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Production of Monoclonal Antibodies in Glycoengineered Pichia pastoris

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Antibody Expression and Production

Part of the book series: Cell Engineering ((CEEN,volume 7))

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Abstract

Although improvements in antibody expression by mammalian cells are nearing maturation, efforts to improve antibody efficacy through glycoengineering are rapidly expanding. For example, the production of full length monoclonal antibodies with uniform human N-linked glycans by glycoengineered yeast has been used to optimize antibody effector function. The glycoengineered yeast expression platform not only enables elucidation of structure function relationships but also offers a robust and economically viable alternative to mammalian cell expression. This chapter provides an overview of glycobiology, engineering of P. pastoris to secrete recombinant proteins with uniform human N-linked glycans as well as bioprocess considerations in the production of full length monoclonal antibodies by a yeast based expression system.

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Authors and Affiliations

  1. GlycoFi Inc. (A wholly-owned subsidiary of Merck & Co Inc.), Lebanon, NH, 03766, USA

    Dongxing Zha

  2. Bioprocess Development, Merck & Co, Rahway, NJ, 07065, USA

    Thomas Linden & Thomas Potgieter

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  1. Dongxing Zha
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  2. Thomas Linden
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Correspondence to Dongxing Zha .

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  1. Dept. Chemical & Biochemical, Engineering, University College, Dublin, Belfield, Dublin 4, Ireland

    Mohamed Al-Rubeai

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Zha, D., Linden, T., Potgieter, T. (2011). Production of Monoclonal Antibodies in Glycoengineered Pichia pastoris . In: Al-Rubeai, M. (eds) Antibody Expression and Production. Cell Engineering, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1257-7_4

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