Abstract
Knowledge of genome structure is a prerequisite to the genetic engineering of trees and their subsequent clonal propagation, because some characterization of nuclear and organellar DNA must precede its manipulation. We need information on nuclear genome size and how much of the DNA is composed of highly repeated, moderately repeated, and near-unique or single-copy genes. We also need to locate and characterize genes of interest with regard to their molecular structure, repetition frequency, and base sequence. It is useful to know what genes have a highly conserved structure, making them similar in DNA sequence to genes in other organisms. Some genes are steady-state, i.e., expressed in all tissues and at all stages of development, and some are stage- or tissue-specific. Information is also required on the mechanisms regulating expression of genes in forest trees if we are to be successful in eliciting their expression in recombinant cells.
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Kriebel, H.B. (1993). Molecular Structure of Forest Trees. In: Ahuja, MR., Libby, W.J. (eds) Clonal Forestry I. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84175-0_14
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DOI: https://doi.org/10.1007/978-3-642-84175-0_14
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