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Archetypical and Specialized DNA Replication Proteins in Entamoeba histolytica

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Amebiasis

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Abstract

Accurate DNA replication and repair are essential tasks for survival. In eukaryotes, DNA polymerases replicate genomes that can be composed of billions of base pairs. These genomes can be chemically damaged or modified, jeopardizing its integrity, and cells have evolved mechanisms to ameliorate the mutagenic effect of DNA damage. DNA replication and DNA lesion bypass in bacteria, yeast, and humans have been widely studied; however, little is known about these processes in other organisms. Entamoeba histolytica is a parasitic protozoan responsible for amebic dysentery and hepatic abscess. Herein, we define the DNA replication apparatus of Entamoeba histolytica and review the biochemical peculiarities of family A and family B2 DNA polymerases involved in DNA lesion bypass. Our data indicate that E. histolytica is a mosaic of archetypical family B DNA polymerases (α, ε, and δ) present at the replication fork and specialized DNA polymerases with novel lesion bypass properties.

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Acknowledgments

Research in the LGB laboratory is supported by CONACYT-grant 128647. We thank the Howard Hughes Medical Institute for support.

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Authors and Affiliations

  1. Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Km. 9.6 Libramiento Norte Carretera Irapuato-León 36821, Irapuato Gto. México, CP 36500, Irapuato, Guanajuato, Mexico

    Guillermo Pastor-Palacios, Varinia López-Ramírez, Cesar S. Cardona-Félix & Luis G. Brieba

  2. Universidad Autónoma de la Ciudad de México, Posgrado en Ciencias Genómicas, San Lorenzo 290, CP 03100, México DF, Mexico

    Elisa Azuara Liceaga

  3. División de Biología Molecular, IPICYT, Camino a la Presa, San José 2055, CP 78216, San Luis Potosí, Mexico

    Samuel Lara-Gonzalez

Authors
  1. Guillermo Pastor-Palacios
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  2. Varinia López-Ramírez
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  3. Cesar S. Cardona-Félix
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  4. Elisa Azuara Liceaga
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  5. Samuel Lara-Gonzalez
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  6. Luis G. Brieba
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Corresponding author

Correspondence to Luis G. Brieba .

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Editors and Affiliations

  1. Department of Parasitology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan

    Tomoyoshi Nozaki

  2. Jawaharlal Nehru University, New Delhi, India

    Alok Bhattacharya

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Pastor-Palacios, G., López-Ramírez, V., Cardona-Félix, C.S., Liceaga, E.A., Lara-Gonzalez, S., Brieba, L.G. (2015). Archetypical and Specialized DNA Replication Proteins in Entamoeba histolytica . In: Nozaki, T., Bhattacharya, A. (eds) Amebiasis. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55200-0_22

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