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Transformation of Tobacco (Nicotiana clevelandii and N. Benthamiana)

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Plant Protoplasts and Genetic Engineering IV

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 23))

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Abstract

A range of procedures has been developed for transferring specific DNA into plant cells. These include the uptake of DNA by protoplasts (Krens et al. 1982; Potrykus et al. 1985), DNA transfer mediated by Agrobacterium, and more recently the microprojectile (Klein et al. 1987) or shoot gun method, which is of great interest for the transformation of plants whose tissues are recalcitrant to regeneration of whole plants from cells.

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Authors and Affiliations

  1. ENEA Dip. Ricerca e Sviluppo Agroindustriali, Div. Ingegneria Genetica, C.P. 2400, 00100, Roma A.D, Italy

    G. Ancora

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  1. R. Tavazza
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  2. G. Ancora
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Editors and Affiliations

  1. A-137, New Friends Colony, 110065, New Delhi, India

    Y. P. S. Bajaj

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© 1993 Springer-Verlag Berlin Heidelberg

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Tavazza, R., Ancora, G. (1993). Transformation of Tobacco (Nicotiana clevelandii and N. Benthamiana). In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering IV. Biotechnology in Agriculture and Forestry, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-78037-0_23

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  • DOI: https://doi.org/10.1007/978-3-642-78037-0_23

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-78039-4

  • Online ISBN: 978-3-642-78037-0

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