Skip to main content

Vitrification: morphological and physiological disorders of in vitro plants

  • Chapter
Micropropagation

Abstract

Herbaceous and woody plants propagated in vitro are frequently affected by the redundant presence of various culture factors that lead to metabolic and morphological derangements. Anatomical, morphological and physiological anomalies in tissue cultured plants have been described by diverse terminology — vitrification, translucency, hyperhydration, succulency and glassiness. Although vitrification is a misused term because it refers to a physical and not a biological process, it is the most often used one, since it describes changes in the leaves, giving rise to a glassy appearance.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Ariel T (1987) The effect of culture condition on development and acclimatization of Philodendron ‘Burgundy’ and Dianthus caryophyllus (pp 1–96) MSc thesis, The Hebrew University of Jerusalem, Israel.

    Google Scholar 

  • Beauchesne G (1981) Les milieux minéraux utilisés en culture et leur incidence sur l’apparition de boutures d’aspect pathologique, C R Acad Agric Paris 67: 1389–1397.

    Google Scholar 

  • Blanke MM, Belcher AR (1989) Stomata of apple leaves cultured in vitro, Plant Cell, Tissue Organ Culture 19: 85–89.

    Article  Google Scholar 

  • Bornman CH, Vogelmann TC (1984) Effect of rigidity of gel medium on benzyladenine-induced adventitious bud formation and vitrification in in vitro Picea abies, Physiol Plant 61: 505–512.

    Article  CAS  Google Scholar 

  • Borkowska B, Michalczuk L (1987) The physiological disorders of sour cherry cultures: necrosis and vitrification, Acta Hort 212: 235–237.

    Google Scholar 

  • Boxus P, Quoirin M (1977) Comportement en pépinière d’arbres fruitiers issus de culture in vitro, Acta Hort 78: 373–378.

    Google Scholar 

  • Boxus P, Druart P, Brasseur E (1978) Rapport d’activités du Centre de Recherches de Gembloux, 126–127.

    Google Scholar 

  • Brainerd KE, Fuchigami LH (1981) Acclimatization of aseptically cultured apple plants to low-relative humidity, J Amer Soc Hort Sci 106: 515–518.

    Google Scholar 

  • Brainerd KE, Fuchigami LH (1982) Stomatal functioning of in vitro and greenhouse apple leaves in darkness, mannitol, ABA, CO2, J Expt Bot 33: 388–392.

    Article  CAS  Google Scholar 

  • Brainerd KE, Fuchigami LH, Kwiatkowski S, Clark CS (1981) Leaf anatomy and water stress of aseptically cultured ‘Pixy’ plum grown under different environments, HortScience 16: 173–175.

    Google Scholar 

  • Capellades M, Vanderschaeghe A, Lemuer R, Debergh P (1990) How important is photosynthesis in micropropagatuon? In: Sangwan RS, Sangwan-Norreel BS (Eds) The Impact of Biotechnology in Agriculture (in press) Kluwer Academic Pubis, Dordrecht.

    Google Scholar 

  • Conner LN, Conner AJ (1984) Comparative water loss from leaves of Solanum laciniatum plants cultured in vitro and in vivo, Plant Sci Letters 36: 241–246.

    Article  Google Scholar 

  • Cutter EG (1971) Control of leaf form. In: Plant Anatomy, Part 2: Organs (pp 179–191) Contemporary Biology, Eduard Arnold Ltd, London.

    Google Scholar 

  • Daguin F, Letouzé R (1985) Relations entre hypolignification et état vitreux chez Salix babylonica en culture in vitro. Rôle de la nutrition ammoniacale, Can J Bot 63: 324–326.

    Article  CAS  Google Scholar 

  • Daguin F, Letouzé R (1986) Ammonium-induced vitrification in cultured tissues, Physiol Plant 66: 94–98.

    Article  CAS  Google Scholar 

  • Debergh PC (1983) Effects of agar brand and concentration on the tissue culture medium, Physiol Plant 59: 270–276.

    Article  CAS  Google Scholar 

  • Debergh PC (1987) Improving micropropagation, IAPTC Newsletter 51: 2–10.

    Google Scholar 

  • Debergh PC, Harbaoui Y, Lemeur R (1981) Mass propagation of globe artichoke (Cynara scolymus): Evaluation of different hypotheses to overcome vitrification with special reference to water potential, Physiol Plant 53: 181–187.

    Article  Google Scholar 

  • Debergh PC, Maene LJ (1984) Pathological and physiological problems related to the in vitro culture of plants, Parasitica 40: 69–75.

    Google Scholar 

  • Debergh PC, Maene LJ (1985) Preparation of tissue cultured plants for rooting and establishment in vivo. In: Novak FJ, Havel T, Dolezel K (Eds) Proc Intl Symp Plant Tissue Culture Application to Crop Improvement (pp 487–495) Olomouc, Czechoslovakia.

    Google Scholar 

  • Delmer D (1987) Cellulose biosynthesis, Annu Rev Plant Physiol 38: 259–290.

    Article  CAS  Google Scholar 

  • Dencso I (1987) Factors influencing vitrification of carnation and conifers, Acta Hort 212: 167–176.

    Google Scholar 

  • De Proft MP, Van den Broek G, de Greef JA (1987) Involvement of ethylene in senescence and vitrification of in vitro cultured miniroses, Acta Hort 212: 217–222.

    Google Scholar 

  • Dolev E (1986) Hardening of tissue cultured fern and carnation plantlets, MSc thesis, The Hebrew University of Jerusalem, Israel.

    Google Scholar 

  • Donnelly DJ, Vidaver WE (1984a) Leaf anatomy of red raspberry transferred from in vitro culture to soil, J Amer Soc Hort Sci 109: 172–176.

    Google Scholar 

  • Donnelly DJ, Vidaver WE (1984b) Pigment content and gas exchange of red raspberry in vitro and ex vitro, J Amer Soc Hort Sci 109: 177–181.

    CAS  Google Scholar 

  • Donnelly DJ, Vidaver WE, Colbow K (1984) Fixation of CO2 in tissue cultured red raspberry prior to and after transfer to soil, Plant Cell Tissue Organ Culture 3: 313–317.

    Article  Google Scholar 

  • Earle ED, Langhans RW (1975) Carnation propagation from shoot tips cultured in liquid medium, HortScience 10: 608–610.

    Google Scholar 

  • Fabbri A, Sutter E, Dunston SK (1986) Anatomical changes in persistent leaves of tissue cultured strawberry plants after removal from culture, Scientia Hort 28: 331–337.

    Article  Google Scholar 

  • Fuchigami LH, Cheng TY, Soeldner A (1981) Abaxial transpiration and water loss in aseptically cultured plums, J Amer Soc Hort Sci 106: 519–522.

    Google Scholar 

  • Gaspar T, Kevers C (1985) Cobalt prevention of vitrification process in carnation, Plant Physiol 77 (Supp): 13.

    Google Scholar 

  • Gaspar T, Kevers C, Debergh P, Maene L, Paques M, Boxus P (1987) Vitrification: morphological, physiological and ecological aspects. In: Bonga JM, Durzan DJ (Eds) Cell and Tissue Culture in Forestry, Vol I (pp 152–166) Martinus Nijhoff Publ, Dordrecht, Holland.

    Google Scholar 

  • Grout BWW (1975) Wax development on leaf surface of Brassica oleracea botrytis cv Currawon regenerated from meristem culture, Plant Sci Lett 5: 401–405.

    Article  Google Scholar 

  • Grout BWW, Aston H (1977a) Transplanting of cauliflower plants regenerated from meristem culture I. Water loss and water transfer related to changes in leaf wax and to xylem regeneration, Hort Res 17: 1–7.

    Google Scholar 

  • Grout BWW, Aston H (1977b) Transplanting of cauliflower plants regenerated from meristem culture II. Carbon dioxide fixation and the development of photosynthetic ability, Hort Res 17: 65–71.

    Google Scholar 

  • Grout BWW, Aston H (1978) Modified leaf anatomy of cauliflower plantlets regenerated from meristem culture, Ann Bot 42: 993–995.

    Google Scholar 

  • Grout BWW, Price F (1987) The establishment of photosynthesis independence in strawberry cultures prior to transplanting. In: Ducaté G, Jacob M, Simeon A (Eds) Plant Micropropagation in Horticultural Industries (pp 55–60) Presses Universitaires, Liège, Belgium.

    Google Scholar 

  • Grout BWW, Donkin ME (1987) Photosynthetic activity of cauliflower meristem culture in vitro and at transplanting into soil, Acta Hort 212: 323–327.

    Google Scholar 

  • Hakkaart FA, Versluijs JMA (1983) Some factors affecting glassiness in carnation meristem tip cultures, Neth J Plant Path 89: 47–53.

    Article  Google Scholar 

  • Hegedus P, Phan CT (1983) Action de phénols sur les malformations observées chez les porte-greffes de pommiers M-26 et 0–3 cultivés in vitro, Rev Can Biol Exp 42: 33–38.

    CAS  Google Scholar 

  • Hegedus P, Phan CT (1987) Activities of 5 enzymes of the phenolic metabolism on rooted and acclimated vitreous plants in relation with phenolic treatments, Acta Hort 212: 211–216.

    Google Scholar 

  • Hughes KW (1981) In vitro ecology: exogenous factors affecting growth and morphogenesis in plant culture systems, Environ Exp Bot 21: 281–288.

    Article  CAS  Google Scholar 

  • Hussey G (1986) Problems and prospects in the in vitro propagation of herbaceous plants. In: Withers LA, Alderson PG (Eds) Plant Tissue Culture and its Agricultural Applications (pp 69–84) Butterworths, London.

    Google Scholar 

  • Jackson MB (1988) Aerenchyma formation in roots and leaves: regulation by oxygen shortage and ethylene. In: Proceedings NATO ISEP: Signals for Cell Seperation in Plants (Abstract p 46) Turin, Italy.

    Google Scholar 

  • John A (1986) Vitrification in Sitka spruce cultures. In: Withers LA, Alderson PG (Eds) Plant Tissue Culture and its Agricultural Applications (pp 167–174) Butterworths, London.

    Google Scholar 

  • Kauss H (1987) Some aspects of calcium dependent regulation in plant metabolism, Annu Rev Plant Physiol 38: 47–72.

    Article  CAS  Google Scholar 

  • Kevers C, Coumans M, Coumans-Gilles MF, Gaspar T (1984) Physiological and biochemical events leading to vitrification of plants cultured in vitro, Physiol Plant 61: 69–74.

    Article  CAS  Google Scholar 

  • Kevers C, Gaspar T (1985a) Soluble, membrane and wall peroxidases, phenylalanine ammonia-lyase, and lignin changes in relation to vitrification of carnation tissues cultured in vitro, J Plant Physiol 118: 41–48.

    CAS  Google Scholar 

  • Kevers C, Gaspar T (1985b) Vitrification of carnation in vitro: changes in ethylene production, ACC level and capacity to convert ACC to ethylene, Plant Cell Tissue Organ Culture 4: 215–223.

    Article  CAS  Google Scholar 

  • Kevers C, Gaspar T (1986) Vitrification of carnation in vitro: changes in water content, extracellular space, air volume, and ion levels, Physiol Vég 24: 647–653.

    CAS  Google Scholar 

  • Kevers C, Prat R, Gaspar T (1987) Vitrification of carnation in vitro: changes in cell wall mechanical properties, cellulose and lignin content, Plant Growth Regulation 5: 59–66.

    Article  CAS  Google Scholar 

  • Koshuchowa S, Bottcher I, Zoglauer K, Göring H (1988) Avoidance of vitrification of in vitro cultured plants. In: Proceedings 6th Congress of FESPP (Abstract p 1418) Split, Yoguslavia.

    Google Scholar 

  • Kozai T, Oki H, Fujiwara K (1987) Effects of CO2 enrichment and sucrose concentration under high photosynthetic photon fluxes on growth of tissue-cultured Cymbidium plantlets during the preparation stage. In: Ducaté G, Jacob M, Simeon A (Eds) Plant Micropropagation in Horticultural Industries (pp 47–54) Presses Universitaires, Liège, Belgium.

    Google Scholar 

  • Kozai T, Iwanami Y (1988) Effects of CO2 enrichments and sucrose concentration under high photosynthetic photon flux on the tissue-cultured plantlet of Carnation (Dianthus caryophyllus) during the preparation stage, J Jap Soc Hort Sci 57: 255–264.

    Article  Google Scholar 

  • Kreutmeier C, Gebhardt K, Paul L, Feucht W (1984) The effect of MgSO4 and CaCl2 on regeneration of shoot tip cultures of Prunus cerasus in vitro, Gartenbauwissenschaft 49: 204–212.

    CAS  Google Scholar 

  • Lee N, Wetzstein HY, Sommer HE (1985) Effect of quantum flux density on photosynthesis and chloroplast ultrastructure in tissue-cultured plantlets and seedlings of Liquidambar styraciflua L towards improved acclimatization and field survival, Plant Physiol 78: 637–641.

    Article  PubMed  CAS  Google Scholar 

  • Leonhardt W, Kandeler R (1987) Ethylene accumulation in culture vessels — a reason for vitrification, Acta Hort 212: 223–229.

    Google Scholar 

  • Leshem B (1983a) Growth of carnation meristems in vitro: anatomical structure of abnormal plantlets and the effect of agar concentration in the medium on their formation, Ann Bot 52: 413–415.

    Google Scholar 

  • Leshem B (1983b) The carnation succulent plantlet — a stable teratological growth, Ann Bot 52: 873–876.

    Google Scholar 

  • Leshem B, Shalev DP, Izhar S (1988a) Cytokinin as an inducer of vitrification in melon, Ann Bot 61: 255–260.

    CAS  Google Scholar 

  • Leshem B, Werker E, Shalev PD (1988b) The effect of cytokinins on vitrification in melon and carnation, Ann Bot 62: 271–276.

    CAS  Google Scholar 

  • Letouzé R, Daguin F (1983) Manifestation spontanée et aléatoire d’une croissance anormale en culture in vitro. Recherche de marqueurs métaboliques, Rev Can Biol Exp 42: 23–28.

    Google Scholar 

  • Letouzé R, Daguin F (1987) Control of vitrification and hypolignification process in Salix babylonica cultured in vitro, Acta Hort 212: 185–191.

    Google Scholar 

  • Maene L, Debergh P (1985) Liquid medium additions to established tissue cultures to improve elongation and rooting in vivo, Plant Cell Tissue Organ Culture 5: 23–34.

    Article  CAS  Google Scholar 

  • Maene L, Debergh P (1987) Optimalisation of the transfer of tissue cultured shoots to in vivo conditions, Acta Hort 212: 335–348.

    Google Scholar 

  • Marin JA, Gella R, Herrero M (1988) Stomatal structure and functioning as a response to environmental changes in acclimatized micropropagated Prunus cerasus L., Ann Bot 62: 663–670.

    Google Scholar 

  • Mele E, Messeguer J, Camprubi P (1982) Effects of ethylene on carnation expiants grown in sealed vessels. In: Fujiwara A (Ed) Plant Tissue Culture 1982 (pp 69–70) Proc IAPTC Tokyo, Japan.

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol Plant 15: 473–497.

    Article  CAS  Google Scholar 

  • Orlikowska T (1987) Vitrification problem in the in vitro culture of fruit tree rootstocks, Acta Hort 212: 239–244.

    Google Scholar 

  • Palevitz BA (1981) The structure and development of stomatal cells. In: Jarvis PG, Mansfield TA (Eds) Stomata Physiology (pp 1–230) Cambridge University Press, Cambridge.

    Google Scholar 

  • Paques M, Boxus P (1987a) Vitrification: review of literature, Acta Hort 212: 155–166.

    Google Scholar 

  • Paques M, Boxus P (1987b) A model to learn ‘vitrification’, the rootstock apple M.26 present results, Acta Hort 212: 193–210.

    Google Scholar 

  • Paques M, Boxus P (1987c) Vitrification: a phenomenon related to tissue water content, Acta Hort 212: 245–252.

    Google Scholar 

  • Paques M, Boxus P, Dulos M (1987) Vitrification: an inducible and reversible phenomenon, Acta Hort 212: 253–258.

    Google Scholar 

  • Pasqualetto PL, Zimmerman RH, Fordham I (1986) The influence of gelling agent and growth regulator concentrations on vitrification of apple cultivars in vitro, J Amer Soc Hort Sci 111: 976–980.

    CAS  Google Scholar 

  • Pasqualetto PL, Zimmerman RH, Fordham I (1988) The influence of cation and gelling agent concentration on vitrification of apple cultivars in vitro, Plant Cell Tissue Organ Culture 14: 31–40.

    Article  CAS  Google Scholar 

  • Phan CT, Letouzé R (1983) A comparative study of chlorophyll, phenolic and protein contents and of hydroxycinnamate: CoA ligase activity of normal and vitreous plants (Prunus avium L.) obtained in vitro, Plant Sci Lett 31: 323–327.

    Article  CAS  Google Scholar 

  • Quoirin M, Lepoivre P (1977) Etude de milieux adaptés aux cultures in vitro de Prunus, Acta Hort 78: 437–442.

    Google Scholar 

  • Reuther G (1988) Comparative anatomical and physiological studies with ornamental plants under in vitro and greenhouse conditions, Acta Hort 226: 91–98.

    Google Scholar 

  • Riffaud JL, Cornu D (1981) Utilisation de la culture in vitro pour la multiplication de merisiers adultes (Prunus avium L.) sélectionnés en fôret, Agronomie 1: 633–640.

    Article  Google Scholar 

  • Rugini E, Tarini P, Rossodivita ME (1987) Control of shoot ‘vitrification’ of almond and olive grown in vitro, Acta Hort 212: 177–183.

    Google Scholar 

  • Rugini E, Verma DC (1983) Micropropagation of difficult-to-propagte almond (Prunus amygdalus Batsch) cultivars, Plant Sci Lett 28: 273–281.

    Google Scholar 

  • Short KC, Wardle K, Grout BWW, Simpkins I (1985) In vitro physiology and acclimatization of aseptically cultured plantlets. In: Novak FJ, Havel T, Dolezel K (Eds) Proc Intl Symp Plant Tissue Culture Application for Crop Improvement (pp 475–486) Olomouc, Czechoslovakia.

    Google Scholar 

  • Short KC, Warburton J, Roberts AV (1987) In vitro hardening of cultured cauliflower and chrysanthemum plantlets to humidity, Acta Hort 212: 329–334.

    Google Scholar 

  • Sutter EG (1984) Chemical composition of epicuticular wax in cabbage plants grown in vitro, Can J Bot 62: 74–77.

    Article  CAS  Google Scholar 

  • Sutter EG (1985) Morphological, physical and chemical characteristics of epicuticular wax on ornamental plants regenerated in vitro, Ann Bot 55: 321–329.

    Google Scholar 

  • Sutter EG, Langhans RW (1979) Epicuticular wax formation on carnation plantlets regenerated from shoot tip culture, J Amer Soc Hort Sci 104: 493–496.

    Google Scholar 

  • Sutter EG, Langhans RW (1982) Formation of epicuticular wax and its effect on water loss in cabbage plants regenerated from shoot-tip culture, Can J Bot 60: 2896–2902.

    Article  Google Scholar 

  • Vanderschaeghe AM, Debergh PC (1987) Technical aspects of the control of the relative humidity in tissue culture containers. In: Ducaté G, Jacob M, Simeon A (Eds) Plant Micropropagation in Horticultural Industries (pp 68–76) Presses Universitaires, Liège, Belgium.

    Google Scholar 

  • Van Huystee, RS (1987) Some molecular aspects of plant peroxidase biosynthetic studies, Annu Rev Plant Physiol 38: 205–219.

    Article  Google Scholar 

  • Vieitez AM, Ballester A, San-Jose MC, Vieitez E (1985) Anatomical and chemical studies of vitrified shoots of chestnut regenerated in vitro, Physiol Plant 65: 177–184.

    Article  CAS  Google Scholar 

  • Vieitez AM, Ballester A, Vieitez E (1987) Vitrification in chestnut shoots regenerated in vitro, Acta Hort 212: 231–234.

    Google Scholar 

  • Vieth J, Morisset C, Lamand M (1983) Histologie de plantules vitreuses de Pyrus malus cv M-26 et de Pelargonium peltatum cv Chester Frank issues de la culture in vitro, Rev Can Biol Exp 42: 29–32.

    Google Scholar 

  • von Arnold S, Eriksson T (1984) Effect of agar concentration on growth and anatomy of adventitious shoots of Picea abies (L) Karst, Plant Cell Tissue Organ Culture 3: 257–264.

    Article  Google Scholar 

  • Wardle K, Short KC (1983) Stomatal responses of in vitro cultured plantlets I. Responses in epidermal strips of Chrysanthemum to environmental factors and growth regulators, Biochem Physiol Pflanzen 178: 619–624.

    CAS  Google Scholar 

  • Wardle K, Dixon PA, Simpkin I (1981) Sodium accumulation by leaves of cauliflower plantlets and the effect on the mode of plant formation, Ann Bot 47: 653–659.

    CAS  Google Scholar 

  • Wardle K, Dobbs EB, Short KC (1983) In vitro acclimatization of aseptically cultured plantlets to humidity, J Amer Soc Hort Sci 108: 386–389.

    Google Scholar 

  • Wardle K, Quinlan A, Simpkins I (1979) Abscisic acid and the regulation of water loss in plantlets of Brassica oleraea L var botrytis regenerated through apical meristem culture, Ann Bot 43: 745–752.

    CAS  Google Scholar 

  • Werker E, Leshem B (1987) Structural changes during vitrification of carnation plantlets, Ann Bot 59: 377–385.

    Google Scholar 

  • Wetzstein HY, Sommer HE (1982) Leaf anatomy of tissue cultured Liquidambar styraciflua (Hamamelidaceae) during acclimatization, Amer J Bot 69: 1579–1586.

    Article  Google Scholar 

  • Wetzstein HY, Sommer HE (1983) Scanning electron microscopy of in vitro-cultured Liquidambar styraciflua plantlets during acclimatization, J Amer Soc Hort Sci 108: 475–480.

    Google Scholar 

  • Wetzstein HY, Sommer HE (1985) Effects of quantum flux density on photosynthesis and chloroplast ultrastructure in tissue-cultured plantlets and seedlings of Liquidambar styraciflua L. towards improved acclimatization and field survival, Plant Physiol 78: 637–641.

    Article  PubMed  Google Scholar 

  • Yang SF, Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants, Annu Rev Plant Physiol 35: 155–189.

    Article  CAS  Google Scholar 

  • Ziv M (1979) Transplanting Gladiolus plants propagated in vitro, Scientia Hort 11: 257–260.

    Article  Google Scholar 

  • Ziv M (1986) In vitro hardening and acclimatization of tissue culture plants. In: Withers LA, Alderson PG (Eds) Plant Tissue Culture and its Agricultural Applications (pp 187–196) Butterworths, London.

    Google Scholar 

  • Ziv M (1989) Enhanced shoot and cormlet proliferation in liquid cultured gladiolus buds by growth retardants, Plant Cell Tissue Organ Culture 17: 101–110.

    Article  CAS  Google Scholar 

  • Ziv M, Gadasi G (1986) Enhanced embryogenesis and plant regeneration from cucumber (Cucumis sativus L.) callus by activated charcoal in solid/liquid double-layer cultures, Plant Sci 47: 115–122.

    Article  CAS  Google Scholar 

  • Ziv M, Ariel T (1988) The relationship between cell wall deformity and stomatal malfunction in the leaves of carnation in vitro. In: Proc Intl Soc Plant Molecular Biol, Congress, Jerusalem: 425.

    Google Scholar 

  • Ziv M, Ariel T (1990) The effect of culture condition on vitrification and stomatal cell wall deformation in leaves of carnation plants in vitro, Plant Sci (In press).

    Google Scholar 

  • Ziv M, Lilien-Kipnis H (1990) Gladiolus. In: Ammirato PV, Evans DA, Sharp WR, Bajaj YPS (Eds) Handbook of Plant Cell Cultures Vol 5 (pp 461–478) McGraw-Hill Company, NY.

    Google Scholar 

  • Ziv M, Meir G, Halevy AH (1981) Hardening carnation plantlets regenerated from shoot tips cultured in vitro, Environ Exp Bot 21: 423.

    Google Scholar 

  • Ziv M, Meir G, Halevy AH (1983) Factors influencing the production of hardened glaucous carnation plantlets in vitro, Plant Cell Tissue Organ Culture 2: 55–60.

    Article  Google Scholar 

  • Ziv M, Schwarts A, Fleminger D (1987a) Malfunctioning stomata in vitreous leaves of carnation (Dianthus caryophyllus) plants propagated in vitro; implications for hardening, Plant Sci 52: 127–134.

    Article  CAS  Google Scholar 

  • Ziv M, Schwarts A, Fleminger D (1987b) Hardening aspects of micropropagated carnation plants having malfunctioning stomata. In: Ducaté G, Jacob M, Simeon A (Eds) Plant Micro-propagation in Horticultural Industries (pp 47–54) Presses Universitaires, Liège, Belgium.

    Google Scholar 

  • Zuccherelli G (1979) Moltiplicazione in vitro dei portainnesti clonali del pesco, Frutticoltura 41: 15–20.

    Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Kluwer Academic Publishers

About this chapter

Cite this chapter

Ziv, M. (1991). Vitrification: morphological and physiological disorders of in vitro plants. In: Debergh, P.C., Zimmerman, R.H. (eds) Micropropagation. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2075-0_4

Download citation

  • DOI: https://doi.org/10.1007/978-94-009-2075-0_4

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-0819-5

  • Online ISBN: 978-94-009-2075-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics