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Microscopic Measurements of the Chlorophyll a Fluorescence Kinetics

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Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications

Part of the book series: Developments in Applied Phycology ((DAPH,volume 4))

Abstract

The method of high resolution fluorescence kinetics combines classical chlorophyll variable fluorescence methods and microscopy. It is useful for studies of intra-population relationships among photosynthetic microorganism, studies of single algal cell metabolism and phytoplankton diversity. The technique can be also used for toxicity tests and to assess the physiological homogeneity of algal populations. On the other hand, the technique is highly time consuming and suffers from relatively low signal and high noise. Recently, combination of multi-spectral methods and fast kinetic measurements with 2D imaging increased potential to study metabolic dynamics of single cells and intracellular compounds. New methods that were developed to manipulate with the living cells are also mentioned.

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Abbreviations

AL:

actinic light

CCD:

charge coupled device

chla :

chlorophyll a

2D, 3D:

two, three dimensional

F′:

fluorescence signal at any point between Fo′ and Fm′

FK:

fluorescence kinetics

FKM:

fluorescence kinetic microscope

FKMI:

fluorescence kinetic microscopy imaging

Fm, Fm′:

maximal fluorescence of dark adapter and light adapted sample

Fo, Fo′:

dark adapted, light adapted minimal fluorescence

FR:

far red illumination

Fv:

variable fluorescence of light adapted state

LED:

light emitting diodes

ML:

measuring light

NPQ:

non photochemical quenching

OJIP:

fast part of Kautsky kinetic curve

PAM:

pulse amplitude modulation

PQ:

plastoquinon

Q −A :

completely oxidized quinon

SP:

saturating pulse (radiation)

UV:

ultraviolet radiation

VIS:

visual radiation (400–700 nm)

IR:

infrared radiation

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Acknowledgments

Financial support was provided by EEA Fund. No A/CZ0046/1/0021 and by the project GACR GA (206/07/0917).

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Authors and Affiliations

  1. Laboratory of Photosynthesis, Institute of Microbiology ASCR, Opatovický Mlýn, 379 01, Třeboň, Czech Republic

    Ondrej Komárek, Kristina Felcmanová, Eva Šetlíková, Eva Kotabová & Ondrej Prášil

  2. Photon Systems Instruments Ltd, Koláčkova 39, Řečkovice, Brno, 621 00, Czech Republic

    Martin Trtílek

Authors
  1. Ondrej Komárek
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  2. Kristina Felcmanová
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  3. Eva Šetlíková
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  4. Eva Kotabová
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  5. Martin Trtílek
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  6. Ondrej Prášil
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Corresponding author

Correspondence to Ondrej Komárek .

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Editors and Affiliations

  1. , Department of Biological Sciences, University of Essex, Colchester, CO4 3SQ, United Kingdom

    David J. Suggett

  2. Institute of Microbiology, Opatovciky mlyn, Trebon, 379 81, Czech Republic

    Ondrej Prášil

  3. Murdoch University, School of Biological Sciences and Biotec, Algae R&D Center, Murdoch, 6150, West Australia, Australia

    Michael A. Borowitzka

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Komárek, O., Felcmanová, K., Šetlíková, E., Kotabová, E., Trtílek, M., Prášil, O. (2010). Microscopic Measurements of the Chlorophyll a Fluorescence Kinetics. In: Suggett, D., Prášil, O., Borowitzka, M. (eds) Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications. Developments in Applied Phycology, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9268-7_5

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