Abstract
The purpose of this presentation is to describe procedures of analysis of final extents and kinetics of virus fusion with target membranes. The presentation of results will focus on deductions from studies of final extents of fusion.
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References
Amselem S, Barenholz Y, Loyter A, Nir S, Lichtenberg D (1986) Fusion of Sendai virus with negatively charged liposomes as studied by pyrene-labelled phospholipid liposomes. Biochim Biophys Acta 860: 301–313
Bentz J, Nir S, Covell D (1988) Mass action kinetics of virus-cell aggregation and fusion. Biophys J 54: 449–462
Bentz J (1992) Intermediates and kinetics of membrane fusion. Biophys J 63:448–459
Cheetham JJ, Nir S, Johnson E, Flanagan TD, Epand RM (1994) The effects of membrane physical properties on the fusion of Sendai virus with human erythrocyte ghosts and liposomes: Analysis of kinetics and extent of fusion. J Biol Chem 269: 5467–5472
Düzgüne§ N, Lima MCP, Stamatatos L, Flasher D, Alford D, Friend DS, Nir S (1992) Fusion of influenza virus with human promyelocytic leukemia and lymphoblastic leukemia cell, and murine lymphoma cells: Kinetics of low pH-induced fusion monitored by fluorescence dequenching. J Gen Virol 73: 27–37
Fonteijn TAA, Engberts JBFN, Nir S, Hoekstra D (1992) Asymmetric fusion betwen synthetic Dl-N-Dodecyl phosphate vesicles and virus membranes. Biochim Biophys Acta 1110: 185–192
Hoekstra D, DeBoer T, Klappe K, Wilschut J (1984) Fluorescence method for measuring the kinetics of fusion between biological membranes. Biochemistry 23: 5675–5681
Hoekstra D, Klappe K (1986) Sendai virus-erythrocyte membrane interaction: quantitative and kinetic analysis of viral binding, dissociation, and fusion. J Virol 58: 87–95
Hoekstra D, Klappe K, Hoff H, Nir S (1989) Mechanism of fusion of Sendai virus: Role of hydrophobic interactions and mobility constraints of viral membrane proteins. Effects of polyethylene glycol. J Biol Chem 264: 6786–6792
Klappe K, Wilschut J, Nir S, Hoekstra D (1986) Parameters affecting fusion between Sendai virus and liposomes. Role of viral proteins, liposome composition and pH. Biochemistry 25: 8252–8260
Larsen CE, Alford DR, Young LJT, McGraw TP, Düzgüneş, N (1990) Fusion of simian immunodeficiency virus with liposomes and erythrocyte ghost membranes: Effects of lipid composition, pH and calcium. J Gen Virol 71: 1947–1955
Larsen CE, Nir S, Alford DR, Jennings M, Lee K, Düzgünes, N (1993) Human immunodeficiency virus type 1 (HIV-1) fusion with model membranes: Kinetic analysis and the role of lipid composition, pH and divalent cations. Biochim Biophys Acta 1147: 223–236
Lima MCP, Ramalho-Santos J, Martins MF, Carvalho AP, Bairos VA, Nir S (1992) Kinetic modeling of Sendai virus fusion with PC-12 cells: Effect of pH and temperatue on fusion and viral inactivation. Eur J Biochem 205: 181–186
Nir S, Stegmann T, Wilschut J (1986a) Fusion of influenza virus and cardiolipin liposomes at low pH: Mass action analysis of membrane lipid mixing and aqueous contents mixing. Biochemistry 25: 257–266
Nir S, Klappe K, Hoekstra D (1986b) Kinetics of fusion between Sendai virus and erythrocyte ghosts: Application of mass action kinetic model. Biochemistry 25: 2155–2161
Nir S, Klappe K, Hoekstra D (1986c) Mass action analysis of kinetics and extent of fusion between Sendai virus and phospholipid vesicles. Biochemistry 25: 8261–8266
Nir S, Stegmann T, Hoekstra D, Wilschut J (1988) Kinetics and extents of fusion of influenza virus and Sendai virus with liposomes, in Molecular Mechanisms of Membrane Fusion (Ohki S, Doyle D, Flanagan TD, Hui SW, & Mayhew E, eds) Plenum Press New York
Nir S, Düzgüne§ N, Lima MCP, Hoekstra D (1990) Fusion of enveloped viruses with cells and liposomes. Cell Biophys 17: 181–201
Nir S (1993) Analysis of kinetics and extent of fusion of viruses with target membranes. in Methods in Enzymology 220, (Düzgüneş N, ed) Academic Press New York
Ramalho-Santos J, Nir S, Düzgüne§ N, Carvalho AP, Lima MCP (1993) A common mechanism for virus fusion activity and inactivation. Biochemistry 32: 2771–2779
Stegmann T, Nir S, Wilschut J (1989) Membrane fusion activity of influenza virus. Effects of gangliosides and negatively charged phospholipids in target liposomes. Biochemistry 28: 1698–1704
Stegmann T, White J, Helenius A (1990) Intermediates in influenza induced membrane fusion. EMBO J 9: 4231–4241
Struck DK, Hoekstra D, Pagano RE (1981) Use of resonance energy transfer to monitor memebrane fusion. Biochemistry 20: 4093–4099
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© 1995 Springer-Verlag Berlin Heidelberg
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Nir, S., Düzgüneş, N., Hoekstra, D., Ramalho-Santos, J., Pedroso de Lima, M.C. (1995). Mass action model of virus fusion. In: De Lima, M.C.P., Düzgüneş, N., Hoekstra, D. (eds) Trafficking of Intracellular Membranes:. NATO ASI Series, vol 91. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-79547-3_9
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DOI: https://doi.org/10.1007/978-3-642-79547-3_9
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