Abstract
Yeasts and aerobic fungi have long been known to be normal inhabitants of the rumen (Clarke and DiMenna, 1961; Sivers, 1962a, b; Lund, 1974) but most species isolated are considered to be transient and non-functional, entering the rumen with the feed. Some aerobic fungi are capable of growth under anaerobic conditions, and Brewer et al. (1972) concluded that Aspergillus fumigatus, Mucor rouxii and Sporormia minima, which are implicated as causative agents in ovine ill-thrift, could survive in the rumen. Two other groups of fungi are now known to occur, one group parasitic upon ciliate protozoa, the other saprophytic on plant tissues.
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References
Akin, D. E. (1987). Use of chitinase to determine rumen fungi with plant tissues in vitro. Appl. Environ. Microbiol., 53, 1955–8.
Akin, D. E. (1994). Ultrastructure of plant cell-walls degraded by anaerobic fungi. In Anaerobic Fungi: Biology, Ecology and Function, ed. D. O. Mountfort and C. G. Orpin. Marcel Dekker, New York, pp. 169–90.
Akin, D. E. and Rigsby, L. L. (1985). Influence of phenolic acids on rumen fungi. Agron. 77, 180–2.
Akin, D. E., Gordon, G. L. R. and Hogan, J. P. (1983). Rumen bacterial and fungal degradation ofDigitaria pentzii grown with and without sulphur. Appl. Environ. Microbiol, 46, 738–48.
Akin, D. E., Borneman, W. S. and Lyon, C. E. (1990). Degradation of leaf blades and stems by monocentric and polycentric isolates of ruminal fungi. Anim. Fd Sci. Technol., 31, 205–22.
Argyle, J. L. E. and Douglas, L. (1989). Chitin as a fungal marker. In The Roles of Protozoa and Fungi in Ruminant Digestion, ed. J. V. Nolan, R. A. Leng and D. I. Demeyer. Perambul Books, Armidale, Australia, pp. 289–90.
Aylward, J. H., Xue, G. P., Simpson, G. D. and Orpin, C. G. (1993). Cellobiohydrolase (CBH) from Neocallimastix patriciarum: a membrane-associated complex? Proceedings of the XVII International Grasslands Congress, pp. 1222–4.
Barichievich, E. M. and Calza, R. E. (1990a). Supernatant protein and cellulase activities of the anaerobic rumen fungus Neocallimastix frontalis EB188. Appl. Environ. Microbiol., 56, 43–8.
Barichievich, E. M. and Calza, R. E. (1990b). Media carbon induction of extracellular cellulase activities inNeocallimastix frontalis EB188. Curr. Microbiol., 20, 265–71.
Barr, D. J. S. (1981). The phylogenetic and taxonomic implications of flagellar rootlet morphology among zoosporic fungi.Biosystems, 15, 359–70.
Barr, D. J. S. (1988). How modern systematics relates to the rumen fungi. Biosystems, 21, 355–6.
Barr, D. J. S. (1989). An outline for the reclassification of the Chytridiales and for a new order, the Spizellomycetales. Can. J. Bot., 58, 2380–94.
Barr, D. J. S., Kudo, H., Jackober, K. D. and Cheng, K-J. (1989). Morphology and development of rumen fungi: Neocallimastix sp., Piromyces communis and Orpinomyces bovis, gen. nov., sp. nov. Can. J. Bot., 67, 2815–24.
Bauchop, T. (1979). Rumen anaerobic fungi of cattle and sheep. Appl. Environ. Microbiol., 38, 148–58.
Bauchop, T. (1980). Scanning electron microscopy in the study of microbial digestion of plant fragments in the gut. In Contemporary Microbial Ecology, ed. D. C. Ellwood, J. N. Hedger, M. J. Latham, et al. Academic Press, London, pp. 305–26.
Bauchop, T. (1981). The anaerobic fungi in rumen fibre digestion. Agric. Environ., 6, 338–48.
Bauchop, T. (1983). The gut anaerobic fungi: colonizers of dietary fibre. In Fibre in Human and Animal Nutrition, ed. G. Wallace and L. Bell. Royal Society of New Zealand, Wellington, pp. 143–8.
Bauchop, T. (1986). Rumen anaerobic fungi and the utilization of fibrous feeds. Rev. Rural Sei., 6, 118–23.
Bauchop, T. and Mountfort, D. O. (1981). Cellulose fermentation by a rumen anaerobic fungus in both the absence and presence of rumen methanogens. Appl. Environ. Microbiol., 42, 1103–10.
Beguin, P., Millet, J., Chavaux, E. et al. (1989). Genetics of bacterial cellulases. In Enzyme Systems for Lignocellulose Degradation, ed. M. P. Coughlan. Elsevier Applied Science, London, pp. 57–72.
Bernalier, A., Fonty, G. and Gouet, Ph. (1988). Dégradation et fermentation de la cellulose parNeocallimastix sp. MCH3 seul ou associé a quelques espèces bactériennes du rumen. Reprod. Nutr. Develop., 28 (Suppl. 1), 75–6.
Bernalier, A., Fonty, G. and Gouet, Ph. (1991). Cellulose degradation by two rumen anaerobic fungi in monoculture or in coculture with rumen bacteria. Anim. Fd Sei. Technol., 32, 131–6.
Bernalier, A., Fonty, G., Bonnemoy, F. and Gouet, Ph. (1992). Degradation and fermentation of cellulose by the rumen anaerobic fungi in axenic cultures or in association with cellulolytic bacteria. Curr. Microbiol, 25, 143–8.
Bernalier, A., Fonty, G., Bonnemoy, F. and Gouet, Ph. (1993). Effect ofEubacterium limosum, a ruminai hydrogenotrophic bacterium, on the degradation and fermentation of cellulose by 3 species of rumen anaerobic fungi. Reprod. Nutr. Develop., 33, 577–84.
Bernalier, A., Fonty, G., Bonnemoy, F. and Gouet, Ph. (19936). Inhibition of the cellulolytic activity of Neocallimastix frontalis by Ruminococcus flavefaciens. J. Gen. Microbiol., 139, 873–80.
Bernard-Vailhé, M. A., Besle, J. M. and Doré, J. (1995). Transformation of 14C lignin-labeled cell walls of wheat by Syntrophococcus sucromutans, Eubacterium oxidoreducens and Neocallimastix frontalis. Appl. Environ. Microbiol., 61, 379–81.
Billon-Grand, G., Liol, J. B., Breton, A. et al. (1991). DNA of some anaerobic rumen fungi: G + C content determination. FEMS Microbiol. Lett., 82, 267–70.
Black, G. W., Hazlewood, G. P., Xue, G.-P. et al. (1994). Xylanase B from Neocallimastix patriciarum contains a non-catalytic 455-residue linker sequence comprised of 57 repeats of an octapeptide. Biochem. J., 299, 381–7.
Body, D. R. and Bauchop, T. (1985). Lipid composition of an obligately anaerobic fungus Neocallimastix frontalis isolated from a bovine rumen. Can. J. Microbiol., 31, 463–6.
Borneman, W. S. and Akin, D. E. (1990). Lignocellulose degradation by rumen fungi and bacteria: ultrastructure and cell-wall degrading enzymes. In Microbial and Plant Opportunities to Improve Lignocellulose Utilization by Ruminants, ed. D. E. Akin, L. G. Ljungdahl, J. R. Wilson and P. J. Harris. Elsevier, New York, pp. 225–49.
Borneman, W. S., Hartley, R. D., Morrison, W. H. et al. (1990). Feruloyl and /7-coumaroyl esterase from anaerobic fungi in relation to plant cell wall degradation. Appl. Environ. Microbiol., 33, 345–51.
Bowman, B. H., Taylor, J. W, Brownlee, A. G. et al. (1992). Molecular evolution of the fungi: relationship of theBasidomyces, Ascomyces and Chytridiomyces. Mol. Biol. Evoi, 9, 285–96.
Braune, R. (1913). Untersuchungen über die in Wiederkauermagen vorkommenden protozoen. Arch. Protist., 32, 111–70.
Breton, A., Bernalier, A., Bonnemoy, F. et al. (1989). Morphology and metabolic characterization of a new spieces of strictly anaerobic rumen fungus: Neocallimastix joyonii. FEMS Microbiol. Lett., 58, 309–14.
Breton, A., Bernalier, A., Dusser, M.etal. (1990). Anaeromyces mucronatus nov. gen., nov. sp. A new strictly anaerobic rumen fungus with polycentric thallus. FEMS Microbiol Lett., 70, 177–82.
Breton, A., Dusser, M., Gaillard-Martinie, B. et al. (1991). Piromyces rhizinflata, a species of strictly anaerobic fungus from feces of the Saharan ass: a morphological, metabolic and ultrastructural study. FEMS Microbiol. Lett., 82, 1–8.
Breton, A., Dusser, M., Gaillard-Martinie, B. and Guillot, J. (1994). Cell wall composition and detection of anaerobic rumen fungi in vitro, using fluorescent lectins. In Microorganisms in Ruminant Nutrition, ed. R. A. Prins and C. S. Stewart. Nottingham University Press, Nottingham, pp. 167–78.
Brewer, D., Duncan, J. M., Safe, S. and Taylor, A. (1972). Ovine ill-thrift in Nova Scotia. 4. The survival at low oxygen partial pressure of fungi isolated from the contents of the ovine rumen. Can. J. Microbiol, 18, 1119–28.
Brownlee, A. G. (1986). Properties of chitin synthetase from a rumen anaerobic fungus. Abstracts of XIV International Congress of Microbiology, P. M4–3.
Brownlee, A. G. (1986b). Genome organization in an anaerobic fungus from sheep rumen. Abstracts of XIV International Congress of Microbiology, P. G1–19.
Brownlee, A. G. (1989). Remarkably AT-rich genomic DNA from the anaerobic fungus Neocallimastix. Nucleic Acids Res., 17, 1327–35.
Brownlee, A. G. (1994). The nucleic acids of rumen fungi. In Anaerobic Fungi: Biology, Ecology and Function, ed. D. O. Mountfort and C. G. Orpin. Marcel Dekker, New York, pp. 241–56.
Calza, R. E. (1990). Regulation of protein and cellulase excretion in the rumen fungus Neocallimastix frontalis EB 188.Curr. Microbiol, 21, 109–15.
Calza, R. E. (1991a). Cellulase fromNeocallimastix frontalis EB188 synthesized in the presence of protein glycosylation inhibitors: measurement of protein molecular weights and isoelectric focusing values. Appl Microbiol Technol., 35, 748–52.
Calza, R. E. (1991 b). Nascent synthesis and secretion of cellobiase in Neocallimastix frontalis EB188. Curr. Microbiol., 23, 175–80.
Chen, M. and Wolin, M. J. (1977). Influence of methane production byMethanobacterium ruminantium on the fermentation of glucose and lactate by Selenomonas ruminantium. Appl Environ. Microbiol, 34, 756–9.
Chen, M. and Wolin, M. J. (1979). Effect of monensin and lasalocid-sodium on the growth of methanogenic and rumen saccharolytic bacteria. Appl Environ. Microbiol, 38, 72–7.
Clarke, R. T. J. and DiMenna, M. E. (1961). Yeasts from the bovine rumen. J. Gen. Microbiol, 25, 113–17.
Coleman, G. S. (1989). Protozoal-bacterial interactions of the rumen. In The Roles of Protozoa and Fungi in Ruminant Digestion, ed. J. V. Nolan, R. A. Leng and D. I. Demeyer. Penambul Books, Armidale, Australia, pp. 13–27.
Coughlan, M. P. (1990). Cellulose degradation by fungi. In Microbial Enzymes and Biotechnology, ed. W. M. Fogarty and C. T. Kelly. Elsevier, Amsterdam, pp. 1–36.
Davies, D. R., Theodorou, M. K., Brooks, A. E. and Trinci, A. P. J. (1993). Influence of drying on the survival of anaerobic fungi in rumen digesta and faeces of cattle. FEMS Microbiol Lett., 106, 59–64.
Davies, D. R., Theodorou, M. K., Lawrence, M. I. and Trinci, A. P. J. (1993b). Distribution of anaerobic fungi in the digestive tract of cattle and their survival in faeces. J. Gen. Microbiol., 139, 59–64.
DeBlois, S. and Wiegle, J. (1990). Hemicellulases in lignocellulose degradation. In Microbial and Plant Opportunities to Improve Lignocellulose Utilization by Ruminants, ed. D. E. Akin, L. G. Ljungdahl, J. R. Wilson and P. J. Harris. Elsevier, New York, pp. 275–87.
Demeyer, D. I. (1981). Rumen microbes and digestion of plant cell walls. Agric. Environ., 6, 295–337.
Doré, J. and Stahl, D. A. (1991). Phylogeny of anaerobic rumen chytridiomycetes inferred from small subunit ribosomal RNA sequence comparison. Can. J. Bot., 69, 1964–71.
Durand, R., Reymond-Cotton, P., Fischer, M. et al. (1994). Cloning and expression of genes encoding enzymes of the glycolytic pathway inNeocallimastix frontalis. In Microorganisms in Ruminant Nutrition, ed. R. A. Prins and C. S. Stewart. Nottingham University Press, Nottingham, pp. 137–51.
Engels, F. M. and Brice, R. E. (1985). A barrier covering lignified cell walls of barley straw that resists access by rumen micro-organisms. Curr. Microbiol., 12, 217–24.
Fonty, G. and Joblin, K. N. (1991). Rumen anaerobic fungi: their role and interactions with rumen microorganisms in relation to fibre digestion. In Physiological Aspects of Digestion and Metabolism in Ruminants, ed. T. Tsuda, Y. Sasaki and R. Kawashima. Academic Press, New York, pp. 655–80.
Fonty, G., Gouet, Ph., Jouany, J. P. and Senand, J. (1987). Establishment of the microflora and anaerobic fungi in the rumen of lambs. J. Gen. Microbiol., 133, 1835–43.
Fonty, G., Gouet, Ph. and Santé, V. (1988). Influence d’une bactérie méthanogène sur l’activité cellulolytique et le métabolisme de deux espèces de champignons cellulolytique du rumen in vitro. Reprod. Nutr. Dev., 28, 133–4.
Fonty, G. and Grenet, E. (1994). Effects of diet on the fungal population of the digestive tract of ruminants. In Anaerobic Fungi: Biology, Ecology and Function, ed. D. O. Mountfort and C. G. Orpin. Marcel Dekker, New York, pp. 229–39.
Garcia-Campayo, V. and Wood, T. M. (1993). Purification and characterisation of a p-D-xylosidase from the rumen anaerobic fungus Neocallimastix frontalis. Carbohydrate Res., 242, 229–45.
Gay, L., Hebraud, M., Girard, V. and Fevre, M. (1989). Chitin synthase activity from Neocallimastix frontalis, an anaerobic rumen fungus. J. Gen. Microbiol., 135, 279–83.
Gilbert, H. J., Hazlewood, G. P., Laurie, J. I. et al. (1992). Xylanase A from the rumen anaerobic fungus Neocallimastix patriciarum contains two homologous catalytic domains. Mol. Microbiol., 6, 2065–72.
Gilkes, N. R., Henrissat, B., Kilburn, D. G. et al. (1991). Domains in microbial β-l,4-glucanases: sequence conservation, function, and enzyme families.Microbiol. Rev., 55, 303–15.
Gold, J. J., Heath, I. B. and Bauchop, T. (1988). Ultrastructural description of a new chytrid genus of caecum anaerobe, Caecomyces equi gen. nov. Biosystems, 21, 403–15.
Gordon, G. L. R. (1986). The potential for manipulation of rumen fungi. Rev. Rural Sci., 6, 124–8.
Gordon, G. L. R. and Ashes, J. R. (1984). In vitro digestion of wheat straw by different rumen anaerobic fungi. Can. J. Anim. Sci., 64 (Suppl.), 156–7.
Gordon, G. L. R. and Phillips, M. W. (1989). Comparative fermentation properties of anaerobic fungi from the rumen. In The Roles of Protozoa and Fungi in Ruminant Digestion, ed. J. V. Nolan, R. A. Leng and D. I. Demeyer. Perambul Books, Armidale, pp. 127–38.
Gordon, G. L. R. and Phillips, M. W. (1992a). Extracellular pectin lyase produced by Neocallimastix sp. LM1, a rumen anaerobic fungus. In Proceedings, 3rd International Symposium on Nutrition of Herbivores, ed. M. W. Zahari, Z. A. Tajuddin, N. Abdullah and H. K. Wong. Serdang, Malaysian Society of Animal Production, p. 27.
Gordon, G. L. R. and Phillips, M. W. (1992b). Extracellular pectic lyase produced by Neocallimastix LM1, a rumen anaerobic fungus. Lett. Appl. Microbiol., 15, 113–15.
Gordon, G. L. R. and Phillips, M. W. (1993). Removal of anaerobic fungi from the rumen of sheep by chemical treatment and the effect on feed consumption and in vivo fibre digestion. Lett. Appl. Microbiol., 17, 220–3.
Greening, R. C. and Leedle, J. A. Z. (1989). Enrichment and isolation of Acetitomaculum ruminis gen. nov., sp. nov., acetogenic bacteria from the bovine rumen. Arch. Microbiol., 152, 399–406.
Grenet, E. and Barry, P. (1988). Colonization of thick-walled plant tissues by anaerobic fungi. Anim. Fd Sci. Technol., 19, 25–31.
Guilati, S. K., Ashes, J. R., Gordon, G. L. R. et al. (1989). Nutritional availability of amino acids from the rumen anaerobic fungus Neocallimastix LM1 in sheep. J. Agric. Sci. (Camb.), 113, 383–7.
Hawksworth, D. L., Kirk, P. M., Sutton, B. C. and Pegler, D. N. (1995). Chytridiomycota. In Ainsworth and Bisby’s Dictionary of the Fungi. CAB International, Wallingford, p. 89.
Heath, I. B. and Bauchop, T. (1985). Mitosis and the phylogeny of the genus Neocallimastix. Can. J. Bot, 63, 1595–604.
Heath, I. B., Bauchop, T. and Skipp, R. A. (1983). Assignment of the rumen anaerobe Neocallimastix frontalis to the Spizellomycetales (Chytridiomycetes) on the basis of its polyflagellate zoospore ultrastructure. Can. J. Bot., 61, 295–307.
Heath, I. B., Kaminsky, J. and Bauchop, T. (1986). Basal body loss during fungal zoospore encystment: evidence against centriole autonomy. J. Cell Sci., 83, 135–40.
Hebraud, M. and Fevre, M. (1988). Characterization of glycoside polysaccharide hydrolases secreted by the rumen fungi Neocallimastix frontalis, Sphaeromonas communis and Piromonas communis. J. Gen. Microbiol., 134, 1112–29.
Hebraud, M. and Fevre, M. (1990). Purification and characterization of an extracellular β-xylosidase from the rumen fungus Neocallimastix frontalis. FEMS Microbiol. Lett., 72, 11–16.
Henderson, C. (1980). The influence of extracellular hydrogen on the metabolism of Bacteroides ruminicola, Anaerovibrio lipolytica and Selenomonas ruminantium. J. Gen. Microbiol, 119, 485–91.
Ho, Y. W., Abdullah, N. and Jalaludin, S. (1988). Penetrating structures of anaerobic rumen fungi in cattle and swamp buffalo. J. Gen. Microbiol., 134, 177–81.
Ho, Y. W., Abdullah, N. and Jalaludin, S. (1988b). Colonization of guinea grass by anaerobic rumen fungi in swamp buffalo and cattle. Anim. Fd Sci. Technol, 22, 161–72.
Ho, Y. W., Bauchop, T., Abdullah, N. and Jalaludin, S. (1990). Ruminomyces elegans gen. et sp. nov. a polycentric anaerobic rumen fungus from cattle.Mycotaxon, 38, 397–405.
Ho, Y. W., Barr, D. J. S., Abdullah, N. et al. (1993a). Neocallimastix variabilis, a new species of anaerobic fungus from the rumen of cattle. Mycotaxon, 46, 241–58.
Ho, Y. W., Barr, D. J. S., Abdullah, N. et al. (1993b). Anaeromyces, an earlier name for Ruminomyces. Mycotaxon, 47, 283–4.
Ho, Y. W., Barr, D. J. S., Abdullah, N. et al. (1993c). A new species ofPiromyces from the rumen of deer in Malaysia. Mycotaxon, 47, 285–93.
Ho, Y. W., Barr, D. J. S., Abdullah, N. et al. (1993d). Piromyces spiralis a new species of anaerobic fungus from the rumen of goat. Mycotaxon, 48, 59–68.
Hodrova, B. and Kopecny, J. (1996). Interactions of the rumen chitinolytic bacterium Clostridium tertium with anaerobic fungi. Ann Zootech (in press).
Hodrova, B., Kopecny, J. and Petr, O. (1995). Interaction of the rumen fungusOrpinomyces joyonii with Megasphaera elsdenii and Eubacterium limosum. Lett. Appl. Microbiol, 21, 34–7.
Hungate, R. E. (1966). The Rumen and its Microbes. Academic Press, New York.
Irvine, H. L. and Stewart, C. S. (1991). Interactions between anaerobic cellulolytic bacteria and fungi in the presence of Methanobrevibacter smithii. Lett. Appl Microbiol, 12, 62–4.
Jensen, E. H. C. and Hammond, D. M. (1964). A morphological study of trichomonads and related flagellates from the bovine digestive tract. J. Protozool., 11, 386–94.
Joblin, K. N. (1981). Isolation, enumeration and maintenance of rumen anaerobic fungi in roll tubes. Appl Environ. Microbiol, 42, 1119–22.
Joblin, K. N. (1988). Physical disruption of plant fibre by rumen fungi of the Sphaeromonas group. In The Roles of Protozoa and Fungi in Ruminant Digestion, ed. J. V. Nolan, R. A. Leng and D. I. Demeyer. Penambul Books, Armidale.
Joblin, K. N. (1990). Bacterial and protozoal interactions with ruminal fungi. In Microbial and Plant Opportunities to Improve Lignocellulose Utilisation by Ruminants, ed. D. E. Akin, L. G. Ljungdahl, J. R. Wilson and P. J. Harris. Elsevier, New York, pp. 311–24.
Joblin, K. N. and Naylor, G. E. (1993). Inhibition of the rumen anaerobic fungus Neocallimastix frontalis by fermentation products. Lett. Appl. Microbiol ., 16, 254–6.
Joblin, K. N. and Naylor, G. E. (1994). Effects of Butyrivibrio fibrisolvens on gut anaerobic fungi. Proc. Nutr. Soc., 3, 171.
Joblin, K. N. and Naylor, G. E. (1996). Inhibition of Ruminococcus flavefaciens by ruminal fungi. Ann. Zootech, 45 (Suppl. 1), 289.
Joblin, K. N. and Williams, A. G. (1991). Effect of cocultivation of ruminal chytrid fungi with Methanobrevibacter smithii on lucerne stem degradation and extracellular fungal enzyme activities. Lett. Appl. Microbiol., 12, 121–4.
Joblin, K. N., Campbell, G. P., Richardson, A. J. and Stewart, C. S. (1989). Fermentation of barley straw by anaerobic rumen bacteria and fungi in axenic culture and in co-culture with methanogens. Lett. Appl. Microbiol., 9, 195–7.
Joblin, K. N., Naylor, G. E. and Williams, A. G. (1990). The effect of Methanobrevibacter smithii on the xylanolytic activity of anaerobic rumen fungi. Appl. Environ. Microbiol., 56, 2287–95.
Jouany, J. P. (1989). Effects of diet on populations of rumen protozoa in relation to fibre digestion. In The Roles of Protozoa and Fungi in Ruminant Nutrition, ed. J. V. Nolan, R. A. Leng and D. I. Demeyer. Penambul Books, Armidale, pp. 59–94.
Kemp, P., Lander, D. and Orpin, C. G. (1984). The lipids of the anaerobic rumen fungus Piromonas communis. J. Gen. Microbiol., 130, 27–37.
Kemp, P., Jordan, D. J. and Orpin, C. G. (1985). The free and protein amino acids of the rumen phycomycete fungi Neocallimastix frontalis and Piromonas communis. J. Agric. Sci., 105, 523–6.
Kolattukudy, P. E. (1985). Enzymatic penetration of the plant cuticle by fungal pathogens. Annu. Rev. Phytopathol., 23, 223–50.
Kurihara, Y., Eadie, I. M., Hobson, P. N. and Mann, S. O. (1968). Relationship between bacteria and ciliate protozoa in the sheep rumen. J. Gen. Microbiol., 51, 267–88.
Lamed, R., Setter, E., Kenig, R. and Bayer, E. A. (1983). Characterization of a cellulose-binding cellulase-containing complex in Clostridium thermocellum. J. Bacteriol., 156, 828–36.
Leedle, J. A. Z. and Greening, R. C. (1988). Postprandial changes in methanogenic and acidogenic bacteria in the rumen of steers fed high- or low-forage diets once daily. Appl. Environ. Microbiol., 54, 502–6.
Li, X. and Calza, R. E. (1991a). Fractionation of cellulases from the ruminal fungus Neocallimastix frontalis EB 188. Appl. Environ. Microbiol., 57, 3331–6.
Li, X. and Calza, R. E. (1991b). Purification and characterization of an extracellular β-glucosidase from the rumen fungusNeocallimastix frontalis EB188. Enzyme Microbial Technol., 13, 1–7.
Li, J. and Heath, I. B. (1992). The phylogenetic relationships of the anaerobic chytridiomycetous gut fungi (Neocallimasticaceae) and chytridiomycota: 1. Cladistic analysis of rRNA sequences. Can. J. Bot., 70, 1739–46.
Li, J., Heath, I. B., Bauchop, T. (1990). Piromyces mae and Piromyces dumbonica, two new species of monoflagellated anaerobic chytridiomycete fungi from the hindgut of the horse and elephant. Can. J. Bot., 68, 1021–33.
Li, J., Heath, I. B. and Packer, L. (1993). The phylogenetic relationships of the anaerobic chytridiomycete gut fungi (Neocallimasticaceae) and the chytridiomycota: II. Cladistic analysis of structural data and description ofNeocallimasticales ord. nov. Can. J. Bot., 71, 393–7.
Liebetanz, E. (1910). Die parasitischen Protozoen der Wiederkauermagens. Arch. Protist., 19, 19.
Lowe, S. E., Theodorou, M. K., Trinci, A. P. J. and Hespell, R. B. (1985). Growth of anaerobic rumen fungi on defined and semi-defined media lacking rumen fluid. J. Gen. Microbiol., 131, 2225–9.
Lowe, S. E., Griffiths, G. G., Milne, A. et al. (1987a). The life-cycle and growth kinetics of an anaerobic rumen fungus. J. Gen. Microbiol., 133, 1751–8.
Lowe, S. E. Theodorou, M. K. and Trinci, A. P. J. (1987b). Cellulase and xylanase of an anaerobic rumen fungus grown on wheat straw, wheat straw holocellulose, cellulose and xylan. Appl. Environ. Microbiol., 53, 1216–23.
Lowe, S. E., Theodorou, M. K. and Trinci, A. P. J. (1987c). Growth and fermentation of an anaerobic rumen fungus on various carbon sources and effect of temperature on development. Appl. Environ. Microbiol., 53, 1210–15.
Lowe, S. E., Theodorou, M. K. and Trinci, A. P. J. (1987d). Isolation of anaerobic fungi from saliva and faeces of sheep. J. Gen. Microbiol., 133, 1829–934.
Lubinsky, G. (1955a). On some parasites of parasitic protozoa. 1. Sphaerita hoari sp. n. – a chytrid parasitizing Eremoplastron bovis. Can. J. Microbiol., 1, 440–50.
Lubinsky, G. (1955b). On some parasites of parasitic protozoa. II. Sagittospora cameroni gen. n., sp. n. – a phycomycete parasitizing Ophryoscolecidae. Can. J. Microbiol., 1, 675–84.
Lund, A. (1974). Yeasts and moulds in the bovine rumen. J. Gen. Microbiol., 81, 453–62.
Marounek, M. and Hodrova, B. (1989). Susceptibility and resistance of anaerobic rumen fungi to antimicrobial feed additives. Lett. Appl. Microbiol., 9, 173–5.
Marvin-Sikkema, F. D., Richardson, A. J., Stewart, C. S. et al. (1990). Influence of hydrogen-consuming bacteria on cellulose degradation by anaerobic fungi. Appl. Environ. Microbiol., 56, 3793–7.
Marvin-Sikkema, F. D., Pedro-Gomez, T. M., Grivet, J.-P. et al. (1993a). Characterization of the hydrogenosomes and their role in glucose metabolism of Neocallimastix sp. L2. Arch. Mikrobiol., 160, 388–96.
Marvin-Sikkema, F. D., Kraak, M. N., Veenhuis, M. et al. (1993b. The hydrogenosomal enzyme hydrogenase from the anaerobic fungus Neocallimastix sp. L2 is recognised by antibodies directed against the c-terminal microbody protein targeting signal SKL. Eur. J. Cell Biol., 61, 86–91.
McSweeney, C. S., Dulieu, A., Katayama, Y. and Lowry, J. B. (1994). Solubilization of lignin by the ruminal anaerobic fungus Neocallimastix patriciarum. Appl. Environ. Microbiol., 60, 2985–9.
Morgavi, D. P., Sakurada, M., Mizokami, M. et al. (1994). Effects of ruminal protozoa on cellulose degradation and the growth of an anaerobic ruminal fungus, Piromyces sp. strain OTS1, in vitro. Appl. Environ. Microbiol., 60, 3718–23.
Morgavi, D. P., Sakurada, M., Tomita, Y. and Onodera, R. (1994b). Presence in rumen bacterial and protozoal populations of enzymes capable of degrading fungal cell walls. Microbiology, 140, 631–6.
Morvan, B., Bonnemoy, F., Fonty, G. and Gouet, P. (1996). Quantitative determination of H2-utilizing acetogenic and sulphate-reducing bacteria and methanogenic archaea from the digestive tract of different mammals. Curr. Microbiol., 32, 129–33.
Morvan, B., Rieu-Lesme, F., Fonty, G. and Gouet, P. (1996b). In vitro interactions between rumen H2-producing cellulolytic microorganisms and FL-utilising acetogenic and sulphate-reducing bacteria. Anaerobe, 2, 175–80.
Mountfort, D. O. (1994). Regulatory constraints in the degradation and fermentation of carbohydrates by anaerobic fungi. In Anaerobic Fungi: Biology, Ecology and Function, ed. D. O. Mountfort and C. G. Orpin. Marcel Dekker, New York, pp. 147–68.
Mountfort, D. O. and Asher, R. A. (1983). Role of catabolite regulatory mechanisms in control of carbohydrate utilization by the rumen anaerobic fungus Neocallimastix frontalis. Appl. Environ. Microbiol., 46, 1331–8.
Mountfort, D. O. and Asher, R. A. (1985). Production and regulation of cellulase by two strains of the rumen anaerobic fungus Neocallimastix frontalis. Appl. Environ. Microbiol., 49, 1314–22.
Mountfort, D. and Asher, R. A. (1989). Production of xylanase by the ruminal anaerobic fungus Neocallimastix frontalis. Appl. Environ. Microbiol., 55, 1016–22.
Mountfort, D. O. and Orpin, C. G. (eds) (1994). Anaerobic Fungi: Biology, Ecology and Function. Marcel Dekker, New York.
Mountford, D. O., Asher, R. A. and Bauchop, T. (1982). Fermentation of cellulose to methane and carbon dioxide by a rumen anaerobic fungus in a triculture withMethanobrevibacter sp. strain RA1 and Methanosarcina barkeri. Appl. Environ. Microbiol., 44, 128–34.
Munn, E. A. (1994). The ultrastructure of anaerobic fungi. In Anaerobic Fungi: Biology, Ecology and Function, ed. D. E. Mountford and C. G. Orpin. Marcel Dekker, New York, pp. 47–106.
Munn, E. A., Orpin, C. G. and Hall, F. J. (1981). Ultrastructural studies of the free zoospore of the rumen phycomycete Neocallimastix frontalis. J. Gen. Microbiol., 125, 311–23.
Munn, E. A., Orpin, C. G. and Greenwood, C. A. (1987). Organization of the kinetosomes and associated structures of the zoospores of the rumen chytridiomycete Neocallirnastix. Can. J. Bot., 65, 456–65.
Munn, E. A., Orpin, C. G. and Greenwood, C. A. (1988). The relationship of the obligate anaerobic fungi of the rumen. Biosystems, 22, 67–81.
Newbold, C. J. and Hillman, K. (1990). The effect of ciliate protozoa on the turnover of bacterial and fungal protein in the rumen of sheep.Lett. Appl. Microbiol., 11, 100–2.
Obispo, N. E. and Dehority, B. A. (1992). A most probable number method for enumeration of rumen fungi with studies on factors affecting their concentration in the rumen. J. Microbiol. Methods, 16, 259–70.
Odenyo, A. A., Mackie, R. I., Stahl, D. A. and White, B. A. (1994). The use of 16S rRNA-targeted oligonucleotide probes for Ruminococcus species and evidence for bacteriocin production. Appl. Environ. Microbiol., 60, 3688–96.
O’Fallon, J. V., Wright, R. W. and Calza, R. E. (1991). Glucose metabolic pathways in the anaerobic fungus Neocallirnastix frontalis. Biochem. J., 274, 595–9.
Orpin, C. G. (1974). The rumen flagellate Callimastix frontalis: does sequestration occur? J. Gen. Microbiol., 84, 395–8.
Orpin, C. G. (1975). Studies on the rumen flagellate Neocallirnastix frontalis. J. Gen. Microbiol., 91, 249–62.
Orpin, C. G. (1976). Studies on the rumen flagellate Sphaeromonas communis. J. Gen. Microbiol., 94, 270–80.
Orpin, C. G. (1977a). The rumen flagellate Piromonas communis: its life-history and invasion of plant material in the rumen. J. Gen. Microbiol., 99, 107–17.
Orpin, C. G. (1977b). Invasion of plant tissue in the rumen by the flagellate Neocallirnastix frontalis. J. Gen. Microbiol., 98, 423–30.
Orpin, C. G. (1977c). On the induction of zoosporogenesis in the rumen phycomycetes Neocallirnastix frontalis, Piromonas communis and Sphaeromonas communits. J. Gen. Microbiol., 101, 181–9.
Orpin, C. G. (1911d). The occurrence of chitin in the cell walls of the rumen organisms Neocallirnastix frontalis, Piromonas communis and Sphaeromonas communis. J. Gen. Microbiol., 99, 215–18.
Orpin, C. G. (1981). Isolation of cellulolytic phycomycete fungi from the caecum of the horse. J. Gen. Microbiol., 123, 187–96.
Orpin, C. G. (1983/84). The role of ciliate protozoa and fungi in the rumen digestion of plant cell walls. Anim. Fd. Sci. Technol., 10, 121–43.
Orpin, C. G. (1989). Ecology of rumen anaerobic fungi in relation to the nutrition of the host animal. In The Roles of Protozoa and Fungi in Ruminant Digestion, ed. J. V. Nolan, R. A. Leng and D. I. Demeyer. Penambul, Armidale, pp. 29–38.
Orpin, C. G. (1994). Anaerobic fungi: taxonomy, biology and distribution in nature. In Anaerobic Fungi: Biology, Ecology and Function, ed. D. O. Mountford and C. G. Orpin. Marcel Dekker, New York, pp.1–46.
Orpin, C. G. and Bountiff, L. (1978). Zoospore chemotaxis in the rumen phycomycete Neocallirnastix frontalis. J. Gen. Microbiol., 104, 113–22.
Orpin, C. G. and Greenwood, Y. (1986a). Effects of haems and related compounds on growth and zoosporogenesis of the rumen phycomyceteNeocallirnastix frontalisH8. J. Gen. Microbiol., 132, 2179–85.
Orpin, C. G. and Greenwood, Y. (1986b). Nutrition and germination requirements of the rumen phycomycete Neocallirnastix patriciarum. Trans. Br. Mycol. Soc., 86, 103–9.
Orpin, C. G., Greenwood, Y., Hall, F. J. and Paterson, I. (1986). The rumen microbiology of seaweed digestion in Orkney Sheep. J. Appl. Bact., 58, 565–96.
Orpin, C. G. and Joblin, K. N. (1988). Anaerobic fungi. In The Rumen Microbiol Ecosystem, ed. P. N. Hobson, pp. 129–50.
Elsevier Applied Science, London. Orpin, C. G. and Letcher, A. J. (1979). Utilization of cellulose, starch, xylan and other hemicelluloses for growth by the rumen phycomycete Neocallirnastix frontalis. Curr. Microbiol., 3, 121–4.
Orpin, C. G. and Munn, E. A. (1986). Neocallirnastix patriciarum: new member of the Neocallimasticaceae inhabiting the sheep rumen. Trans. Br. Mycol. Soc., 86, 178–81.
Orpin, C. G. and Xue, G. P. (1993). Genetics of fibre degradation in the rumen, particularly in relation to anaerobic fungi, and its modification by recombinant DNA technology. Proceedings of the XVII International Grasslands Congress, 1209–14.
Orpin, C. G., Mathiesen, S. D., Greenwood, Y. and Blix, A. S. (1985). Seasonal changes in the ruminal microflora of the High-Arctic Svalbard reindeer (Rangifer tarandus platyrhynchus. Appl. Environ. Microbiol., 50, 144–51.
Osbourne, J. M. and Dehority, B. A. (1989). Synergism in degradation and utilization of intact forage cellulose, hemicellulose, and pectin by three pure cultures of ruminal bacteria. Appl. Environ. Microbiol., 55, 2247–50.
Pearce, P. D. and Bauchop, T. (1985). Glycosidases of the anaerobic rumen fungus Neocallimastix frontalis grown on cellulosic substrates. Appl. Environ. Microbiol., 49, 1265–9.
Pfyffer, G. E. and Rast, D. M. (1980). The polyol pattern of some fungi not hitherto investigated for sugar alcohols. Exp. My col., 4, 160–70.
Pfyffer, G. E., Boraschi-Gaia, C., Weber, B. et al. 1990). A further report on the occurrence of acyclic alcohols in fungi. My col. Res., 94, 219–22.
Phillips, M. W. and Gordon, G. L. R. (1988). Sugar and polysaccharide fermentation by rumen anaerobic fungi from Australia, Britain and New Zealand. Biosystems, 21, 377–83.
Prins, R. A. and Stewart, C. S. (eds) (1994). Microorganisms in Ruminant Nutrition. Nottingham, Nottingham University Press.
Prins, R. A., Marvin-Sikkema, F. D., van der Geisen, M. and Gottschal, J. C. (1994). Hydrogenosomes of the anaerobic fungus Neocallimastix sp. L2. In Microorganisms in Ruminant Nutrition, ed. R. A. Prins and C. S. Stewart. Nottingham University Press, Nottingham, pp. 179–94.
Rees, E. M. R., Lloyd, D. and Williams, A. G. (1995). The effects of co-cultivation with the acetogen Acetitomaculum ruminis on the fermentative metabolism of the rumen fungi Neocallimastix patriciarum and Neocallimastix strain L2.FEMS Microbiol. Lett., 133, 175–80.
Reymond, P., Durand, R., Hebraud, M. and Febre, M. (1991). Molecular cloning of genes from the rumen anaerobic fungus Neocallimastix frontalis: expression during hydrolase induction. FEMS Microbiol. Lett., 77, 107–12.
Reymond, P., Georjon, C., Roux, B. et al. 1992). Sequence of the phosphoenolpyruvate carboxykinase-encoding cDNA from the rumen fungusNeocallimastix frontalis: comparison of the amino acid sequence with animals and yeast. Gene, 110, 57–63.
Richardson, A. J. and Stewart, C. S. (1990). Hydrogen transfer between Neocallimastix frontalis and Selenomonas ruminantium grown in mixed culture. In Microbiology and Biochemistry of Strict Anaerobes Involved in Interspecies Hydrogen Transfer, ed. J. P. Belaich, M. Bruschi and J. L. Garcia. Plenum Press, New York, pp. 463–6.
Richardson, A. J., Stewart, C. S., Campbell, G. P. et al. 1986). Influence of co-culture with rumen bacteria on the lignocellulolytic activity of phycomycetous fungi from the rumen. Abstracts of XIV International Congress of Microbiology, PG2–24, p. 233.
Rode, L. M., Sharak-Genthner, B. R. and Bryant, M. P. (1981). Syntrophic association of methanol and C02-H2-utilizing species Eubacterium limosum and pectin-fermenting Lachnospira multiparus during growth in a pectin medium. Appl. Environ. Microbiol., 42, 20–2.
Roger, V., Grenet, E., Jamot, J.et al. 1992). Degradation of maize stem by two rumen fungal species, Piromyces communis and Caecomyces communis, in pure cultures or in association with cellulolytic bacteria.Reprod. Nutr. Dev., 32, 321–9.
Roger, Vt, Bernalier, A., Grenet, E. et al. 1993). Degradation of wheat straw and maize stem by a monocentric and a polycentric rumen fungi, alone or in association with rumen cellulolytic bacteria. Anim. Fd. Sci. Technol., 42, 69–82.
Romulo, B., Bird, S. H. and Leng, R. A. (1989). Effects of defaunation and protein supplementation on intake, digestibility, N retention and fungal numbers in sheep fed straw-based diets. In The Roles of Protozoa and Fungi in Ruminant Digestion, ed. J. V. Nolan, R. A. Leng and D. I. Demeyer. Penambul Books, Armidale, Australia, pp. 285–8.
Saul, D. J., Williams, L. C., Grayling, R. A. et al. 1990). CelB, a gene coding for a bifunctional cellulase from the extreme thermophile Caldocellum saccharolyticum. Appl. Environ. Microbiol., 56, 3117–24.
Scheifinger, C. C. and Wolin, M. J. (1973). Propionate formation from cellulose and soluble sugars by combined cultures of Bacteroides succinogenes and Selenomonas ruminantium. Appl. Environ. Microbiol., 26, 789–95.
Sharak-Genthner, B. R. and Bryant, M. P. (1987). Additional characteristics of one-carbon compound utilization by Eubacterium limosum and Acetobacter woodii. Appl. Environ. Microbiol., 53, 471–6.
Sivers, V. S. (1962a). Fungi of the order Mucorales in the rumen of cattle. Mikrobiol. Zh. (CKyyiv), 24, 14–19.
Sivers, V. S. (1962b). Quantity of microscopic fungi in the rumen of cattle during winter and summer. Mikrobiol. Zh. (Kyyiv), 24, 51–8.
Sparrow, F. K. (1960). Aquatic Phy corny cetes, 2nd edn. University of Michigan Press, Ann Arbor.
Stewart, C. S. (1994). Plant-animal and microbial interactions in ruminant fibre degradation. In Microorganisms in Ruminant Nutrition, ed. R. A. Prins and C. S. Stewart. Nottingham University Press, Nottingham, pp. 13–28.
Stewart, C. S. and Richardson, R. J. (1989). Enhanced resistance of anaerobic rumen fungi to the ionophores monensin and lasalocid in the presence of methanogenic bacteria. J. Appl Bacteriol, 66, 85–93.
Stewart, C. S., Duncan, S. H. and Joblin, K. N. (1985). The use of tritiated cellulose for the rapid enumeration of cellulolytic anaerobes. Lett. Appl. Microbiol, 1, 45–99.
Stewart, C. S., Gilmour, J. and McConville, M. L. (1986). Microbial interactions, manipulation and genetic engineering. In New Developments and Future Prospects for Research into Rumen Function, ed. A. Niemann-Sprensen, etc, Brussels. Committee of European Communities Report EUR 10054, pp. 243–57.
Stewart, C. S., Duncan, S. H. and Joblin, K. N. (1987a). Antibiotic manipulation of the rumen microflora: the effects of avoparcin and monensin on the release of tritrium from labelled cellulose by Bacteroides succinogenes and the rumen fungus Neocallimastix frontalis. In Recent Advances in Anaerobic Bacteriology, ed. S. P. Borriello and J. M. Hardie. Martinus Nijhoff, Dordrecht, pp. 108–17.
Stewart, C. S., McPherson, C. A. and Cansunar, E. (1987b). The effect of lasalocid on glucose uptake, hydrogen production and the solubilisation of straw by the anaerobic rumen fungus Neocallimastix frontalis. Lett. Appl. Microbiol, 5, 5–7.
Stewart, C. S., Duncan, S. H., Richardson, A. J. et al.(1992). The inhibition of fungal cellulolysis by cell-free preparations from ruminococci. FEMS Microbiol Lett., 97, 83–8.
Teunissen, M. J., De Kort, G. V. M., Op den Camp, H. J. M. and Huis in’t Veld, J. H. S. (1992). Production of cellulolytic and xylanolytic enzymes during growth of the anaerobic fungus Piromyces sp. on different substrates. J. Gen. Microbiol, 138, 1657–64.
Teunissen, M. J., Hermans, J. M. H., Huis in’t Veld, J. H. S. and Vogels, G. D. (1993). Purification and characterisation of a complex-bound and a free ß-l,4-endoxylanase from the culture fluid of the anaerobic fungus Piromyces sp. Strain E2. Arch. Microbiol, 159, 265–71.
Theodorou, M. K., Gill, M., King-Spooner, C. and Beever, D. E. (1990). Enumeration of anaerobic chytridiomycetes as thallus-forming units: novel method for quantification of fibrolytic fungal populations from the digestive tract ecosystem. Appl Environ. Microbiol, 56, 1073–8.
Theodorou, M. K., Davies, D. R. and Orpin, C. G. (1994). Nutrition and survival of anaerobic fungi. In Anaerobic Fungi: Biology, Ecology and Function, ed. D. O. Mountford and C. G. Orpin. Marcel Dekker, New York, pp. 107–28.
Trinci, A. P. J., Lowe, S. E., Milne, A. and Theodorou, M. K. (1988). Growth and survival of rumen fungi. Biosystems, 21, 357–63.
Ulyatt, M. J., Dellow, D. W., John, A. et al.(1985). The contribution of chewing during eating and rumination to the clearance of digesta from the reticulo-rumen. In Control of Digestion and Metabolism in Ruminants, ed. L. P. Milligan and W. L. Grovum. Prentice-Hall, New Jersey, pp. 498–515.
Ushida, K., Tanaka, H. and Kojima, Y. (1989). A simple in situ method for estimating fungal population size in the rumen. Lett. Appl Mcrobiol, 9, 109–11.
Van der Giezen, M., Marvin-Sikkema, F. D., Gollschal, J. C. and Prins, R. A. (1993). Hydrogenosomes of the anaerobic fungusNeocallimastix sp. L2. In Metabolic Compartmentation in Yeasts, Abstracts of the Sixteenth International Symposium on Yeasts (ISSY16), ed. W. A. Scheffers and J. P. van Dijken. Arnhem, The Netherlands, pp. 304–8.
Vavra, J. and Joyon, L. (1966). Etude sur la morphologie, le cycle évolutif et la position systématique deCallimastix cyclopsis Weissenberg 1912. Prostistologica, 2, 5–16.
Wallace, R. J. and Joblin, K. N. (1985). Proteolytic activity of a rumen anaerobic fungus. FEMS Microbiol Lett., 29, 19–25.
Wallace, R. J. and Munro, C. A. (1986). Influence of the rumen anaerobic fungus Neocallimastix frontalis on the proteolytic activity of a defined mixture of rumen bacteria growing on a solid substrate. Lett. Appl. Microbiol, 3, 23–6.
Warner, A. C. S. (1966). Diurnal changes in the concentrations of microorganisms in the rumens of sheep fed limited diets once daily. J. Gen. Microbiol, 45, 213–35.
Webb, J. and Theodorou, M. K. (1988). A rumen anaerobic fungus of the genus Neocallimastix: ultrastructure of the polyflagellate zoospore and young thallus. Biosystems, 21, 393–401.
Widyastuti, Y., Newbold, C. J., Stewart, C. S. and Orskov, E. R. (1995). Interactions between rumen anaerobic fungi and ciliate protozoa in the degradation of rice straw cell walls. Lett. Appl Microbiol., 20, 61–4.
Williams, A. G. and Coleman, G. S. (1992). The Rumen Protozoa. Springer-Verlag, New York.
Williams, A. G. and Orpin, C. G. (1987a). Polysaccharide degrading enzymes formed by three species of rumen fungi grown on a range of carbohydrate substrates. Can. J. Microbiol, 33, 418–26.
Williams, A. G. and Orpin, C. G. (1987b). Glycoside hydrolase enzymes present in the zoospore and vegetative growth stages of the rumen fungi Neocallimastix patriciarum, Piromonas communis and an unidentified isolate, grown on a range of carbohydrates. Can. J. Microbiol, 33, 427–34.
Williams, A. G. and Withers, S. E. (1991). Effects of ciliate protozoa on the activity of polysaccharide-degrading enzymes and fibre breakdown in the rumen ecosystem. J. Appl Bacteriol., 70, 144–55.
Williams, A. G., Withers, S. E. and Joblin, K. N. (1991). Xylanolysis by cocultures of the rumen fungus Neocallimastix frontalis and ruminai bacteria. Lett. Appl Microbiol, 12, 232–5.
Williams, A. G., Joblin, K. N., Butler, R. D. et al. 1993). Interactions bactéries-protistes dans le rumen. Année Biologique, 32, 13–30.
Williams, A. G., Joblin, K. N. and Fonty, G. (1994). Interactions between the rumen chytrid fungi and other micro-organisms. In The Anaerobic Fungi, ed. C. G. Orpin and D. O. Mountfort. Marcel Dekker, London and New York, pp. 191–227.
Williams, A. G., Withers, S. E., Naylor, G. E. and Joblin, K. N. (1994b). Effect of heterotrophic ruminai bacteria on xylan metabolism by the anaerobic fungus Piromyces communis. Lett. Appl Microbiol, 19, 108–9.
Williams, A. G., Withers, S. E. and Orpin, C. G. (1994). Effect of the carbohydrate growth substrate on polysaccharide enzyme formation by anaerobic fungi isolated from the foregut and hindgut of non-ruminant herbivores and the forestomach of ruminants. Lett. Appl Microbiol, 18, 147–51.
Wilson, C. A. and Wood, T. M. (1992). Studies on the cellulase of the rumen anaerobic fungus Neocallimastix frontalis with special reference to the capacity of the enzyme to degrade crystalline cellulose.Enzyme Microbiol Technol, 14, 258–64.
Windham, W. R. and Akin, D. E. (1984). Rumen fungi and forage fibre degradation. Appl Environ. Microbiol., 48, 473–6.
Wolin, M. J. (1975). Interactions between the bacterial species of the rumen. In Digestion and Metabolism in the Ruminant, ed I. W. McDonald and A. C. I. Warner. University of New England, Sydney, pp. 134–48.
Wood, T. M. and Wilson, C. A. (1995) Studies on the capacity of the cellulase of the anaerobic fungus Piromyces communis P to degrade hydrogen bond ordered cellulose. Appl. Microbiol Biotechnol., 43, 573–8.
Wood, T. M., Wilson, C. A., McCrae, S. I. and Joblin, K. N. (1986). A highly active extracellular cellulase from the anaerobic rumen fungus Neocallimastix frontalis. FEMS Microbiol Lett., 34, 37–10.
Wubah, D. A. and Fuller, M. S. (1991). Studies onCaecomyces communis: morphology and development. Mycologia, 83, 303–10.
Wubah, D. A., Fuller, M. S. and Akin, D. E. (1991). Resistant body formation in Neocallimastix sp., an anaerobic fungus from the rumen of a cow. Mycologia, 83, 40–7.
Xue, G. P., Gobius, K. S. and Orpin, C. G. (1992a). A novel polysaccharide hydrolase cDNA celD from Neocallimastix patriciarum encoding three multifunctional catalytic domains with high endoglucanase, cellobiohydrolase and xylanase activities. J. Gen. Microbiol., 138, 2397–403.
Xue, G. P., Orpin, C. G., Gobius, K. S. et al.(1992b). Cloning and expression of multiple cellulase cDNAs from the anaerobic rumen fungus Neocallimastix patriciarum in Escherichia coli. J. Gen. Microbiol., 138, 1413–20.
Xue, G. P., Gobius, K. S. and Orpin, C. G. (1993). Isolation of a multifunctional cellulase (celE) from the rumen fungus Neocallimastix patriciarum. Proceedings of the XVII International Grasslands Congress, pp. 1221–2.
Yarlett, N. C. (1994). Fermentation product generation in rumen chytridiomyces. In Anaerobic Fungi: Ecology, Biology and Function, ed. D. O. Mountfort and C. G. Orpin. Marcel Dekker, New York, pp. 129–46.
Yarlett, N., Orpin, C. G., Munn, E. A. et al.(1986a). Evidence for hydrogenosomes in the rumen fungus, Neocallimastix patriciarum. Biochem. J., 236, 729–39.
Yarlett, N. C., Yarlett, N., Orpin, C. G. and Lloyd, D. (1986b). Cryopreservation of the anaerobic rumen fungus Neocallimastix patriciarum. Lett. Appl. Microbiol., 3, 1–3.
Zhang, J.-X. and Flint, H. J. (1992). A bifunctional xylanase encoded by thexynAgene of the rumen cellulolytic bacterium Ruminococcus ftavefaciens 17 comprises two dissimilar domains linked by an asparagine/glutamine-rich sequence.Mol. Microbiol., 6, 1013–23.
Zhou, L., Xue, G., Orpin, C. G. et al. 1994). Intronless celB from the aerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase. Biochem. J., 297, 359–64.
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Orpin, C.G., Joblin, K.N. (1997). The rumen anaerobic fungi. In: Hobson, P.N., Stewart, C.S. (eds) The Rumen Microbial Ecosystem. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1453-7_4
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