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An Endonuclease Responsible for Apoptosis

  • Chapter
Apoptosis

Part of the book series: Progress in Molecular and Subcellular Biology ((PMSB,volume 16))

Abstract

Apoptosis is a form of cell death that plays important roles in physiological and pathological phenomena as diverse as embryogenesis, metamorphosis, hemopoiesis, and carcinogenesis (Kerr et al. 1972; Wyllie 1980; Berger 1985; Carson et al. 1986; Ucker 1987; Smith et al. 1989; Dive and Hickman 1991; Groux et al. 1992; Cohen et al. 1992; Tanuma et al. 1993). Although apoptosis is thought to be a gene-directed cell death, how this suicide is regulated is still unknown. Apoptosis is characterized morphologically by cell shrinkage, nuclear collapse, and cell fragmentation, known as apoptotic bodies, which is accompanied by internucleosomal DNA fragmentation (Kerr et al. 1972; Wyllie 1980). The signal transduction and determination processes in apoptosis initiated by apoptotic signals are complex and dependent on cell types and states. However, the DNA fragmentation, which is suggested to be catalyzed by a constitutive endonuclease, is a crucial process common in apoptosis, irrespective of the initial stimulus.

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References

  • Anai M, Sasaki M, Muta A, Miyagawa T (1981) Purification and properties of a neutral endo-deoxiribonuclease from guinea pig epidermis. Biochem Biophys Acta 656: 183–188

    PubMed  CAS  Google Scholar 

  • Arends MJ, Morris RG, Wyllie AH (1990) Apoptosis. The role of the endonuclease. Am J Pathol 136: 593–608

    PubMed  CAS  Google Scholar 

  • Barry MA, Eastman A (1993) Identification of deoxyribonuclease II as an endonuclease involved in apoptosis. Arch Biochem Biophys 300: 440–450

    Article  PubMed  CAS  Google Scholar 

  • Carson DA, Seto S, Wasson DB, Carrera CJ (1986) Lymphocyte disfunction after DNA damage by toxic oxygen. J Exp Med 163: 746–751

    Article  PubMed  CAS  Google Scholar 

  • Cohen JJ, Duke RC (1984) Glucocorticoid activation of a calcium-dependent endonuclease in thymocyte nuclei leads to cell death. J Immunol 132: 38–42

    PubMed  CAS  Google Scholar 

  • Cohen JJ, Duke RC, Fadok UA, Sellins KS (1992) Apoptosis and programmed cell death in immunity. Annu Res Immunol 10: 267–293

    Article  CAS  Google Scholar 

  • Dive C, Hickman (1991) Drug-target interactions: only the first step in the commitment to a programmed cell death? Br J Cancer 64: 192–196

    Article  PubMed  CAS  Google Scholar 

  • Dulaney JT, Touster O (1972) Isolation of deoxyribonuclease II from rat liver lysosomes. J Biol Chem 247: 1424–1432

    PubMed  CAS  Google Scholar 

  • Gaido ML, Cidlowski JA (1991) Identification, purification, and characterization of a calcium-dependent endonuclease (NUC18) from apoptotic rat thymocytes. J Biol Chem 266: 18580–18585

    PubMed  CAS  Google Scholar 

  • Groux H, Torpier G, Monte D, Monton Y, Capron A, Ameisen JC (1992) Activation-induced death by apoptosis in CD4+ T cells from human immunodeficiency virus-infected asymptomatic Individuals. J Exp Med 175: 331–340

    Article  PubMed  CAS  Google Scholar 

  • Hashida T, Tanaka Y, Matsunami N, Yoshihara K, Kamiya T, Tanigawa Y, Koide SS (1982) Purification and properties of bull seminal plasma Ca2+, Mg2+-dependent endonuclease. J Biol Chem 257: 13114–13119

    PubMed  CAS  Google Scholar 

  • Hibino Y, Yoneda T, Sugano N (1988) Purification and properties of a magnesium-dependent endodeoxyribonuclease endogenous to rat-liver nuclei. Biochem Biophys Acta 950: 313–320

    PubMed  CAS  Google Scholar 

  • Kerr JFR, Wyllie AH, Currie AR (1972) Apoptosis: a basic biological phenomenon with wide- ranging implications in tissue kinetics. Br J Cancer 26: 239–257

    Article  PubMed  CAS  Google Scholar 

  • Kreuder V, Dieckhoff J, Sitting M, Mannherz HG (1984) Isolation, characterization and crystallization of deoxiribonuclease I from bovine and rat parotid gland and its interaction with rabbit skeletal muscle actin. Eur J Biochem 139: 389–400

    Article  PubMed  CAS  Google Scholar 

  • Lacks SA (1981) Deoxiribonuclease I in mammalian tissues. Specificity of source. J Biol Chem 256: 2644–2648

    PubMed  CAS  Google Scholar 

  • Liao TH (1985) The subunit structure and active site sequence of porcine spleen deoxyribonuclease. J Biol Chem 260: 10708–10713

    PubMed  CAS  Google Scholar 

  • McConkey DJ, Hartzell P, Orreniuss (1990) Rapid turnover of endogenous endonuclease activity in thymocytes: effects of inhibitors of macromolecular synthesis. Arch Biochem Biophys 278: 284–287

    Article  PubMed  CAS  Google Scholar 

  • Nagae S, Nakayama J, Nakano I, Anai M (1982) Purification and properties of a neutral endodeoxyribonuclease from rat small intestinal mucosa. Biochemistry 21: 1339–1344

    Article  PubMed  CAS  Google Scholar 

  • Nakayama J, Fujiyoshi T, Nakamura M, Anai M (1981) Purification and properties of an endodeoxyribonuclease from nuclei of bovine small intestinal mucosa. J Biol Chem 256: 1636–1642

    PubMed  CAS  Google Scholar 

  • Nikonova LV, Beletsky IP, Umansky SR (1993) Properties of some nuclear nucleases of rat thymocytes and their changes in radiation-induced apoptosis. Eur J Biochem 215: 893–901

    Article  PubMed  CAS  Google Scholar 

  • Pedrini AM, Ranzani G, Pedrali Noy GCF, Spadari S, Falaschi A (1976) A novel endonuclease of human cells specific for single-stranded DNA. Eur J Biochem 70: 275–283

    Article  PubMed  CAS  Google Scholar 

  • Peitsch MC, Polzar B, Stepham H, Crompton T, MacDonald HR, Mannherz HG, Tshopp J (1993) Characterization of the endogenous deoxyribonuclease involved in nuclear DNA degradation during apoptosis (programmed cell death). EMBO J 12: 371–377

    PubMed  CAS  Google Scholar 

  • Ribeiro JM, Carson DA (1993) Ca2+/Mg2+-dependent endonuclease from human spleen: purification, properties, and role in apoptosis. Biochemistry 32: 9129–9136

    Article  PubMed  CAS  Google Scholar 

  • Shiokawa D, Ohyama H, Yamada T, Takahashi K, Tanuma S (1994) Identification of an endonuclease responsible for apoptosis in rat thymocytes. Eur J Biochem 226: 23–30

    Article  PubMed  CAS  Google Scholar 

  • Smith CA, Williams GT, Kingston R, Jenkinson EJ, Owen JJ (1989) Antibodies to CD3/T-cell receptor complex induce death by apoptosis in immature T cells in thymic cultures. Nature 337: 181–184

    Article  PubMed  CAS  Google Scholar 

  • Tanuma S, Shiokawa D (1994) Multiple forms of nuclear deoxyribonuclease in rat thymocytes. Biochem Biophys Res Commun 203: 789–797

    Article  PubMed  CAS  Google Scholar 

  • Tanuma S, Shiokawa D, Tanimoto Y, Ikekita M, Sakagami H, Takeda M, Fukuda S, Kochi M (1993) Benzylideneascorbate induces apoptosis in L929 tumor cells. Biochem Biophys Res Commun 194: 29–35

    Article  PubMed  CAS  Google Scholar 

  • Ucker DS (1987) Cytotoxic T lymphocytes and glucocorticoid activate an endogenous suicide process in target cells. Nature 327: 62–64

    Article  PubMed  CAS  Google Scholar 

  • Wang E, Furth JJ (1977) Mammalian endonuclease, DNase V. Purification and properties of enzyme of calf thymus. J Biol chem 252: 116–124

    PubMed  CAS  Google Scholar 

  • Wyllie AH (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284: 555–556

    Article  PubMed  CAS  Google Scholar 

  • Wyllie AH, Morris RG, Smith AL, Dunlop D (1984) Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis. J Pathol 142: 67–77

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Biochemistry, Faculty of Pharmaceutical Science, Science University of Tokyo, Shinjuku-ku, Tokyo, Japan

    S. Tanuma & D. Shiokawa

  2. Research Institute for Bioscience, Science University of Tokyo, Noda, Chiba, Japan

    S. Tanuma

Authors
  1. S. Tanuma
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  2. D. Shiokawa
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Editor information

Editors and Affiliations

  1. Dept. of Biophysics, National Cancer Center Research Institute, Tsukiji 5-Chome, Chuoko, Tokyo, Japan

    Y. Kuchino

  2. Abteilung für Angewandte Molekularbiologie, Universität Mainz, Institut für Physiologische Chemie, Duesbergweg 6, 55099, Mainz, Germany

    W. E. G. Müller

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© 1996 Springer-Verlag Berlin Heidelberg

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Tanuma, S., Shiokawa, D. (1996). An Endonuclease Responsible for Apoptosis. In: Kuchino, Y., Müller, W.E.G. (eds) Apoptosis. Progress in Molecular and Subcellular Biology, vol 16. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79850-4_1

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  • DOI: https://doi.org/10.1007/978-3-642-79850-4_1

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-79852-8

  • Online ISBN: 978-3-642-79850-4

  • eBook Packages: Springer Book Archive

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