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Nucleic Acid Extraction, Purification, Reannealing, and Hybridization Methods

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Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

The study of nucleic acids is an important area of research in biology and particularly in genetics. DNA is “the molecule of heredity”, and RNA is the keystone in transferring genetic information into cellular structure and function. The techniques developed to study nucleic acids are providing tools to understanding such questions as the organization of the eukaryotic genome, species taxonomic relationships, chromosome structure, and potential productivity. This chapter describes certain nucleic acid techniques that have been applied to forest genetics research.

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References

  • Berlyn, G.P., Miksche, J.P.: Botanical microtechnique and cytochemistry. Ames, Iowa: Iowa State Univ. Press 1976.

    Google Scholar 

  • Bernardi, G.: Chromatography of nucleic acids on hydroxyapatite columns. In: Methods in Enzymology, Vol. 21 (eds. Grossmann and Moldave), pp. 95–139. New York: Academic Press 1971.

    Google Scholar 

  • Bolton, E.T., McCarthy, B.J.: A general method for the isolation of RNA complementary to DNA. Proc. Nat’l Acad. Sci. (USA) 48, 1390–1397 (1962).

    Article  CAS  Google Scholar 

  • Britten, R.J., Kohne, D.E.: Repeated sequences in DNA. Science 161, 529–540 (1968).

    Article  PubMed  CAS  Google Scholar 

  • Comings, D.E., Riggs, A.D.: Molecular mechanism of chromosome pairing, folding and function. Nature 233, 48–50 (1971).

    Article  PubMed  CAS  Google Scholar 

  • Crothers, D.M.: On the mechanism of deoxyribonucleic acid unwinding. Accts Chem. Res. 2, 225–232 (1969).

    Article  CAS  Google Scholar 

  • Denhardt, D.T.: A membrane filter technique for the detection of complementary DNA. Biochem. Biophys. Res. Comm. 23, 641–646 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Dingman, C.W., Kakefuda, T., Fisher, M.P.: Electrophoretic properties of low molecular weight DNA fragments in agarose-acrylamide gels. Anal. Biochem. 50, 519–528 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Gillespie, D., Spiegelman, S.: A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. Mol. Biol. 12, 829–842 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Hall, B.D., Spiegelman, S.: Sequence complementarity of T2-DNA and T2 specific RNA. Proc. Nat’l. Acad. Sci. (USA) 47, 137–146 (1961).

    Article  CAS  Google Scholar 

  • Hall, R.B.: Variation in DNA content per cell and repetitious DNA of selected populations of Pinus banksiana Lamb. Ph.D. Thesis University of Wisconsin, Madison, 209 pp. (1974).

    Google Scholar 

  • Hall, R.B., Stairs, G.R., Miksche, J.P.: Variation in DNA content and redundancy - possible significance to forest tree breeding. Proc. 8th Cent. State Forest Tree Improv. Conf., School of Forestry, Fisheries and Wildlife. Columbia, Missouri: Univ. of Missouri 1974.

    Google Scholar 

  • Hayashi, M., Hayashi, M.N., Spiegelman, S.: Chromatographic separation of annealed and enzymatically synthesized RNA-DNA hybrids. Biophys. J. 5 (2), 231–246 (1965).

    Article  PubMed  Google Scholar 

  • Honda, S.I., Hongladarom, T., Laties, G.G.: A new isolation medium for plant organelles. J. Exp. Botany 17, 460–472 (1966).

    Article  CAS  Google Scholar 

  • Hotta, Y., Miksche, J.P.: Molecular hybridization of coniferous ribosomal RNA to DNA. Proc. 8th Cent. State Forest Tree Improv. Conf., School of Forestry, Fisheries and Wildlife. Columbia, Missouri: Univ. of Missouri 1974.

    Google Scholar 

  • Jaworski, A., Key, J.L.: Distribution of ribosomal deoxyribonucleic acid in subcellular fractions of higher plants. Plant Physiol. 53, 366–369 (1974).

    Article  PubMed  CAS  Google Scholar 

  • Kennel, D.E.: Principles and practices of nucleic acid hybridization. In: Progress in Nucleic Acid Research and Molecular Biology (eds. J.N. Davidson and W.E. Cohn ). New York: Academic Press 1971.

    Google Scholar 

  • King, R.C.: The meiotic behavior of the Drosophila oocyte. Intern. Rev. Cytology 28, 125–168 (1970).

    Article  CAS  Google Scholar 

  • Kirk, J.T.O., Reese, H., Evans, F.: Base composition of nuclear DNA within the genus Allium. Heredity 25, 507–512 (1971).

    Article  Google Scholar 

  • Mandel, M., Marmur, J.: Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. In: Methods in Enzymology (eds. L. Grossman and K. Moldave), XII Part B, pp. 195–206. New York: Academic Press 1968.

    Google Scholar 

  • Mandel, M., Schildkraut, C.L., Marmur, J.: Use of CsCl density gradient analysis for determining the guanine plus cytosine content of DNA. In: Methods in Enzymology (eds. Grossman and Moldave), XII Part B, pp. 184–194. New York: Academic Press 1968.

    Google Scholar 

  • Marmur, J., Rowand, R., Schildkraut, C.L.: Denaturation and renaturation of deoxyribonucleic acid. Prog. Nucleic Acid Research 1, 231–300 (1963).

    Article  CAS  Google Scholar 

  • Miksche, J.P., Hotta, Y.: DNA base compositions and repetitious DNA in several conifers. Chromosoma 41, 29–36 (1973).

    Article  CAS  Google Scholar 

  • Miksche, J.P., Hotta, Y.: Preliminary evaluation of biochemical hybridization methods. 3rd North Amer. For. Biology Workshop 361 (eds. C.P.P. Reid and G.H. Fechner ). Fort Collins, Colorado: Colorado State Univ. 1974.

    Google Scholar 

  • Nygaard, A.P., Hall, B.D.: A method for the detection of RNA-DNA complexes. Biochem. Biophys. Res. Comm. 12, 98–104 (1963).

    Article  PubMed  CAS  Google Scholar 

  • Peacock, A.C., Dingman, C.W.: Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry 7, 668–674 (1968).

    Article  PubMed  CAS  Google Scholar 

  • Pearson, G.G., Ingle, J.: The origin of stress induced satellite DNA in plant tissues. Cell Differentiation 1, 43–52 (1972).

    Article  CAS  Google Scholar 

  • Rehfeldt, G.E., Lester, D.T.: Specialization and flexibility in genetic systems of forest trees. Silvae Genetica 18, 118–123 (1969).

    Google Scholar 

  • Spencer, D., Wildman, S.G.: The incorporation of amino acids into protein by cell-free extracts from toba leaves. Biochemistry 3, 954–959 (1964).

    Article  PubMed  CAS  Google Scholar 

  • Stern, H.: Isolation and purification of plant nucleic acids from whole tissues and from isolated nuclei. In: Methods in Enzymology (eds. L. Grossman and K. Moldave), XII Part B, pp. 100–112. New York: Academic Press 1968.

    Google Scholar 

  • Studier, F.W.: Sedimentation studies of the size and shape of DNA. J. Mol. Biol. 11, 373–390 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Sutton, W.D.: Chromatin packing, repeated DNA sequences and gene control. Nature New Biology 237, 70–71 (1972).

    PubMed  CAS  Google Scholar 

  • Szybalski, W.: Equilibrium sedimentation of viruses, nucleic acids, and other macromolecules in density gradients, pp. 1–15. Palo Alto, Calif.: Beckman Instruments 1968.

    Google Scholar 

  • Tewari, K.K., Wildman, S.G.: Chloroplast DNA from tobacco leaves. Science 153, 1269–1271 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Walker, P.M.B.: The specificity of molecular hybridization in relation to studies on higher organisms. Prog. Nucl. Acid Res. and Mol. Biol. 9, 301–326 (1969).

    Article  CAS  Google Scholar 

  • Warnaar, S.O., Cohen, J.A.: A quantitative assay for DNA-DNA hybrids using membrane filters. Biochem. Biophys. Res. Comm. 24, 554–558 (1966).

    Article  CAS  Google Scholar 

  • Whitefield, P.R., Spencer, D.: Buoyant density of tobacco and spinach chloroplast DNA. Biochim. et Biophys. Acta 157, 333–343 (1968).

    Google Scholar 

  • Wimber, D.E., Steffensen, D.M.: Localization of 5S RNA genes on drosophila chromosomes by RNA-DNA hybridization. Science 170, 639–641 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Wimber, D.E., Steffensen, D.M.: Localization of gene function. Annual Review of Genetics 7, 205–223 (1974).

    Article  Google Scholar 

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© 1976 Springer-Verlag Berlin Heidelberg

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Hall, R.B., Miksche, J.P., Hansen, K.M. (1976). Nucleic Acid Extraction, Purification, Reannealing, and Hybridization Methods. In: Miksche, J.P. (eds) Modern Methods in Forest Genetics. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-12523-6_2

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  • DOI: https://doi.org/10.1007/978-3-662-12523-6_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-12525-0

  • Online ISBN: 978-3-662-12523-6

  • eBook Packages: Springer Book Archive

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