Abstract
Primary cultures of beating rat heart muscle cells were shown to be a good model system for studies of iron uptake and intracellular iron metabolism. Iron uptake from several iron sources was rapid and ferritin was visualized (by electron microscopy) within 48–96 hours after addition of iron. Iron was distributed in various cellular fractions; an iron-containing cytosol component(s) (non-ferritin) that may represent a “pool” of iron was evident. The cultured rat myocytes were used to screen chelators that might become useful in therapy of human iron overload. One chelator, agrobactin, was significantly more effective than the drug deferoxamine in reduction of iron uptake from transferrin-iron by the cells; however, enterobactin increased iron uptake from this source. Deferoxamine inhibited incorporation of iron into ferritin and increased the amount of iron in the non-ferritin cytosol fraction. Therefore, some of these chelators may be useful probes in studies of intracellular iron metabolism.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Jacobs, A., and Worwood, M. (eds.): 1974, Iron in Biochemistry and Medicine, Academic Press, New York.
Muller-Eberhard, U., Mieseher, P.A., and Jaffe, E.R. (eds.): 1977, Iron Excess: Aberrations of Iron and Porphyrin Metabolism, Grune and Stratton, New York.
Bezkorovainy, A.: 1980, Biochemistry of Nonheme Iron, Plenum Press, New York.
Jacobs, A.: 1977, in Iron Metabolism, CIBA Foundation Symposium, Elsevier, Amsterdam, pp. 91–106.
Trump, B. F., and Beresky, I.K.: 1977, in Proteins of Iron Metabolism, E. B. Brown, P. Aisen, J. Fielding, and R. R. Crichton (eds.), Grune and Stratton, New York, pp. 359–364.
Munro, H. N., and Linder, M. C.: 1978, Physiol. Rev. 58, pp. 317–396.
Bailey-Wood, R., White, G. P., and Jacobs, A.: 1975, Br. J. Exp. Path. 56, pp. 358–362.
Hoy, T. G. and Jacobs, A.: 1981, Biochem. J. 193, pp. 87–92.
Sciortino, C.V., Byers, B.R., and Cox, P.: 1980, J. Lab. Clin. Med. 96, pp. 1081–1085.
Cox, P. G., Harvey, N. E., Sciortino, C., and Byers, B. R,: 1981, Am. J. Pathol. 102, pp. 151–159.
Sciortino, C.V.: 1980, Ph.D. Dissertation, University of Mississippi Medical Center.
Sciortino, C.V., Cox, P.G., and Byers, B.R., manuscript submitted.
Harris, W.R., Raymond, K.N., and Weitl, F.L.: 1981, J. Am. Chem. Soc. 103, pp. 2667–2675.
Venuti, M.C., Rastetter, W.H. and Neilands, J.B.: 1979, J. Med. Chem. 22, pp. 123–124.
Lodge, J.S., Gaines, C.G., Arceneaux, J.E.L., and Byers, B.R.: 1980, Biochem. Biophys. Res. Commun. 97, pp. 1291–1295.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1982 D. Reidel Publishing Company
About this paper
Cite this paper
Byers, B.R., Sciortino, C.V., Cox, P., Robinson, P. (1982). Iron Uptake and Intracellular Iron Distribution in Cultured Rat Heart Cells: Effects of Iron Chelators. In: Dunford, H.B., Dolphin, D., Raymond, K.N., Sieker, L. (eds) The Biological Chemistry of Iron. NATO Advanced Study Institutes Series, vol 89. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-7882-9_8
Download citation
DOI: https://doi.org/10.1007/978-94-009-7882-9_8
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-009-7884-3
Online ISBN: 978-94-009-7882-9
eBook Packages: Springer Book Archive