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Dionaea muscipula Ellis (Venus Flytrap): In Vitro Cultures and in Vitro Production of Secondary Metabolites

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Medicinal and Aromatic Plants XII

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 51))

Abstract

In 1768, William Young, the royal botanist, imported living plants of the Venus fly-trap to England. They were shown to John Ellis, a member of the Royal Society, who recognised the Venus as a carnivorous plant. He wrote a letter and sent it with a dried plant to the Swedish scientist, Carl von Linné. Among others Ellis wrote: “Nature may have some views towards its nourishment in forming the upper joint of its leaf like a machine to catch food: upon the middle of this lies the bait for the unhappy insect that becomes its prey . .. the two lobes rise up, grasp it fast, lock the rows of spines together, and squeeze it to death ... the small erect spines are fixed near the middle of each lobe, over the glands, that effectually put an end to all its struggles”. Linné gave this species the name Dionaea muscipula Ellis (Slack 1985; Juniper et al. 1989). This name comes from the Greek word Dionaia, the goddess of love.

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References

  • Ahn D, Putt D, Kresty L, Stoner GD, Fromm D, Hollenberg PF (1996) The effects of dietary ellagic acid on rat hepatic and esophageal mucosal cytochrome P450 and phase II enzymes. Carcinogenesis 17:821–828

    Article  PubMed  CAS  Google Scholar 

  • Bhattacharayya J, Kokpol U, Miles DH (1976) The isolation from Sarracenia flava and partial synthesis of betulinaldehyde. Phytochemistry 15:432–433

    Article  Google Scholar 

  • Beebe JD (1980) Morphogenetic responses of seedlings and adventitious buds of the carnivorous plant Dionaea muscipula in aseptic culture. Bot Gaz 141:396–400

    Article  Google Scholar 

  • Budzianowski J (1995) Naphthoquinones of Drosera spathulata from in vitro cultures. Phytochemistry 40:1145–1148

    Article  CAS  Google Scholar 

  • Budzianowski J (1996) Naphthohydroquinone glucosides of Drosera rotundifolia and D. intermedia from in vitro cultures. Phytochemistry 42:1145–1147

    Article  CAS  Google Scholar 

  • Budzianowski J(1997) 2-Methyl naphthazarin 5-O-glucoside from the methanolic extracts of in vitro cultures of Drosera species. Phytochemistry 44:75–77

    Article  CAS  Google Scholar 

  • Budzianowski J (2000) Naphthoquinone glucosides of Drosera gigantea from in vitro cultures. Planta Med 66:667–669

    Article  PubMed  CAS  Google Scholar 

  • Budzianowski J, Pakulski G, Skrzypczak L (1993a) Ellagic acid derivatives and further naphthoquinones from Dionaea muscipula and four species of the genus Drosera from in vitro cultures. 41th Annu Congr Med Plant Res, 31 August-4 September 1993, Düsseldorf, Abstr, p 79. Planta Med 59 (Suppl A 654)

    Google Scholar 

  • Budzianowski J, Skrzypczak L, Kukulczanka K (1993b) Phenolic compounds of Drosera intermedia and D. spathulata from in vitro cultures. Acta Hortic 330:277–280

    Google Scholar 

  • Budzianowski J, Pakulski G, Skrzypczak L (1995) Phenolic metabolites of Droseraceae species from in vitro cultures. 43th Annu Congr Med Plant Res, Halle (Saale), Abstr, p 34

    Google Scholar 

  • Budzianowski J, Ratajczak L, Skrzypczak L (1996) Flavonoids of Droseraceae species from in vitro cultures. 44th Annu Congr Med Plant Res, Prague, Abstr, p 140

    Google Scholar 

  • Budzianowski J, Ratajczak L, Kromer K (1998) Phenolic compounds in further species of the genus Drosera L. from in vitro cultures. 46th Annu Congr Med Plant Res, 30 August-4 September 1998, Vienna, Abstr, p C19

    Google Scholar 

  • Crouch IJ, Finnie JF, van Staden J (1990) Studies on the isolation of plumbagin from in vitro and in vivo grown Drosera species. Plant Cell Tissue Organ Cult 21:79–82

    Article  CAS  Google Scholar 

  • Culham A, Gornall RJ (1994) The taxonomic significance of naphthoquinones in the Droseraceae. Biochem Syst Ecol 22:507–515

    Article  CAS  Google Scholar 

  • Di Gregorio GJ, Di Palma JR (1966) Anthocyanin in Dionaea muscipula Ellis (Venus’s flytrap) Nature (Lond) 212:1264–1265

    Article  Google Scholar 

  • Dietzel U, Sauer R, Reichardt U (1985) Erfahrungen mit Carnivora. Fortschr Med 103:760–781

    PubMed  CAS  Google Scholar 

  • Drees M, Berger DP, Dengler WA, Fiebig GH (1996) Direct cytotoxic effects of preparations used as unconventional methods in the clonogenic assay and in nude mice. Contrib Oncol 51:115–122;

    CAS  Google Scholar 

  • Drees M, Berger DP, Dengler WA, Fiebig GH (1996) Direct cytotoxic effects of preparations used as unconventional methods in the clonogenic assay and in nude mice. Chem Abstr 1996 125:75628u

    Google Scholar 

  • Encke F (1958) Dionaea L. In: Encke F (ed) Pareys Blumengärtnerei, vol 1, 2nd edn. Paul Parey, Berlin, pp 713–714

    Google Scholar 

  • Finnie JF, van Staden J (1993) Drosera spp. (Sundew): micropropagation and the in vitro production of plumbagin. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 24. Medicinal and aromatic plants V. Springer, Berlin Heidelberg New York, pp 164–177

    Chapter  Google Scholar 

  • Fujii N, Yamashita Y, Arima Y, Nagashima M, Nakano H (1992) Induction of topoisomerase IImediated DNA cleavage by the plant naphthoquinones plumbagin and shikonin. Antimicrob Agents Chemother 36:2589–2594

    Article  PubMed  CAS  Google Scholar 

  • Galek H, Osswald WF, Elstner EF (1990) Oxidative protein modification as predigestive mechanism of the carnivorous plant Dionaea muscipula: an hypothesis based on in vitro experiments. Free Radical Biol Med 9:427–434

    Article  CAS  Google Scholar 

  • Gershbein LL, Brown MF, Hoepsch JP, Young DC (1995) Sterol composition and distribution in carnivorous plants, Sarracenia flava, Sarracenia purpurea and Dionaea muscipula. Trans Ill Acad Sci 88:13–20;

    CAS  Google Scholar 

  • Gershbein LL, Brown MF, Hoepsch JP, Young DC (1995) Sterol composition and distribution in carnivorous plants, Sarracenia flava, Sarracenia purpurea and Dionaea muscipula. Chem Abstr 1995 123:52269x

    Google Scholar 

  • Gujar GT (1990) Plumbagin, a naturally occurring naphtoquinone. Its pharmacological and pesticidal activity. Fitoterapia 61:387–394

    Google Scholar 

  • Hamlet EH (1995) Dionaea. In: Cullen J, Alexander JCM, Brady A (eds) The European garden flora, vol 4, part 2. Dicotyledons. Cambridge Univ Press, Cambridge, p 88

    Google Scholar 

  • Hauser SP (1988) Carnivora — Phytotherapeuticum zur Behandlung maligner Erkrankungen. Schweiz Rundsch Med (PRAXIS) 77:283–287

    Google Scholar 

  • Hegnauer R (1989) Chemotaxonomie der Pflanzen, vol 8. Birkhäuser, Basel

    Google Scholar 

  • Hegnauer R (1990) Chemotaxonomie der Pflanzen, vol 9. Birkhäuser, Basel

    Google Scholar 

  • Hodick D, Sievers A (1986) The influence of Ca2+ on the action potentials in mesophyll cells of Dionaea muscipula Ellis. Protoplasma 133:83–84

    Article  Google Scholar 

  • Hodick D, Sievers A (1989) Action potentials of Dionaea muscipula Ellis. Planta 179:32–34

    Article  Google Scholar 

  • Hutchinson JF (1984) In vitro propagation of Dionaea muscipula Ellis (Venus fly trap). Sci Hortic 22:189–194

    Article  Google Scholar 

  • Ichiishi S, Nagamitsu T, Kondo Y, Iwashina T, Kondo K, Tagashira N (1999) Effects of macrocomponents and sucrose in the medium on in vitro red-color pigmentation in Dionaea muscipula Ellis and Drosera spathulata Labill. Plant Biotechnol (Tokyo) 16 (3):235–238

    Article  CAS  Google Scholar 

  • Jay M, Lebreton P (1972) Chemotaxonomic research on vascular plants 26. The flavonoids of Sarraceniaceae, Nepenthaceae, Droseraceae and Cephalotaceae, a critical study of the order Sarraceniales. Nat Canadienne 99:607–613

    Google Scholar 

  • Josephy PD, Snieckus VA (1992) Ellagic acid and synthetic analogs as inhibitors of mutagenesis and carcinogenesis. Cell Mol Targets Chemoprev 1992:147–160

    Google Scholar 

  • Juniper BE, Robins RJ, Joel DM (1989) The carnivorous plants. Academic Press, London

    Google Scholar 

  • Keller H (1987) Pharmaceutical containing the digestive juices of carnivorous plants for treatment of malignant and chronic diseases. Ger Offen DE 1987 3:619;

    Google Scholar 

  • Keller H (1987) Pharmaceutical containing the digestive juices of carnivorous plants for treatment of malignant and chronic diseases. Chem Abstr 1988 109:79697h

    Google Scholar 

  • Kreher B, Neszmelyi A, Polos K, Wagner H (1988a) Structure elucidation of plumbagin-analogues from Dionaea muscipula and their in vitro immuno-modulating activities in vitro and in vivo. Proc Int Symp Hungarian Chem Soc, Sopron (Hungary) 24–27.06.88, pp 1–16

    Google Scholar 

  • Kreher B, Neszmelyi A, Polos K, Wagner H (1988b) Structure elucidation of plumbagin analogues from Dionaea muscipula and their in vitro immunological activity on human granulocytes and lymphocytes. 36th Annu Congr Med Plant Res, Freiburg, Abstr, p 45

    Google Scholar 

  • Kreher B, Neszmelyi A, Wagner H (1990) Naphthoquinones from Dionaea muscipula. Phytochemistry 29:605–606

    Article  CAS  Google Scholar 

  • Kukułczanka K (1991) Micropropagation and in vitro germplasm storage of Droseraceae. Bot Gard Micropropagation News 1:38–42

    Google Scholar 

  • Kukulczanka K, Czḁstka B (1987) Propagation of Drosera L. in vitro conditions, Wiad Bot Biul Ogrodów Bot 31:61–64

    Google Scholar 

  • Kukułczanka K, Czastka B (1991) Vegetative propagation of selected species of Droseraceae taxa and in vitro gene bank establishment. Prace Ogr Bot PAN 1:55–61

    Google Scholar 

  • Kukułczanka K, Czḁstka B, Arczewska A (1989) Regeneration from leaves of Dionaea muscipula Ellis cultured in vitro. Acta Hortic 251:155–160

    Google Scholar 

  • Kukułczanka K, Kromer K, Klonowska B, Czastka B (1991) The temperature effect on the preservation of the plant cultures of Droseraceae in vitro. Prace Ogr Bot PAN 1:63–68

    Google Scholar 

  • Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100–127

    Article  CAS  Google Scholar 

  • Lloyd FE (1942) The carnivorous plants. Chronica Botanica. Waltham, MA, USA, pp 177–194

    Google Scholar 

  • Miles DH, Kokpol U, Bhattacharayya J, Attwood JL, Stone KE, Bryson TA, Wilson C (1976) Structure of sarracenin. An unusual enol diacetal monoterpene from the insectivorous plant Sarracenia flava. J Am Chem Soc 98:1569–1573

    Article  CAS  Google Scholar 

  • Miyoshi E, Shizuri Y, Yamamura S (1984) Isolation and structures of diomuscinone and diomuscipulone from Dionaea muscipula. Phytochemistry 23:2385–2387

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Pakulski G (1994) Zwiazki fenolowe z gatunku Dionaea muscipula Ellis pochodzḁcego z kultur in vitro. (Phenolic compounds from the species Dionaea muscipula Ellis of in vitro culture origin) Dissertation, Faculty of Pharmacy, K.Marcinkowski University of Medical Sciences, Poznań

    Google Scholar 

  • Pakulski G, Budzianowski J (1996a) Ellagic acid derivatives and naphthoquinones of Dionaea muscipula from in vitro cultures. Phytochemistry 41:775–778

    Article  CAS  Google Scholar 

  • Pakulski G, Budzianowski J (1996b) Flavonoids of Dionaea muscipula from in vitro cultures. Planta Med 62:95–96

    Article  PubMed  CAS  Google Scholar 

  • Parliman BJ, Evans PT, Rupert EA (1982a) Tissue culture of single rhizome explants of Dionaea muscipula Ellis ex L., the Venus fly-trap, for rapid asexual propagation. J Am Soc Hortic Sci 107 (2):305–310

    CAS  Google Scholar 

  • Parliman RJ, Evans PT, Mazur AR (1982b) Adventitious bud differentiation and development in leaf cuttings of Dionaea muscipula Ellis ex L. (Venus fly-trap) cultured in vitro. J Am Soc Hortic Sci 107 (2):310–316

    CAS  Google Scholar 

  • Ragazzi E, de Biasi M, Pandolfo L, Chinellato A, Caparrotta L (1993) In vitro effects of naphthoquinones isolated from Drosera species. Pharmacol Res 27(Suppl 1):87–88

    Article  CAS  Google Scholar 

  • Reinert RA, Mohr HC (1967) Propagation of Cattleya by tissue culture of lateral bud meristems. Proc Am Soc Hortic 91:664–671

    Google Scholar 

  • Rote Liste (2000) Verzeichnis von Fertigarzneimitteln der Mitglieder des Bundesverbandes der Pharmazeutischen Industrie. Cantor, Aulendorf

    Google Scholar 

  • Samaj J, Blehová A, Repčák M, Ovečka M, Bobák M (1999) Drosera species (Sundew): in vitro culture and the production of plumbagin and other secondary metabolites. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 43. Medicinal and aromatic plants XI. Springer, Berlin Heidelberg New York, pp 105–135

    Google Scholar 

  • Sampara-Rumantir N (1971) Rossoliside. Pharm Weekbl 106:653–664

    CAS  Google Scholar 

  • Shanhinpoor M, Thompson MS (1995) The Venus flytrap as a model for a biomimetic material with built-in sensor and actuations. Mater Sci Eng C C2:229–233;

    Article  Google Scholar 

  • Shanhinpoor M, Thompson MS (1995) The Venus flytrap as a model for a biomimetic material with built-in sensor and actuations. Chem Abstr 1996 124:6160r

    Google Scholar 

  • Slack A (1985) Karnivoren: Biologie und Kultur der Insektenfangenden Pflanzen. Ulmer, Stuttgart, pp 243–245 (15–19), 167–173

    Google Scholar 

  • Teng WL (1999) Source, etiolation and orientation of explants affect in vitro regeneration of venus fly-trap (Dionaea muscipula). Plant Cell Rep 18:363–368

    Article  CAS  Google Scholar 

  • Todorov DK, Ilarianova MV (1996) Antitumor activity of the Dionaea muscipula Ellis. Preparation Carnivora New in vitro and in vivo on several animal and human tumors, sensitive and resistant to antitumor drugs. Carnivora-Forschungs GmbH Nordhalben Firm’s Information

    Google Scholar 

  • Todorov DK, Ilarianova MV, Maneva K (1995a) Antiproliferative activity of Carnivora on two human glioma cell lines. Carnivora-Forschungs GmbH Nordhalben Firm’s Information

    Google Scholar 

  • Todorov DK, Ilarianova MV, Maneva K (1995b) Antiproliferative activity of Carnivora on multidrug-resistant sarcoma 180/dox constant tumor cell line. Carnivora-Forschungs GmbH Nordhalben Firm’s Information

    Google Scholar 

  • Todorov DK, Ilarianova MV, Maneva K (1996a) Experimental studies on the antiproliferative activity of Carnivora on human leukemic CEM cell line — multidrug-resistant (MDR). Carnivora-Forschungs GmbH Nordhalben Firm’s Information

    Google Scholar 

  • Todorov DK, Ilarianova MV, Maneva K (1996b) Experimental studies on the antiproliferative activity of Carnivora on human leukemic CEM cell line — multidrug-resistant (MDR). Carnivora-Forschungs GmbH Nordhalben Firm’s Information

    Google Scholar 

  • Todorov DK, Ilarionowa MV, Timcheva KB, Pajeva IK (1998) Antitumor activity of Dionaea muscipula E. preparation Carnivora in animal and human tumors sensitive and resistant to antitumor drugs. Biotechnol Biotechnol Equip 1998 (2):61–66

    Google Scholar 

  • Trebacz K, Sievers A (1998) Action potentials evoked by light in traps of Dionaea muscipula Ellis. Plant Cell Physiol 39:369–372

    Article  CAS  Google Scholar 

  • Trebacz K, Bush MB, Hejnowicz Z, Sievers A (1996) Cydopiazonic acid disturbs the regulation of cytosolic calcium when repetitive action potentials are evoked in Dionaea traps. Planta 198:623–626

    Article  CAS  Google Scholar 

  • Vinkeborg J, Sampara-Rumantir N, Uffielie OF (1969) Presence of hydroplumbagin glucoside in Drosera rotundifolia. Pharm Weekbl 104:45–49

    Google Scholar 

  • Vinkeborg J, Sampara-Rumantir N, Uffelie OF (1970) Rossoliside, a glucoside from Drosera rotundifolia L. Pharm Weekbl 105:414

    Google Scholar 

  • Wagner H, Kreher B, Jurcic K (1986) Immunological investigations of naphthoquinone containing plant extracts, isolated quinones and other cytostatic compounds in cellular immunosystems. 34th Annu Congr on Med Plant Res, Hamburg, Germany, Abstr, pp 69–70

    Google Scholar 

  • Wagner H, Kreher B, Jurcic K (1988) In vitro stimulation of human granulocytes and lymphocytes by pico and femtogram quantities of cytostatic agents. Arzneim-Forsch Drug Res 38:273–275

    CAS  Google Scholar 

  • Wurm G, Grimm H, Geres U, Schmidt H (1984) Plumbagin: Reaktivität, toxicität und mikrobielle aktivität des in Drosera und Plumbago arten vorkommenden. Dtsch Apoth Ztg 124:2128–2132

    CAS  Google Scholar 

  • Zenk MH, Fürbringer M, Steglich W (1969) Occurence and distribution of 7-methyljuglone and plumbagin in the Droseraceae. Phytochemistry 8:2199–2200

    Article  CAS  Google Scholar 

  • Zimmerman RH, Broome OC (1980) Blueberry micropropagation. Proc Conf Nursey Culture — Applications and Feasibility, 21–22 April 1980, Beltsville, USA, pp 44–47

    Google Scholar 

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Kukułczanka, K., Budzianowski, J. (2002). Dionaea muscipula Ellis (Venus Flytrap): In Vitro Cultures and in Vitro Production of Secondary Metabolites. In: Nagata, T., Ebizuka, Y. (eds) Medicinal and Aromatic Plants XII. Biotechnology in Agriculture and Forestry, vol 51. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-08616-2_4

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