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Frankia Symbioses pp 61–78Cite as

Growth kinetics and nitrogenase induction in Frankia sp. HFPArI 3 grown in batch culture

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Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 12))

Summary

Kinetics of growth and nitrogenase induction in Frankia sp. ArI3 were studied in batch culture. Growth on defined medium with NH +4 as the N source displayed typical batch culture kinetics; however, a short stationary phase was followed by autolysis. Removal of NH +4 arrested growth and initiated vesicle differentiation. Vesicle numbers increased linearly and were paralleled by a rise in nitrogenase (acetylene reduction) activity. Nitrogenase activity (10 mM C2H4 · mg protein−1 · min−1) was sufficient to support growth on N2 and protein levels rose in parallel with nitrogenase induction. Optimal conditions for vesicle and nitrogenase induction were investigated. Maximum rates of acetylene reduction were obtained with 5 to 10 mM K2 HPO4/KH2PO4, 0.1 mM CaCl2 and MgSO4. The optimum pH for acetylene reduction and respiration was around 6.7. The amount (5 to 10µg protein/ml) and stage (exponential) of growth of the ammonium-grown inoculum strongly influenced the subsequent development of nitrogenase activity. Propionate was the most effective carbon source tested for nitrogenase induction. Respiration in propionate-grown cells was stimulated by CO2 and biotin, suggesting that propionate is metabolized via the propionyl CoA pathway.

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Author information

Authors and Affiliations

  1. Cabot Foundation, Harvard University, Petersham, MA, 01366, USA

    Marcia A. Murry, Mark S. Fontaine & John G. Torrey

Authors
  1. Marcia A. Murry
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  2. Mark S. Fontaine
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  3. John G. Torrey
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Editor information

Editors and Affiliations

  1. Department of Microbiology, Agricultural University of Wageningen, Wageningen, The Netherlands

    A. D. L. Akkermans

  2. C. F. Kettering Research Laboratory, Yellow Springs, Ohio, USA

    D. Baker

  3. Department of Plant Physiology, University of Umeå, Umeå, Sweden

    K. Huss-Danell

  4. Department of Botany and Plant Pathology, University of Maine, Orono, Maine, USA

    J. D. Tjepkema

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© 1984 Martinus Nijhoff/Dr W. Junk Publishers, The Hague

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Murry, M.A., Fontaine, M.S., Torrey, J.G. (1984). Growth kinetics and nitrogenase induction in Frankia sp. HFPArI 3 grown in batch culture. In: Akkermans, A.D.L., Baker, D., Huss-Danell, K., Tjepkema, J.D. (eds) Frankia Symbioses. Developments in Plant and Soil Sciences, vol 12. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-6158-6_7

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  • DOI: https://doi.org/10.1007/978-94-009-6158-6_7

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-009-6160-9

  • Online ISBN: 978-94-009-6158-6

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