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Azospirillum: A Biofertilizer for Every Crop

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Book cover Plant Microbes Symbiosis: Applied Facets

Abstract

Azospirillum is known for its nitrogen-fixing and phytohormone production ability. It is one of very well-studied plant growth-promoting rhizobacteria, at lab scale to field. None of its species or strain is reported as human or plant pathogen. It is considered as safest bacteria which can be used as a biofertilizer at commercial level for several crops, especially cereals or grasses including wheat and rice which are of economic importance for the whole world. Some of its species are reported for phosphate-solubilizing ability and high salt tolerance. Fifteen of its species have been isolated from variety of hosts and environmental sources; however, a majority have been reported from plants. There are several reviews available on this organism; in this chapter, an overview of this organism covering its plant growth-promoting abilities, used as inoculum in lab and field experiments and used as a commercial biofertilizer for different crops, is provided.

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References

  • Alen’kina SA, Payusova OA, Nikitina VE (2006) Effect of Azospirillum lectins on the activities of wheat-root hydrolytic enzymes. Plant Soil 283:147–151

    Google Scholar 

  • Ayrault G (2002) Seed germinability and plant establishment of Lactuca sativa and Daucus carota inoculated with Azospirillum and exposed to salt stress. MSc dissertation, University of Mar del Plata, Argentina, p 90

    Google Scholar 

  • Babu RS, Sankaranarayanan C, Jothi G (1998) Management of Pratylenchus zeae on maize by biofertilizers and VAM. Ind J Nematol 28:77–80

    Google Scholar 

  • Bacilio M, Vazquez P, Bashan Y (2003) Alleviation of noxious effects of cattle ranch composts on wheat seed germination by inoculation with Azospirillum spp. Biol Fertil Soils 38:261–266

    Google Scholar 

  • Bacilio M, Rodriguez H, Moreno M, Hernandez JP, Bashan Y (2004) Mitigation of salt stress in wheat seedlings by a gfp-tagged Azospirillum lipoferum. Biol Fertil Soils 40:188–193

    CAS  Google Scholar 

  • Bansal RK, Dahiya RS, Lakshminarayana K, Suneja S, Anand RC, Narula N (1999) Effect of rhizospheric bacteria on plant growth of wheat infected with Heterodera avenae. Nematol Mediterr 27:311–314

    Google Scholar 

  • Barassi CA, Ayrault G, Creus CM, Sueldo RJ, Sobrero MT (2006) Seed inoculation with Azospirillum mitigates NaCl effects on lettuce. Sci Hortic 109:8–14

    CAS  Google Scholar 

  • Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. Crit Rev Plant Sci 24:23–58

    CAS  Google Scholar 

  • Bashan Y (1990) Short exposure to Azospirillum brasilense Cd inoculation enhanced proton efflux in intact wheat roots. Can J Microbiol 36:419–425

    CAS  Google Scholar 

  • Bashan Y (1991) Changes in membrane potential of intact soybean root elongation zone cells induced by Azospirillum brasilense. Can J Microbiol 37:958–963

    Google Scholar 

  • Bashan Y, de-Bashan LE (2002) Protection of tomato seedlings against infection by Pseudomonas syringae pv. tomato by using the plant growth-promoting bacterium Azospirillum brasilense. Appl Environ Microbiol 68:2637–2643

    PubMed  CAS  PubMed Central  Google Scholar 

  • Bashan Y, de-Bashan LE (2010) How the plant growth promoting bacterium Azospirillum promotes plant growth – a critical assessment. Adv Agron 108:77–136

    CAS  Google Scholar 

  • Bashan Y, Levanony H (1990) Current status of Azospirillum inoculation technology: Azospirillum as a challenge for agriculture. Can J Microbiol 36:591–608

    CAS  Google Scholar 

  • Bashan Y, Levanony H (1991) Alterations in membrane potential and in proton efflux in plant roots induced by Azospirillum brasilense. Plant Soil 137:99–103

    Google Scholar 

  • Bashan Y, Alcaraz-Melendez L, Toledo G (1992) Responses of soybean and cowpea root membranes to inoculation with Azospirillum brasilense. Symbiosis 13:217–228

    Google Scholar 

  • Bashan Y, Holguin G, de-Bashan LE (2004) Azospirillum-plant relationships: physiological, molecular, agricultural and environmental advances (1997–2003). Can J Microbiol 50:521–577

    PubMed  CAS  Google Scholar 

  • Bashan Y, Bustillos JJ, Leyva LA, Hernandez JP, Bacilio M (2006) Increase in auxiliary photoprotective photosynthetic pigments in wheat seedlings induced by Azospirillum brasilense. Biol Fertil Soils 42:279–285

    CAS  Google Scholar 

  • Belimov A, Dietz KJ (2000) Effect of associative bacteria on element composition of barley seedlings grown in solution culture at toxic cadmium concentrations. Microbiol Res 155:113–121

    PubMed  CAS  Google Scholar 

  • Belimov AA, Kunakova AM, Safronova VI, Stepanok VV, Yudkin LY, Alekseev YV, Kozhemyakov AP (2004) Employment of rhizobacteria for the inoculation of barley plants cultivated in soil contaminated with lead and cadmium. Microbiology (Moscow) 73:99–106

    CAS  Google Scholar 

  • Bottini R, Cassan F, Piccoli P (2004) Gibberellin production by bacteria and its involvement in plant growth promotion and yield increase. Appl Microbiol Biotechnol 65:497–503

    PubMed  CAS  Google Scholar 

  • Bouillant ML, Miché L, Ouedraogo O, Alexandre G, Jacoud C, Sallé G, Bally R (1997) Inhibition of Striga seed germination associated with sorghum growth promotion by soil bacteria. C R Acad Sci Paris-Sciences de la vie 320:159–162

    Google Scholar 

  • Boyer M, Bally R, Perrotto S, Chaintreuil C, Wisniewski-Dye F (2008) A quorum quenching approach to identify quorum-sensing regulated functions in Azospirillum lipoferum. Res Microbiol 20:72–77

    Google Scholar 

  • Brasil MS, Baldani VLD, Baldani JI, Souto SM (2005) Effects of inoculation of diazotrophs in grasses Pantanal. Pasturas Tropicales 27:22–33

    Google Scholar 

  • Cacciari I, Lippi D, Pietrosanti T, Pietrosanti W (1989) Phytohormone-like substances produced by single and mixed diazotrophic cultures of Azospirillum spp. and Arthrobacter. Plant Soil 115:151–153

    CAS  Google Scholar 

  • Carrillo AE, Li CY, Bashan Y (2002) Increased acidification in the rhizosphere of cactus seedlings induced by Azospirillum brasilense. Naturwissenschaften 89:428–432

    PubMed  CAS  Google Scholar 

  • Carrozzi LE (2005) Lactuca sativa (L.) seed priming and Azospirillum inoculations a tool for improving germination rate. MSc dissertation, University of Mar del Plata, Argentina

    Google Scholar 

  • Casanovas EM, Barassi CA, Sueldo RJ (2002) Azospirillum inoculation mitigates water stress effects in maize seedlings. Cer Res Commun 30:343–350

    Google Scholar 

  • Cassan F, Maiale S, Masciarelli O, Vidal A, Luna V, Ruiz O (2009a) Cadaverine production by Azospirillum brasilense and its possible role in plant growth promotion and osmotic stress mitigation. Eur J Soil Biol 45:12–19

    CAS  Google Scholar 

  • Cassan F, Perrig D, Sgroy V, Masciarelli O, Penna C, Luna V (2009b) Azospirillum brasilense Az39 and Bradyrhizobium japonicum E109, inoculated singly or in combination, promote seed germination and early seedling growth in corn (Zea mays L.) and soybean (Glycine max L.). Eur J Soil Biol 45:28–35

    CAS  Google Scholar 

  • Chang TT, Li CY (1998) Weathering of limestone, marble, and calcium phosphate by ecto-mycorrhizal fungal and associated microorganisms. Taiwan J Sci 13:8590

    Google Scholar 

  • Cohen AC, Bottini R, Piccoli PN (2008) Azospirillum brasilense Sp 245 produces ABA in chemically-defined culture medium and increases ABA content in Arabidopsis plants. Plant Growth Regul 54:97–103

    CAS  Google Scholar 

  • Cohen AC, Travaglia CN, Bottini R, Piccoli PN (2009) Participation of abscisic acid and gibberellins produced by endophytic Azospirillum in the alleviation of drought effects in maize. Botany 87:455–462

    CAS  Google Scholar 

  • Correa-Aragunde N, Graziano M, Lamattina L (2004) Nitric oxide plays a central role in determining lateral root development in tomato. Planta 218:900–905

    PubMed  CAS  Google Scholar 

  • Costacurta A, Vanderleyden J (1995) Synthesis of phytohormones by plant-associated bacteria. Crit Rev Microbiol 21:1–18

    PubMed  Google Scholar 

  • Costacurta A, Keijers V, Vanderleyden J (1994) Molecular cloning and sequence analysis of an Azospirillum brasilense indole-3-pyruvate decarboxylase gene. Mol Gen Genet 243:463–472

    PubMed  CAS  Google Scholar 

  • Creus CM, Sueldo RJ, Barssi CA (1997) Shoot growth and water status in Azospirillum inoculated wheat seedlings grown under osmotic and salt stresses. Plant Physiol Biochem 35:939–944

    CAS  Google Scholar 

  • Creus CM, Suelda RJ, Barassi CA (2004) Water relations and yield in Azospirillum-inoculated wheat exposed to drought in the field. Can J Bot 82:273–281

    Google Scholar 

  • Creus CM, Graziano M, Casanovas EM, Pereyra MA, Simontacchi M, Puntarulo S, Barassi CA, Lamattina L (2005) Nitric oxide is involved in the Azospirillum brasilense-induced lateral root formation in tomato. Planta 221:297–303

    PubMed  CAS  Google Scholar 

  • Crozier A, Arruda P, Jasmin JM, Monteiro AM, Sandberg G (1988) Analysis of indole-3-acetic acid and related indoles in culture medium from Azospirillum lipoferum and Azospirillum brasilense. Appl Environ Microbiol 54:2833–2837

    PubMed  CAS  PubMed Central  Google Scholar 

  • Dadon T, Bar Nun N, Mayer AM (2004) A factor from Azospirillum brasilense inhibits germination and radicle growth of Orobanche aegyptiaca. Isr J Plant Sci 52:83–86

    CAS  Google Scholar 

  • Dalla Santa OR, Hernandez RF, Alvarez GLM, Ronzelli JP, Soccol CR (2004) Azospirillum sp. inoculation in wheat, barley and oats seeds greenhouse experiments. Braz Arch Biol Tech 47:843–850

    Google Scholar 

  • de-Bashan LE, Bashan Y (2008) Joint immobilization of plant growth-promoting bacteria and green microalgae in alginate beads as an experimental model for studying plant–bacterium interactions. Appl Environ Microbiol 74:6797–6802

    PubMed  CAS  PubMed Central  Google Scholar 

  • de-Bashan LE, Antoon H, Bashan Y (2005) Cultivation factors and population size control uptake of nitrogen by the microalgae Chlorella vulgaris when interacting with the microalgae growth-promoting bacterium Azospirillum brasilense. FEMS Microbiol Ecol 54:197–203

    PubMed  CAS  Google Scholar 

  • Deubel A, Gransee A, Merbach W (2000) Transformation of organic rhizodepositions by rhizosphere bacteria and its influence on the availability of tertiary calcium phosphate. J Plant Nutr Soil Sci 163:387–392

    CAS  Google Scholar 

  • Diaz-Zorita M, Fernandez-Canigia MV (2009) Field performance of a liquid formulation of Azospirillum brasilense on dryland wheat productivity. Eur J Soil Biol 45:3–11

    Google Scholar 

  • Didonet AD, Didonet CCGM, Gomes GF (2003) Evaluation of strains of upland rice inoculated with Azospirillum lipoferum Sp59b and A. brasilense Sp24. Comunicado Tecnico EMBRAPA, p 69

    Google Scholar 

  • Dobbelaere S, Croonenborghs A, Thys A, Vande Broek A, Vanderleyden J (1999) Phytostimulatory effect of Azospirillum brasilense wild type and mutant strains altered in IAA production on wheat. Plant Soil 212:155–164

    CAS  Google Scholar 

  • El-Khawas H, Adachi K (1999) Identification and quantification of auxins in culture media of Azospirillum and Klebsiella and their effect on rice roots. Biol Fertil Soils 28:377–381

    CAS  Google Scholar 

  • El-Komy HM, Hamdia MA, El-Baki GKA (2003) Nitrate reductase in wheat plants grown under water stress and inoculated with Azospirillum spp. Biol Plant 46:281–287

    CAS  Google Scholar 

  • Fallik E, Okon Y, Epstein E, Goldman A, Fischer M (1989) Identification and quantification of IAA and IBA in Azospirillum brasilense inoculated maize roots. Soil Biol Biochem 21:147–153

    CAS  Google Scholar 

  • Ferreira MCB, Fernandes MS, Dobereiner J (1987) Role of Azospirillum brasilense nitrate reductase in nitrate assimilation by wheat plants. Biol Fertil Soils 4:47–53

    CAS  Google Scholar 

  • Garcia de Salamone IE, Dobereiner J, Urquiaga S, Boddey RM (1997) Biological nitrogen fixation in Azospirillum strain–maize genotype associations as evaluated by the 15N isotope dilution technique. Biol Fertil Soils 23:249–256

    Google Scholar 

  • Giraud E, Moulin L, Vallenet D, Barbe V, Cytryn E, Avarre JC, Jaubert M, Simon D, Cartieaux F, Prin Y, Bena G, Hannibal L, Fardoux J, Kojadinovic M, Vuillet L, Lajus A, Cruveiller S, Rouy Z, Mangenot S, Segurens B, Dossat C, Franck WL, Chang WS, Saunders E, Bruce D, Richardson P, Normand P, Dreyfus B, Pignol D, Stacey G, Emerich D, Vermeglio A, Medigue C, Sadowsky M (2007) Legumes symbioses: absence of genes in nod in photosynthetic bradyrhizobia. Science 316:1307–1312

    PubMed  Google Scholar 

  • Glick BR, Patten CL, Holguin G, Penrose DM (1999) Biochemical and genetic mechanisms used by plant growth promoting bacteria. Imperial College Press, London, pp 125–140

    Google Scholar 

  • Goncalves AFS, de Oliveira RGB (1998) Cyanide production by Brazilian strains of Azospirillum. Rev Microbiol 29:36–39

    CAS  Google Scholar 

  • Hamaoui B, Abbadi JM, Burdman S, Rashid A, Sarig S, Okon Y (2001) Effects of inoculation with Azospirillum brasilense on chickpeas (Cicer arietinum) and faba beans (Vicia faba) under different growth conditions. Agronomie 21:553–560

    Google Scholar 

  • Hartmann A, Zimmer W (1994) Physiology of Azospirillum. In: Okon Y (ed) Azospirillum/plant association. CRC Press, Boca Raton, pp 15–39

    Google Scholar 

  • Hartmann A, Singh M, Klingmuller W (1983) Isolation and characterization of Azospirillum mutants excreting high amounts of indole acetic acid. Can J Microbiol 29:916–923

    CAS  Google Scholar 

  • Hassouna MG, El-Saedy MAM, Saleh HMA (1998) Biocontrol of soil-borne plant pathogens attacking cucumber (Cucumis sativus) by rhizobacteria in a semiarid environment. Arid Soil Res Rehabil 12:345–357

    CAS  Google Scholar 

  • Hernandez JP, de-Bashan LE, Bashan Y (2006) Starvation enhances phosphorus removal from wastewater by the microalga Chlorella spp. co-immobilized with Azospirillum brasilense. Enzyme Microb Technol 38:190–198

    CAS  Google Scholar 

  • Holguin G, Bashan Y (1996) Nitrogen-fixing by Azospirillum brasilense Cd is promoted when co-cultured with a mangrove rhizosphere bacterium (Staphylococcus sp.). Soil Biol Biochem 28:1651–1660

    CAS  Google Scholar 

  • Horemans S, De Koninck K, Neuray J, Hermans R, Vlassak K (1986) Production of plant growth substances by Azospirillum sp. and other rhizosphere bacteria. Symbiosis 2:341–346

    CAS  Google Scholar 

  • Kaneko T, Minamisawa K, Isawa T, Nakatsukasa H, Mitsui H, Kawaharada Y, Nakamura Y, Watanabe A, Kawashima K, Ono A, Shimizu Y, Takahashi C, Minami C, Fujishiro T, Kohara M, Katoh M, Nakazaki N, Nakayama S, Yamada M, Tabata S, Sato S (2010) Complete genomic structure of the cultivated rice endophyte Azospirillum sp. B510. DNA Res 17(1):37–50

    PubMed  CAS  PubMed Central  Google Scholar 

  • Kasim WA, Osman ME, Omar MN, Abd-Eldeim IA, Bejai S, Meijer J (2013) Control of drought stress in wheat using plant growth promoting bacteria. J Plant Growth Regul 32:122–130

    CAS  Google Scholar 

  • Kavitha K, Meenakumari KS, Sivaprasad P (2003) Effect of dual inoculation of native arbuscular mycorrhizal fungi and Azospirillum on suppression of damping off in chilli. Ind Phytopathol 56:112–113

    Google Scholar 

  • Kennedy IR, Choudhry ATMA, Kecskes ML (2004) Non-symbiotic bacterial diazotrophs in crop-farming systems: can their potential for plant growth promotion be better explored. Soil Biol Biochem 36:1229–1244

    CAS  Google Scholar 

  • Khan MR, Kounsar K (2000) Effect of seed treatment with certain bacteria and fungi on the growth of mung bean and reproduction of Meloidogyne incognita. Nematol Mediterr 28:221–226

    Google Scholar 

  • Kishore P (1998) Response of sorghum variety Pusa chari-121 to carrier based inoculants (Azotobacter and Azospirillum), fermented residue and shootfly (Atherigona soccata Rondani) under field conditions. J Entomol Res 22:101–105

    Google Scholar 

  • Kyungseok P, Kloepper JW, Ryu CM (2008) Rhizobacterial exopolysaccharides elicit induced resistance on cucumber. J Microbiol Biotechnol 18:1095–1100

    Google Scholar 

  • Lavrinenko K, Chernousova E, Gridneva E, Dubinina G, Akimov V, Kuever J, Lysenko A, Grabovich M (2010) Azospirillum thiophilum sp. nov., a novel diazotrophic bacterium isolated from a sulfide spring. Int J Syst Evol Microbiol 60:2832–2837

    PubMed  CAS  Google Scholar 

  • Lin SY, Young CC, Hupfer H, Siering C, arun AB, Chen WM, Lai WA, Shen FT, Rekha PD, Yasin AF (2009) Azospirillum picis sp. nov., isolated from discarded tar. Int J Syst Evol Microbiol 59:761–765

    PubMed  CAS  Google Scholar 

  • Lombardo MC, Graziano M, Polacco JC, Lamattina L (2006) Nitric oxide functions as a positive regulator of root hair development. Plant Signal Behav 1:28–33

    PubMed  PubMed Central  Google Scholar 

  • Lucangeli C, Bottini R (1997) Effects of Azospirillum spp. on endogenous gibberellin content and growth of maize (Zea mays L.) treated with uniconazole. Symbiosis 23:63–72

    CAS  Google Scholar 

  • Lucy M, Reed E, Glick BR (2004) Application of free living plant growth promoting rhizobacteria. Antonie van Leeuwenhoek Int J Gen Mol Biol 86:1–25

    CAS  Google Scholar 

  • Lyubun YV, Fritzsche A, Chernyshova MP, Dudel EG, Fedorov EE (2006) Arsenic transformation by Azospirillum brasilense Sp245 in association with wheat (Triticum aestivum L.) roots. Plant Soil 286:219–227

    CAS  Google Scholar 

  • Malhotra M, Srivastava S (2006) Targeted engineering of Azospirillum brasilense SM with indole acetamide pathway for indole acetic acid over-expression. Can J Microbiol 52:1078–1084

    PubMed  CAS  Google Scholar 

  • Malhotra M, Srivastava S (2008) An ipdc gene knock out of Azospirillum brasilense strain SM and its implications on indole 3-acetic acid biosynthesis and plant growth promotion. Antonie van Leeuwenhoek J Gen 93:425–433

    CAS  Google Scholar 

  • Malhotra M, Srivastava S (2009) Stress responsive indole-3-acetic acid biosynthesis by Azospirillum brasilense SM and its ability to modulate plant growth. Eur J Soil Biol 45:73–80

    CAS  Google Scholar 

  • Manivannan M, Tholkappian P (2013) Prevalence of Azospirillum isolates in tomato rhizosphere soils of coastal areas of Cuddalore District, Tamil Nadu. Int J Recent Sci Res 4:1610–1613

    Google Scholar 

  • Mehnaz S, Lazarovits G (2006) Inoculation effects of Pseudomonas putida, Gluconacetobacter azotocaptans and Azospirillum lipoferum on corn plant growth under green house conditions. Microb Ecol 51:326–335

    PubMed  Google Scholar 

  • Mehnaz S, Weselowski B, Lazarovits G (2007a) Azospirillum canadense sp. nov., a nitrogen fixing bacterium isolated from corn rhizosphere. Int J Syst Evol Microbiol 57(3):620–624

    PubMed  CAS  Google Scholar 

  • Mehnaz S, Weselowski B, Lazarovits G (2007b) Azospirillum zeae sp. nov., diazotrophic bacteria isolated from rhizosphere soil of Zea mays. Int J Syst Evol Microbiol 57(12):2805–2809

    PubMed  CAS  Google Scholar 

  • Miché L, Bouillant ML, Rohr R, Sallé G, Bally R (2000) Physiological and cytological studies on the inhibition of Striga seed germination by the plant growth-promoting bacterium Azospirillum brasilense. Eur J Plant Pathol 106:347–351

    Google Scholar 

  • Molina-Favero C, Creus CM, Lanteri ML, Correa-Aragunde N, Lombardo MC, Barassi CA, Lamattina L (2007) Nitric oxide and plant growth promoting rhizobacteria: common features influencing root growth and development. Adv Bot Res 46:1–33

    Google Scholar 

  • Molina-Favero C, Creus CM, Simontacchi M, Puntarulo S, Lamattina L (2008) Aerobic nitric oxide production by Azospirillum brasilense Sp245 and its influence on root architecture in tomato. Mol Plant Microbe Interact 21:1001–1009

    PubMed  CAS  Google Scholar 

  • Molla AH, Shamsuddin ZH, Saud HM (2001) Mechanism of root growth and promotion of nodulation in vegetable soybean by Azospirillum brasilense. Commun Soil Sci Plant Anal 32:2177–2187

    CAS  Google Scholar 

  • Moutia YJF, Saumtally S, Spaepen S, Vanderleyden J (2010) Plant growth promotion by Azospirillum sp. in sugarcane is influenced by genotype and drought stress. Plant Soil 337:233–242

    CAS  Google Scholar 

  • Nikitina VE, Bogomolova NV, Ponomareva EG, Sokolov OI (2004) Effect of azospirilla lectins on germination capacity of seeds. Biol Bull (Moscow) 31:354–357

    Google Scholar 

  • Okon Y (1985) Azospirillum as a potential inoculants for agriculture. Trends Biotechnol 3:223–228

    Google Scholar 

  • Okon Y, Vanderleyden J (1997) Root-associated Azospirillum species can stimulate plants. Appl Environ Microbiol 63:366–370

    Google Scholar 

  • Okumura RS, Mariano DC, Dallacort R, Nogueira de Albuquerque A, Lobato AKS, Guedes EMS, Neto CFO, Oliveira da Conceicao HE, Alves GAR (2013) Azospirillum: a new and efficient alternative to biological nitrogen fixation in grasses. J Food Agric Environ 2(1):1142–1146

    Google Scholar 

  • Oliveira RGB, Drozdowicz A (1987) Inhibition of bacteriocin producing strains of Azospirillum lipoferum by their own bacteriocin. Zentralblatt fur Mikrobiologie 142:387–391

    Google Scholar 

  • Ona O, Smets I, Gysegom P, Bernaerts K, Impe JV, Prinsen E, Vanderleyden J (2003) The effect of pH on indole-3-acetic acid biosynthesis of Azospirillum brasilense Sp7. Symbiosis 35:199–208

    CAS  Google Scholar 

  • Pagnussat GC, Simontacchi M, Puntarulo S, Lamattina L (2002) Nitric oxide is required for root organogenesis. Plant Physiol 129:954–956

    PubMed  CAS  PubMed Central  Google Scholar 

  • Pagnussat GC, Lanteri ML, Lamattina L (2003) Nitric oxide and cyclic GMP are messengers in the indole acetic acid-induced adventitious rooting process. Plant Physiol 132:1241–1248

    PubMed  CAS  PubMed Central  Google Scholar 

  • Pedraza RO, Motok J, Tortora ML, Salazar SM, Dı’az Ricci JC (2007) Natural occurrence of Azospirillum brasilense in strawberry plants. Plant Soil 295:169–178

    CAS  Google Scholar 

  • Pereyra MA, Zalazar CA, Barassi CA (2006) Root phospholipids in Azospirillum inoculated wheat seedlings exposed to water stress. Plant Physiol Biochem 44:873–879

    PubMed  CAS  Google Scholar 

  • Perrig D, Boiero ML, Masciarelli OA, Penna C, Ruiz OA, Cassan FD, Luna MV (2007) Plant-growth-promoting compounds produced by two agronomically important strains of Azospirillum brasilense, and implications for inoculant formulation. Appl Microbiol Biotechnol 75:1143–1150

    PubMed  CAS  Google Scholar 

  • Piccoli P, Masciarelli O, Bottini R (1999) Gibberellin production by Azospirillum lipoferum cultured in chemically defined medium as affected by oxygen availability and water status. Symbiosis 27:135–145

    CAS  Google Scholar 

  • Prigent-Combaret C, Blaha D, Pothier JF, Vial L, Poirier MA, Wisniewski-Dyé F, Moe¨nne-Loccoz Y (2008) Physical organization and phylogenetic analysis of acdR as leucine-responsive regulator of the 1-aminocyclopropane-1-carboxylate deaminase gene acdS in phytobeneficial Azospirillum lipoferum 4B and other proteobacteria. FEMS Microbiol Ecol 65:202–219

    PubMed  CAS  Google Scholar 

  • Puente ME, Bashan Y, Li CY, Lebsky VK (2004) Microbial populations and activities in the rhizoplane of rock-weathering desert plants I. Root colonization and weathering of igneous rocks. Plant Biol 6:629–642

    PubMed  CAS  Google Scholar 

  • Ramakrishnan S, Gunasekaran CR, Vadivelu S (1997) Effect of bio-fertilizers Azolla and Azospirillum on root-knot nematode, Meloidogyne incognita and plant growth of okra. Ind J Nematol 26:127–130

    Google Scholar 

  • Reis VM, Teixeira KRS, Pedraza RO (2011) What is expected from the genus Azospirillum as a plant growth promoting bacteria? In: Maheshwari DK (ed) Bacteria in agrobiology: plant growth responses. Springer, Berlin/Heidelberg, pp 123–138

    Google Scholar 

  • Remans R, Beebe S, Blair M, Manrique G, Tovar E, Rao I, Croonenborghs A, Torres-Gutierrez R, El-Howeity M, Michiels J, Vanderleyden J (2008) Physiological and genetic analysis of root responsiveness to auxin-producing plant growth-promoting bacteria in common bean (Phaseolus vulgaris L.). Plant Soil 302:149–161

    CAS  Google Scholar 

  • Reynders L, Vlassak K (1979) Conversion of tryptophan to indole acetic acid by Azospirillum brasilense. Soil Biol Biochem 11:547–548

    CAS  Google Scholar 

  • Rodrigues EP, Rodrigues LS, de Oliveira ALM, Baldani VLD, Teixeira KRD, Urquiaga S, Reis VM (2008) Azospirillum amazonense inoculation: effects on growth, yield and N2-fixation of rice (Oryza sativa L.). Plant Soil 302:249–261

    CAS  Google Scholar 

  • Rodriguez H, Fraga R (1999) Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17:319–339

    PubMed  CAS  Google Scholar 

  • Rodriguez H, Gonzalez T, Goire I, Bashan Y (2004) Gluconic acid production and phosphate solubilization by the plant growth-promoting bacterium Azospirillum spp. Naturwissenschaften 91:552–555

    PubMed  CAS  Google Scholar 

  • Rodriguez-Salazar J, Suarez R, Caballero-Mellado J, Iturriaga G (2009) Trehalose accumulation in Azospirillum brasilense improves drought tolerance and biomass in maize plants. FEMS Microbiol Lett 296:52–59

    PubMed  CAS  Google Scholar 

  • Rueda-Puente EO, Castellanos T, Troyo-Dieguez E, Diaz de Leon-Avarez JL (2004) Effect of Klebsiella pneumonia and Azospirillum halopraeferens on the growth and development of two Salicornia bigelovii genotypes. Aust J Exp Agric 44:65–74

    Google Scholar 

  • Saikia SP, Jain V, Khetarpal S, Aravind S (2007) Dinitrogen fixation activity of Azospirillum brasilense in maize (Zea mays). Curr Sci 93:1296–1300

    CAS  Google Scholar 

  • Sala VMR, Freitas SS, Donzeli VP, Freitas JG, Gallo PB, Silveira APD (2005) Occurrence and effect of diazotrophic bacteria in wheat genotypes. Revista Brasileira de Ciencia do Solo 29:345–352

    Google Scholar 

  • Sala VMR, Cardoso EJBN, Freitas JG, Silveira APD (2007) Wheat genotypes response to inoculation of diazotrophic bacteria in field conditions. Pesq Agrop Brasileira 42:833–842

    Google Scholar 

  • Sankari JU, Dinakar S, Sekar C (2011) Dual effect of Azospirillum exo-polysaccharides (EPS) on the enhancement of plant growth and biocontrol of blast (Pyricularia oryzae) disease in upland rice (var. ASD-19). J Phytol 3(10):16–19

    Google Scholar 

  • Sant’Anna FH, Almeida LGP, Cecagno R, Reolon LA, Siqueira FM, Machado MRS, Vasconcelos ATR, Schrank IS (2011) Genomics insight into the versatility of the plant growth promoting bacterium Azospirillum amazonense. BMC Genomics 12:409

    PubMed  PubMed Central  Google Scholar 

  • Sarig S, Okon Y, Blum A (1990) Promotion of leaf area development and yield in Sorghum bicolor inoculated with Azospirillum brasilense. Symbiosis 9:235–245

    Google Scholar 

  • Saubidet MI, Barneix AJ (1998) Growth stimulation and nitrogen supply to wheat plants inoculated with Azospirillum brasilense. J Plant Nutr 21:2565–2577

    CAS  Google Scholar 

  • Saubidet MI, Fatta N, Barneix AJ (2002) The effect of inoculation with Azospirillum brasilense on growth and nitrogen utilization by wheat plants. Plant Soil 245:215–222

    CAS  Google Scholar 

  • Seshadri S, Muthukumuramasamy R, Lakshiminarasami C, Ignacimuthu S (2000) Solubilization of inorganic phosphates by Azospirillum halopraeferans. Curr Sci 79:565–567

    CAS  Google Scholar 

  • Sgroy V, Cassan F, Masciarelli O, Del Papa MF, Lagares A, Luna V (2009) Isolation and characterization of endophytic plant growth-promoting (PGPB) or stress homeostasis-regulating (PSHB) bacteria associated to the halophyte Prosopis strombulifera. Appl Microbiol Biotechnol 85:371–381

    PubMed  CAS  Google Scholar 

  • Shah S, Karkhanis V, Desai A (1992) Isolation and characterization of siderophore, with antimicrobial activity, from Azospirillum lipoferum. Curr Microbiol 25:347–351

    CAS  Google Scholar 

  • Somers E, Ptacek D, Gysegom P, Srinivasan M, Vanderleyden J (2005) Azospirillum brasilense produces the auxin like phenylacetic acid by using the key enzyme for indole 3-acetic acid biosynthesis. Appl Environ Microbiol 71:1803–1810

    PubMed  CAS  PubMed Central  Google Scholar 

  • Spaepen S, Vanderleyden J, Remans R (2007) Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS Microbiol Rev 31:425–448

    PubMed  CAS  Google Scholar 

  • Spaepen S, Vanderleyden J, Okon Y (2009) Plant growth promoting actions of rhizobacteria. Adv Bot Res 51:283–320

    CAS  Google Scholar 

  • Steenhoudt O, Vanderleyden J (2000) Azospirillum, a free living nitrogen fixing bacterium closely associated with grasses: genetic, biochemistry and ecological aspects. FEMS Microbiol Rev 24:487–506

    PubMed  CAS  Google Scholar 

  • Strzelczyk E, Kampert M, Li CY (1994) Cytokinin-like substances and ethylene production by Azospirillum in media with different carbon sources. Microbiol Res 149:55–60

    CAS  Google Scholar 

  • Sudhakar P, Chattopadhyay GN, Gangwar SK, Ghosh JK (2000) Effect of foliar application of Azotobacter, Azospirillum and Beijerinckia on leaf yield and quality of mulberry (Morus alba). J Agric Sci 134:227–234

    Google Scholar 

  • Tahir M, Mirza MS, Zaheer A, Dimitrov MR, Smidt H, Hameed S (2013) Isolation and identification of phosphate solubilizer Azospirillum, Bacillus and Enterobacter strain by 16S rRNA sequence analysis and their effect on growth of wheat (Triticum aestivum L.). Aust J Crop Sci 7(9):1284–1292

    CAS  Google Scholar 

  • Tapia-Hernandez A, Mascarua-Esparza M, Caballero-Mellado J (1990) Production of bacteriocins and siderophore-like activity by Azospirillum brasilense. Microbios 64:73–83

    PubMed  CAS  Google Scholar 

  • Tarrand JJ, Kreig NR, Dobereiner J (1978) A taxonomic study of the Spirillum lipoferum group with description of a new genus Azospirillum gen.nov., and two species, Azospirillum lipoferum (Beijerinck) com nov. and Azospirillum brasilense sp. nov. Can J Microbiol 24:967–980

    PubMed  CAS  Google Scholar 

  • Thuler DS, Floh EIS, Handro W, Barbosa HR (2003) Plant growth regulators and amino acids released by Azospirillum sp in chemically defined media. Lett Appl Microbiol 37:174–178

    PubMed  CAS  Google Scholar 

  • Tien TM, Gaskins MH, Hubell DH (1979) Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Pennisetum americanum L). Appl Environ Microbiol 37:1016–1024

    PubMed  CAS  PubMed Central  Google Scholar 

  • Tortora ML, Diaz-Ricci JC, Pedraza RO (2011) Azospirillum brasilense siderophores with antifungal activity against Colletotrichum acutatum. Arch Microbiol 193:275–286

    PubMed  CAS  Google Scholar 

  • Walker V, Bertrand C, Bellvert F, Moenne-Loccoz Y, Bally R (2011) Host plant secondary metabolite profiling shows a complex strain dependent response of maize to plant growth promoting rhizobacteria of the genus Azospirillum. New Phytol 189:494–506

    PubMed  CAS  Google Scholar 

  • Wisniewski-Dye F, Lozano L, Acosta-Cruz E, Borland S, Drogue B, Prigent-Combaret C, Rouy Z, Barbe V, Herrera AM, Gonzalez B, Mavingui P (2012) Genome sequence of Azospirillum brasilense CBG497 and comparative analysis of Azospirillum core and accessory genomes provide insight into niche adaptation. Genes 3:576–602

    PubMed  CAS  PubMed Central  Google Scholar 

  • Yahalom E, Okon Y, Dovrat A (1990) Possible mode of action of Azospirillum brasilense strain Cd on the root morphology and nodule formation in burr medic (Medicago polymorpha). Can J Microbiol 36:10–14

    Google Scholar 

  • Yasuda M, Isawa T, Shinozaki S, Minamisawa K, Nakashita H (2009) Effects of colonization of a bacterial endophyte, Azospirillum sp. B510, on disease resistance in rice. Biosci Biotechnol Biochem 73:2595–2599

    PubMed  CAS  Google Scholar 

  • Zakharova E, Shcherbakov A, Brudnik V, Skripko N, Bulkhin N, Ignatov V (1999) Biosynthesis of indole-3-acetic acid in Azospirillum brasilense. Insights from quantum chemistry. Eur J Biochem 259:572–576

    PubMed  CAS  Google Scholar 

  • Zakharova EA, Iosipenko AD, Ignatov VV (2000) Effect of water soluble vitamins on the production of indole-3-acetic acid by Azospirillum brasilense. Microbial Res 155:209–214

    CAS  Google Scholar 

  • Zhou Y, Wei W, Wang X, Xu L, Lai R (2009) Azospirillum palatum sp. nov. isolated from forest soil in Zhejiang province, China. J Gen Appl Microbiol 55:1–7

    PubMed  CAS  Google Scholar 

  • Zimmer W, Roeben K, Bothe H (1988) An alternative explanation for plant growth promotion by bacteria of the genus Azospirillum. Planta 176:333–342

    PubMed  CAS  Google Scholar 

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  1. Department of Biological Sciences, Forman Christian College (A Chartered University), Ferozepur Road, Lahore, 54600, Pakistan

    Samina Mehnaz

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  1. Department of Environmental Microbiology, Babasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh, India

    Naveen Kumar Arora

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Mehnaz, S. (2015). Azospirillum: A Biofertilizer for Every Crop. In: Arora, N. (eds) Plant Microbes Symbiosis: Applied Facets. Springer, New Delhi. https://doi.org/10.1007/978-81-322-2068-8_15

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