Abstract
Xanthomonas translucens pv. graminis(Xtg) causes bacterial wilt in many forage grasses including Italian ryegrass (Lolium multiflorum Lam), seriously reducing yield and quality. Breeding for resistance is currently the only practicable means of disease control. Molecular markers closely linked to resistance genes or QTL could complement and support phenotypic selection. We used comparative gene expression analysis of a partially resistant L. multiflorum genotype infected and not infected with Xtg to identify genes involved in the control of resistance to bacterial wilt. The genes differentially expressed upon infection will serve as the basis for the identification of key genes involved in bacterial wilt resistance and to develop molecular markers for marker assisted breeding. Fluorescently labelled cDNA prepared from plant leaves collected at four different time points after infection was hybridized to a cDNA microarray containing 10,000 unique genes from L. perenne. Comparisons and statistical analyses of the gene expression profiles revealed 0, 20, 52 and 124 differentially regulated genes 8, 48, 192 and 288 h after infection compared to non-infected controls and considering a p-value threshold of 0.01. This is the first genome-wide transcriptome analysis of L. multiflorum investigating the reaction to Xtg infection.
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Acknowledgements
This research has been financed by Swiss National Science Foundation SNF Project (3100A0-112582). We would like to thank L. B. Jensen for technical assistance in the lab of DJF, H.-P. Piepho and A. Schützenmeister for help with the experimental design and Hubert Rehrauer from the Functional Genomics Center Zürich for assistance with statistical analyses.
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Wichmann, F., Asp, T., Widmer, F., Kölliker, R. (2010). Identification of Genes Induced in Lolium multiflorum by Bacterial Wilt Infection. In: Huyghe, C. (eds) Sustainable use of Genetic Diversity in Forage and Turf Breeding. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-8706-5_72
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DOI: https://doi.org/10.1007/978-90-481-8706-5_72
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