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Plant Biology and Biotechnology pp 205–221Cite as

DNA Fingerprinting Techniques for Plant Identification

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Abstract

Advances in knowledge about the molecular structure of plant genome and development of techniques to rapidly characterise genomic variations have established DNA fingerprinting as a powerful tool for identifying plant species, varieties, clones, individuals and even plant products. This article provides details of DNA polymorphism in plants and classical and more recent molecular marker techniques based on restriction fragment length polymorphism (RFLP), polymerase chain reaction (PCR), DNA microarrays and sequencing. The advantages and limitations of each technique are given along with some recent examples of their application in plant identification.

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  1. Asia-Pacific Association of Agricultural Research Institutions, National Agricultural Science Complex, Pusa, New Delhi, 110 012, India

    J. L. Karihaloo

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  1. Sri Biotech Laboratories India Limited, Hyderabad, Telangana, India

    Bir Bahadur

  2. Department of Genetics, University of Delhi, New Delhi, Delhi, India

    Manchikatla Venkat Rajam

  3. Division of Biotechnology, Indian Institute of Horticultural Research (IIHR), Bangalore, Karnataka, India

    Leela Sahijram

  4. Center for Pharmaceutics, Pharmacognosy and Pharmacology, School of Life Sciences, Institute of Trans-Disciplinary Health Science and Technology (IHST), Bangalore, Karnataka, India

    K. V. Krishnamurthy

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Karihaloo, J.L. (2015). DNA Fingerprinting Techniques for Plant Identification. In: Bahadur, B., Venkat Rajam, M., Sahijram, L., Krishnamurthy, K. (eds) Plant Biology and Biotechnology. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2283-5_9

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