664–7.

    CAS  PubMed Central  PubMed  Google Scholar 

  • d’Albis, A., Couteaux, R., Janmot, C. and Mira, J.-C. (1989) Myosin isoform transitions in regeneration of fast and slow muscles during postnatal development of the rat. Dev. Biol., 135, 320–5.

    Article  PubMed  Google Scholar 

  • Danko, I., Fritz, J.D., Jiao, S. et al. (1994) Pharmacological enhancement of in vivo foreign gene expression in muscle. Gene Ther., 1, 114–21.

    CAS  PubMed  Google Scholar 

  • Davis, H.L. and Jasmin, B.J. (1993) Direct gene transfer into mouse diaphragm. FEBS Lett., 333, 146–50.

    Article  CAS  PubMed  Google Scholar 

  • Davis, H.L., Demeneix, B.A., Quantin, B. et al. (1993a) Plasmid DNA is superior to viral vectors for direct gene transfer in adult mouse skeletal muscle. Human Gene Ther., 4, 733–40.

    Article  CAS  Google Scholar 

  • Davis, H.L., Michel, M.-L. and Whalen, R.G. (1993b) DNA based immunization for hepatitis B induces continuous secretion of antigen and high levels of circulating antibody. Human Mol. Genet., 2, 1847–51.

    Article  CAS  Google Scholar 

  • Davis, H.L., Whalen, R.G. and Demeneix, B.A. (1993c) Direct gene transfer into skeletal muscle in vivo: factors affecting efficiency of transfer and stability of expression. Human Gene Ther., 4, 151–9.

    Article  Google Scholar 

  • Davis, H.L., Michel, M.-L., Mancini, M. et al. (1994) Direct gene transfer in muscle with plasmid DNA for the purpose of nucleic acid immunization. Vaccine, 12, 1503–9.

    Article  CAS  PubMed  Google Scholar 

  • Eisenbraun, M.D., Fuller, D.H. and Haynes, J.R. (1993) Examination of parameters affecting the elicitation of humoral immune responses by particle bombardment-mediated genetic immunization. DNA Cell Biol., 12, 791–7.

    Article  CAS  PubMed  Google Scholar 

  • Furth, P.A., Shamay, A., Wall, R.J. and Hennighausen, L. (1992) Gene transfer into somatic tissues by jet injection. Anal. Biochem., 205, 365–8.

    Article  CAS  PubMed  Google Scholar 

  • Fynan, E.F., Robinson, H.L. and Webster, R.G. (1993a) Use of DNA encoding influenza hemagglutinin as an avian influenza vaccine. DNA Cell Biol., 12, 785–9.

    Article  CAS  PubMed  Google Scholar 

  • Fynan, E.F., Webster, R.G., Fuller, D.H. et al. (1993b) DNA vaccines: protective immunizations parental, mucosal, and gene-gun inoculations. Proc. Natl Acad. Sci. USA, 90, 11478–82.

    Article  CAS  PubMed  Google Scholar 

  • Geissler, E.K., Wang, J., Fechner, J.H. et al. (1994) Immunity to MHC class I antigen after direct DNA transfer into skeletal muscle. J. Immunol., 152, 413–21.

    CAS  PubMed  Google Scholar 

  • Jiao, S., Williams, P., Berg, R.K. et al. (1992) Direct gene transfer into nonhuman primate myofibers in vivo. Human Gene Ther., 3, 21–33.

    Article  CAS  Google Scholar 

  • Kuby, J. (1994) Immunology W.H. Freeman & Co., New York, 1994.

    Google Scholar 

  • Martinon, F., Krishnan, S., Lenzen, G. et al. (1993) Induction of virus-specific cytotoxic T lymphocytes in vivo by liposome-entrapped mRNA. Eur. J. Immunol., 23, 1719–22.

    Article  CAS  PubMed  Google Scholar 

  • Michel, M.-L., Davis, H.L., Mancini, M. et al. (1995) DNA-based immunization to hepatitis B surface antigen in mice: humoral response mimics that with natural hepatitis B viral infection in humans. Proc. Natl Acad. Sci. USA, in press.

    Google Scholar 

  • Montgomery, D.L., Shiver, J.W., Leander, K.R. et al. (1993) Heterologous and homologous protection against influenza A by DNA vaccination: optimization of DNA vectors. DNA Cell Biol., 12, 777–83.

    Article  CAS  PubMed  Google Scholar 

  • Nabel, E.G., Plautz, G. and Nabel, G.J. (1992) Transduction of a foreign histocompatibility gene into the arterial wall induces vasculitis. Med. Sci. 89, 5157–61.

    CAS  Google Scholar 

  • Pecorino, L.T. and Lo, D.C. (1992) Gene transfections: having a blast with gene transfer. Curr. Biol., 2, 30–2.

    Article  CAS  PubMed  Google Scholar 

  • Robinson, H.L., Hunt, L.A. and Webster, R.G. (1993) Protection against a lethal influenza virus challenge by immunization with a haemagglutinin-expressing plasmid DNA. Vaccine, 11, 957–60.

    Article  CAS  PubMed  Google Scholar 

  • Sedegah, M., Hedstrom, R., Hobart, P. and Hoffman, S.L. (1994) Protection against malaria by immunization with plasmid DNA encoding circumsporozoite protein. Proc. Natl Acad. Sci. USA, 91, 9866–70.

    Article  CAS  PubMed  Google Scholar 

  • Tang, D., DeVit, M. and Johnston, S.A. (1992) Genet immunization is a simple method for eliciting an immune response. Nature, 356, 152–4.

    Article  CAS  PubMed  Google Scholar 

  • Ulmer, J.B., Donnelly, J.J., Parker, S.E. et al. (1993) Heterologous protection against influenza by injection of DNA encoding a viral protein. Science, 259, 1745–9.

    Article  CAS  PubMed  Google Scholar 

  • Vitadello, M., Schiaffino, M.V., Picard, A. et al. (1994) Gene transfer in regenerating muscle. Human Gene Ther., 5, 11–17.

    Article  CAS  Google Scholar 

  • Wang, B., Boyer, J., Srikantan, V. et al. (1993a) DNA inoculation induces neutralizing immune responses against human immunodeficiency virus type 1 in mice and nonhuman primates. DNA Cell. Biol. 12, 799–805.

    Article  CAS  PubMed  Google Scholar 

  • Wang, B., Ugen, K.E., Srikantin, V. et al. (1993b) Gene inoculation generates immune responses against human immunodeficiency virus type I. Proc. Natl Acad. Sci. USA, 90, 4156–60.

    Article  CAS  PubMed  Google Scholar 

  • Whalen, R.G. (1994) Promoters, enhancers and inducible elements for gene therapy, in Gene Therapeutics, (ed. J.A. Wolff), Birkhäuser, Boston, pp. 60–79.

    Chapter  Google Scholar 

  • Whalen, R.G., Harris, J.B., Butler-Browne, G.S. and Sesodia, S. (1990) Expression of myosin isoforms during notexin-induced regeneration of rat soleus muscles. Dev. Biol., 141, 24–40.

    Article  CAS  PubMed  Google Scholar 

  • Williams, W.V., Boyer, J.D., Merva, M. et al. (1993) Genetic infection induces protective in vivo immune responses. DNA Cell Biol., 12, 675–83.

    Article  CAS  PubMed  Google Scholar 

  • Wolff, J.A., Malone, R.W., Williams, P. et al. (1990) Direct gene transfer into mouse muscle in vivo. Science, 247, 1465–8.

    CAS  Google Scholar 

  • Wolff, J.A., Williams, P., Acsadi, G. et al. (1991) Conditions affecting direct gene transfer into rodent muscle in vivo. Bio Techniques, 11, 474–85.

    CAS  Google Scholar 

  • World Health Organization Programme for Vaccine Development (1990) Potential use of live viral and bacterial vectors for vaccines. Vaccine, 8, 425–37.

    Article  Google Scholar 

  • Xiang, Z.Q., Spitalnik, S., Tran, M. et al. (1994) Vaccination with a plasmid vector carrying the rabies virus glycoprotein gene induces protective immunity against rabies virus. Virology, 199, 132–40.

    Article  CAS  PubMed  Google Scholar 

  • Yankauckas, M.A., Morrow, J.E., Parker, S.E. et al. (1993) Long-term anti-nucleoprotein cellular and humoral immunity is induced by intramuscular injection of plasmid DNA containing NP gene. DNA Cell Biol., 12, 771–6.

    Article  CAS  PubMed  Google Scholar 

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© 1995 Springer Science+Business Media Dordrecht

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Davis, H.L., Whalen, R.G. (1995). DNA-based immunization. In: Dickson, G. (eds) Molecular and Cell Biology of Human Gene Therapeutics. Molecular and Cell Biology of Human Diseases Series, vol 20. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0547-7_18

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  • DOI: https://doi.org/10.1007/978-94-011-0547-7_18

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4242-0

  • Online ISBN: 978-94-011-0547-7

  • eBook Packages: Springer Book Archive

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