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Polymerase Chain Reaction for the Detection of Adenoviruses

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Book cover Diagnosis of Human Viruses by Polymerase Chain Reaction Technology

Part of the book series: Frontiers of Virology ((FRVIROLOGY,volume 1))

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Summary

The use of the polymerase chain reaction (PCR) for the detection of human adenoviruses in diluted stool samples was investigated. Several primers, including ones specific for the hexon-coding region and for enteric adenovirus (EAd) types 40 and 41, were evaluated. The primers constitute three different PCR systems designed for the detection of all six adenovirus subgenera (A-F), the two EAds Ad40 and Ad41, and Ad40, respectively. The general system and the EAd-specific system also include nested primers for a two-step amplification. The limit of detection of the PCR assay in a two-step amplification was 1 single virus particle when the two sets of general hexon primers or EAd-specific primers were used. The sensitivity of the PCR, together with its simplicity and reduced time-scale compared with other detection methods, emphasize the potential of this technique as an additional method for routine diagnosis of human adenovirus infections.

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Authors and Affiliations

  1. Department of Virology, University of Umeå, 901 85, Umeå, Sweden

    Annika Allard & Göran Wadell

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  1. Annika Allard
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  2. Göran Wadell
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Editors and Affiliations

  1. Department of Molecular Virology Faculty of Medicine, Hebrew University of Jerusalem, POB 1172, Jerusalem, Israel

    Yechiel Becker

  2. Institut für Medizinische Virologie, der Universität Heidelberg, Im Neuenheimer Feld 324, W-6900, Heidelberg, Germany

    Gholamreza Darai

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© 1992 Springer-Verlag Berlin Heidelberg

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Allard, A., Wadell, G. (1992). Polymerase Chain Reaction for the Detection of Adenoviruses. In: Becker, Y., Darai, G. (eds) Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Frontiers of Virology, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84766-0_23

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  • DOI: https://doi.org/10.1007/978-3-642-84766-0_23

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84768-4

  • Online ISBN: 978-3-642-84766-0

  • eBook Packages: Springer Book Archive

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