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CRISPR-Cas Systems pp 145–169Cite as

Distribution and Mechanism of the Type I CRISPR-Cas Systems

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Abstract

Although the CRISPR type I system encompasses six different subtypes (I-A to I-F), only three subtypes have been studied in detail to date. This review includes an analysis of the distribution of CRISPR-Cas systems among the different bacterial and archaeal lineages, and will focus on our mechanistic understanding of the type I-E system of Escherichia coli. We will cover the overall organization of this system, starting with a detailed description of a typical type I-E gene cluster and its associated CRISPR array. In addition, we will describe recent insights on the three different stages in CRISPR-Cas type I-mediated defense: adaptation, expression and crRNA maturation, and interference. A comparison will be presented of the physical and functional characteristics of CRISPR effector complexes from the various subtypes.

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Authors and Affiliations

  1. Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB, Wageningen, The Netherlands

    Raymond H. J. Staals & Stan J. J. Brouns

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  1. Raymond H. J. Staals
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  2. Stan J. J. Brouns
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Correspondence to Stan J. J. Brouns .

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Editors and Affiliations

  1. DuPont Nutrition and Health, Agriculture Drive 3329, Madison, 53716, Wisconsin, USA

    Rodolphe Barrangou

  2. , Laboratory of Microbiology, University of Wageningen, Dreijenplein 10, Wageningen, 6703 HB, Netherlands

    John van der Oost

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Staals, R.H.J., Brouns, S.J.J. (2013). Distribution and Mechanism of the Type I CRISPR-Cas Systems. In: Barrangou, R., van der Oost, J. (eds) CRISPR-Cas Systems. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-34657-6_6

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