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Analytical Applications of UV-VIS Spectroscopy

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Book cover UV-VIS Spectroscopy and Its Applications

Part of the book series: Springer Lab Manuals ((SLM))

Abstract

For about 130 years, the Bouguer-Lambert-Beer law has been used as the quantitative basis of absorption spectroscopy. Bouguer established empirically a correlation between pathlength and light absorption in 1729. This correlation was then formulated mathematically by Lambert in 1760 [1] and Beer discovered the dependence upon the concentration in 1852 [2]. Initially, the human eye was the detector for comparing different light intensities. In 1925, Pulfrich [3] introduced his photometer as “a photometer appropriately adapted to exceed the levels of sensitivity of the human eye, called a step photometer…”. Our eyes are capable of assessing the uniformity of two light densities with an accuracy of approximately 1%. The principle of colorimetry or visual photometry is based on this fact (see [4–6] etc.).

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  1. Heinrich-Heine-Universität Physikalische Chemie und Elektrochemie I, Universitätsstraβe 26.43.02, W-4000, Düsseldorf 1, Germany

    Profosser em. Dr. Heinz-Helmut Perkampus

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Perkampus, HH. (1992). Analytical Applications of UV-VIS Spectroscopy. In: UV-VIS Spectroscopy and Its Applications. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77477-5_4

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