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Effect of alien ipt gene on hormonal concentrations of plants

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Plant Hormone Signal Perception and Transduction

Abstract

Transgenic plants carrying isopentenyl transferase (ipt) gene and normal tobacco plants (Nicotiana tabacum L.) were analyzed to compare their phytohormone status. Total cytokinin (zeatin, zeatin riboside, isopentenyladenine and isopentenyladenosine) level and free IAA content were always higher in shoots regenerated from transgenic culture although the concentrations were lower in roots. In transgenic plants, IAA-oxidase activity was lower and the concentration of its protectant chlorogenic acid was increased. Transgenic plants also contained lower concentrations of ABA.

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References

  1. Ciha A, Brenner M and Brun W (1977) Rapid separation and quantification of abscisic acid from plant tissues using high performance liquid chromatography. Plant Physiol 59: 821–826

    Article  PubMed  CAS  Google Scholar 

  2. Eder J, Rovenska J, Kutacek M and Cermak V (1988) HPLC analysis of indole compounds in Agrobacterium and transformed tobacco cells. In: Kutacek M, Bandurski R S and Krekule J (eds) Physiology and Biochemistry of Auxins in Plants, pp 389–390

    Google Scholar 

  3. Gamburg KZ (1966) Determination of IAA-oxidase and its inhibiting activity. In: Rakitin YuV (ed) Methods of determination of pipit growth substances and herbicides, pp 57–65. Moscow, Nauka

    Google Scholar 

  4. Kefeli VI (1992) Phytohormones, genome and properties. Ukranian Botanical Journal 49: 81–90

    CAS  Google Scholar 

  5. Kefeli V, Ranaweera K, Piruzyan E, Makarova R, Andnanov V and Yusibov V (1990) Phytohormones in transformed tobacco plants. In: Kutacek M, Elliott M and Machackova I (eds) Hormonal regulation of plant development, pp 137–144. The Hague, Academic Publishers

    Google Scholar 

  6. Kulaeva ON (1991) Cytokinin action on transcription and translation in plants. In: Metabolism and molecular activities of cy tokinins, pp 218–227. Berlin, Springer-Verlag

    Google Scholar 

  7. Marineau B, Houck M, Sheehy R and Hiatt W (1994) Fruit-specific expression of the A. tumefaciens isopentenyl tranferase gene in tomato: effects on fruit ripening and defense-related gene expression in leaves. Plant J 5: 11–19

    Article  Google Scholar 

  8. Montague M J, Enns R, Siegel N and Jaworski, E (1981) Inhibition of 2,4-dichlorphenoxyacetic acid conjugation to amino acids by treatment of cultured soybean cells with cytokinins. Plant physiol 67: 701–704

    Article  PubMed  CAS  Google Scholar 

  9. Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plantarum 15: 473–497

    Article  CAS  Google Scholar 

  10. Petersen S, Stummann B, Olsen P and Henningsen K (1989) Structure and function of root-inducing (Ri) plasmids and their relation to tumor inducing (Ti) plasmids. Physiol Plantarum 77: 427–437

    Article  CAS  Google Scholar 

  11. Schell J (1987) Transgenic plants as tools to study the molecular organization of plant genes. Science 237: 1176–1182

    Article  Google Scholar 

  12. Machackova I, Krekule J, Eder J, Seidlova F and Stmad M (1993) Cytokinins in photoperiodic induction of flowering in Chenopodium species. Physiol Plantarum 57: 160–166

    Article  Google Scholar 

  13. Vahala T, Eriksson T, Tillberg E and Nicander B (1993) Expression of a cytokinin synthesis gene from A. tumefaciens T-DNA in basket willow (Salix viminalis). Physiol Plantarum 88:439–445

    Article  CAS  Google Scholar 

  14. Yusibov V, Pak Chun II, Andnanov V and Piruzian E (1991) Phenotypically normal transgenic T-cyt tobacco plants as a model for the investigation of plant expression in response to phytohormonal stress. Plant Mol Biol 17: 825–836

    Article  PubMed  CAS  Google Scholar 

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© 1996 Kluwer Academic Publishers

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Makarova, R.V., Borisova, T.A., Machackova, I., Kefeli, V.I. (1996). Effect of alien ipt gene on hormonal concentrations of plants. In: Smith, A.R., et al. Plant Hormone Signal Perception and Transduction. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0131-5_23

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  • DOI: https://doi.org/10.1007/978-94-009-0131-5_23

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6546-7

  • Online ISBN: 978-94-009-0131-5

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