Abstract
Natural virus inhibitors from higher plants are known to inhibit the infection of several plant viruses in hypersensitive and systemic test hosts. During the present investigation, the clarified sap from dried leaves of the medicinal plant Operculina turpethum L. (Family: Convolvulaceae) was sprayed on the leaves of several economically important crop plants, viz. Lycopersicon esculentum, Lagenaria siceraria (Mol). Standl., Crotalaria juncea L. and Solanum tuberosum L. in microplots under field conditions which prevented the infection and multiplication of tomato yellow mottle mosaic virus (TmYMV), cucumber green mottle mosaic virus (CGMMV), sunn-hemp rosette virus (SRV) and potato virus-x (PVX). Almost complete protection was observed and treated plants showed no disease symptoms if six sprays were given prior to virus challenge and two postinoculation sprays were given at weekly intervals.
The O. turpethum-clarified sap was completely safe and non-phytotoxic. The treated plants were healthy and showed no systemic symptoms of virus infection.
The resistant leaves’ sap extracted from treated crop plants and assayed under in vitro conditions with their respective viruses on their hypersensitive hosts showed the ability to inhibit virus infectivity. The induction of systemic antiviral state in treated plants presumably involved the production of some highly active induced virus-interfering agent(s), such agent(s) were absent in nontreated control crop plants which showed severe disease symptoms.
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Acknowledgments
Dr. M. M. Abid Ali Khan is highly grateful to the Director, CIMAP-CSIR, Lucknow for appointment as a Research Associate (CSIR) during the tenure of the present investigations in this research paper.
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Khan, M., Zaidi, S., Kazmi, S., Musanna, S. (2014). Prevention of Virus Infection and Multiplication by Inducing Virus-Interfering Agent(s) in Treated Crop Plants Under Field Conditions. In: Kharwar, R., Upadhyay, R., Dubey, N., Raghuwanshi, R. (eds) Microbial Diversity and Biotechnology in Food Security. Springer, New Delhi. https://doi.org/10.1007/978-81-322-1801-2_18
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DOI: https://doi.org/10.1007/978-81-322-1801-2_18
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